C57BL/6JCya-Rictorem1flox/Cya
Common Name:
Rictor-flox
Product ID:
S-CKO-16854
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rictor-flox
Strain ID
CKOCMP-78757-Rictor-B6J-VA
Gene Name
Product ID
S-CKO-16854
Gene Alias
4921505C17Rik; 6030405M08Rik; AVO3; D530039E11Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rictorem1flox/Cya mice (Catalog S-CKO-16854) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061656
NCBI RefSeq
NM_030168
Target Region
Exon 4~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Rictor, short for Rapamycin-insensitive companion of mammalian target of rapamycin, is a key component of the mTORC2 complex. mTORC2 is involved in multiple cellular processes, such as cell survival, migration, and metabolism. It is part of the phosphatidylinositol 3-kinases (PI3K) pathway, and its activation is crucial for the phosphorylation of Akt/PKB at Ser473, which is important for the regulation of various downstream signaling cascades related to cancer and diabetes [2].
In melanoma, low RICTOR levels in BRAF-mutated tumors correlate with a worse clinical outcome. RICTOR-deficient BRAFV600E cells are tolerant to BRAF/MEK inhibitors and show enhanced mitochondrial respiration and NAMPT expression [1].
In renal allograft, Rictor/mTORC2 signaling contributes to interstitial fibrosis by regulating BNIP3-mediated mitophagy. Rictor knockout in endothelial cells alleviates renal vascular endothelial-to-mesenchymal transition and allograft interstitial fibrosis [3].
In lung cancer, amplification of the RICTOR gene and overexpression of Rictor protein can promote cell survival and migration, serving as a potential druggable target [4].
In hepatocellular carcinoma, Rictor binds to wild-type p53 and blocks its activity, playing an oncogenic role. The dynamic nucleocytoplasmic distribution of Rictor is observed during malignant transformation [5].
In conclusion, Rictor, as an essential part of mTORC2, plays a significant role in various biological processes and disease conditions. Gene knockout models in mice have revealed its role in melanoma, renal allograft fibrosis, lung cancer, and hepatocellular carcinoma, providing potential therapeutic targets for these diseases.
References:
1. Ponzone, Luca, Audrito, Valentina, Landi, Claudia, Cavallo, Federica, Calautti, Enzo. 2024. RICTOR/mTORC2 downregulation in BRAFV600E melanoma cells promotes resistance to BRAF/MEK inhibition. In Molecular cancer, 23, 105. doi:10.1186/s12943-024-02010-1. https://pubmed.ncbi.nlm.nih.gov/38755661/
2. Sarbassov, D D, Guertin, David A, Ali, Siraj M, Sabatini, David M. . Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. In Science (New York, N.Y.), 307, 1098-101. doi:. https://pubmed.ncbi.nlm.nih.gov/15718470/
3. Feng, Dengyuan, Gui, Zeping, Xu, Zhen, Gu, Min, Tan, Ruoyun. . Rictor/mTORC2 signalling contributes to renal vascular endothelial-to-mesenchymal transition and renal allograft interstitial fibrosis by regulating BNIP3-mediated mitophagy. In Clinical and translational medicine, 14, e1686. doi:10.1002/ctm2.1686. https://pubmed.ncbi.nlm.nih.gov/38769658/
4. Szalai, Fatime, Sztankovics, Dániel, Krencz, Ildikó, Sebestyén, Anna, Khoor, Andras. 2024. Rictor-A Mediator of Progression and Metastasis in Lung Cancer. In Cancers, 16, . doi:10.3390/cancers16030543. https://pubmed.ncbi.nlm.nih.gov/38339294/
5. Wang, Chun, Kang, Hui, Yi, Yun, Zhao, Qiu, Chang, Ying. 2023. Rictor mediates p53 deactivation to facilitate the malignant transformation of hepatocytes and promote hepatocarcinogenesis. In Journal of translational medicine, 21, 919. doi:10.1186/s12967-023-04799-9. https://pubmed.ncbi.nlm.nih.gov/38110956/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen