CRISPLD2, also known as LGL1, is a gene encoding a secreted protein. It has been implicated in multiple biological processes such as lung development, endotoxin regulation, and is associated with pathways related to inflammation and cytokine function. Genetic models like zebrafish and mouse models have been valuable in studying its functions [1,3].

In human airway smooth muscle cells, dexamethasone treatment increases CRISPLD2 expression, and its knockdown further elevates IL1β-induced IL6 and IL8 expression, suggesting it modulates the anti-inflammatory effects of glucocorticoids [1]. In zebrafish, knockdown of crispld2 leads to abnormal neural crest cell migration and increased cell death during craniofacial development [3]. In human mesenchymal stem cells, decreased CRISPLD2 expression impairs osteogenic differentiation during in vitro expansion [2].

In conclusion, CRISPLD2 plays essential roles in multiple biological processes including inflammation regulation, craniofacial development, and osteogenic differentiation. Studies using gene-knockdown models in different organisms have revealed its significance in these areas, providing insights into potential disease mechanisms related to inflammation, craniofacial anomalies, and bone-related diseases.