C57BL/6JCya-Vsig1em1flox/Cya
Common Name:
Vsig1-flox
Product ID:
S-CKO-16865
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Vsig1-flox
Strain ID
CKOCMP-78789-Vsig1-B6J-VA
Gene Name
Product ID
S-CKO-16865
Gene Alias
1700062D20Rik; 4930405J24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vsig1em1flox/Cya mice (Catalog S-CKO-16865) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033806
NCBI RefSeq
NM_030181
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Vsig1, a cell-cell adhesion molecule, is a member of the immunoglobulin superfamily. It is preferentially expressed in the stomach, testis, and certain cancers such as gastric, esophageal, and ovarian cancers [4]. In the stomach, it is involved in the proper differentiation of glandular gastric epithelia, being restricted to the adherens junction of the glandular epithelium [4]. In testis, it is located on the testicular germ cell surface and its extracellular domain interacts with Sertoli cell membrane protein while its PDZ-binding motif forms a complex with ZO-1 [7].
In gastric cancer, several studies have explored its role. Loss of VSIG1 expression is significantly correlated with poor overall and disease-free survival, and negative VSIG1 expression is related to tumor size, T, and M stage, suggesting it may be a prognostic marker and potential therapeutic target [5]. Also, its membrane-cytoplasmic translocation can indicate epithelial-mesenchymal transition due to the activation of the canonical Wnt/β-catenin signaling pathway [1]. In hepatocellular carcinoma, VSIG1 may be involved in the lineage shift to gastric-type HCC, and the E-cadherin/VSIG1 complex may help suppress tumor growth [6]. In colon cancer, VSIG1 restricts the development of pro-metastatic states, in part by inhibiting YAP/TAZ-TEAD signaling [2]. A study on VSIG1 knockout (KO) mice found that VSIG1 may not play essential roles in spermatogenesis and normal testis development, function, and maintenance in mice [3].
In conclusion, Vsig1 has important functions in the proper differentiation of glandular gastric epithelia and in testis cell interactions. In cancer, its expression and translocation are associated with disease progression in gastric and hepatocellular carcinomas, and it has an anti-metastatic role in colon cancer. The Vsig1 KO mouse model has been valuable in revealing its dispensability in spermatogenesis and male fertility in mice, contributing to our understanding of its role in specific biological processes and disease conditions.
References：
1. Satala, Catalin-Bogdan, Jung, Ioan, Kovacs, Zsolt, Patrichi, Andrei-Ionut, Gurzu, Simona. 2022. V-set and immunoglobulin domain containing 1 (VSIG1) as an emerging target for epithelial-mesenchymal transition of gastric cancer. In Scientific reports, 12, 16241. doi:10.1038/s41598-022-19883-1. https://pubmed.ncbi.nlm.nih.gov/36171238/
2. Bernal, Carolina, Silvano, Marianna, Tapponnier, Yann, Angulo, Cecilia, Ruiz i Altaba, Ariel. . Functional Pro-metastatic Heterogeneity Revealed by Spiked-scRNAseq Is Shaped by Cancer Cell Interactions and Restricted by VSIG1. In Cell reports, 33, 108372. doi:10.1016/j.celrep.2020.108372. https://pubmed.ncbi.nlm.nih.gov/33176137/
3. Jung, Yena, Bang, Hyewon, Kim, Young-Hyun, Kim, Sun-Uk, Kim, Ekyune. 2021. V-Set and Immunoglobulin Domain-Containing 1 (VSIG1), Predominantly Expressed in Testicular Germ Cells, Is Dispensable for Spermatogenesis and Male Fertility in Mice. In Animals : an open access journal from MDPI, 11, . doi:10.3390/ani11041037. https://pubmed.ncbi.nlm.nih.gov/33916888/
4. Oidovsambuu, Odgerel, Nyamsuren, Gunsmaa, Liu, Shuai, Engel, Wolfgang, Adham, Ibrahim M. 2011. Adhesion protein VSIG1 is required for the proper differentiation of glandular gastric epithelia. In PloS one, 6, e25908. doi:10.1371/journal.pone.0025908. https://pubmed.ncbi.nlm.nih.gov/21991385/
5. Chen, Yibing, Pan, Ke, Li, Shengping, He, Jia, Li, Qiao. 2011. Decreased expression of V-set and immunoglobulin domain containing 1 (VSIG1) is associated with poor prognosis in primary gastric cancer. In Journal of surgical oncology, 106, 286-93. doi:10.1002/jso.22150. https://pubmed.ncbi.nlm.nih.gov/22095633/
6. Gurzu, Simona, Sugimura, Haruhiko, Szederjesi, Janos, Fodor, Decebal, Jung, Ioan. 2021. Interaction between cadherins, vimentin, and V-set and immunoglobulin domain containing 1 in gastric-type hepatocellular carcinoma. In Histochemistry and cell biology, 156, 377-390. doi:10.1007/s00418-021-02006-8. https://pubmed.ncbi.nlm.nih.gov/34170400/
7. Kim, Ekyune, Lee, Youngjeon, Kim, Ji-Su, Hong, Yonggeun, Chang, Kyu-Tae. 2010. Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1. In Molecules and cells, 30, 443-8. doi:10.1007/s10059-010-0138-4. https://pubmed.ncbi.nlm.nih.gov/20957455/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen