C57BL/6JCya-Osbpl5em1flox/Cya
Common Name:
Osbpl5-flox
Product ID:
S-CKO-16911
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Osbpl5-flox
Strain ID
CKOCMP-79196-Osbpl5-B6J-VA
Gene Name
Product ID
S-CKO-16911
Gene Alias
1110006M06Rik; ORP5; Obph1; Osbp2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Osbpl5em1flox/Cya mice (Catalog S-CKO-16911) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020411
NCBI RefSeq
NM_024289
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Osbpl5, or oxysterol binding protein like 5, is involved in lipid metabolism and phosphatidylinositol homeostasis. It may play a role in pathways related to cell survival, such as the AKT survival pathway [2]. The gene is also associated with cholesterol transport [3].
In non-small cell lung cancer (NSCLC), knockdown of Osbpl5 suppressed cell proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) process. It was identified as a downstream target of miR-526b-3p, and lncRNA LMCD1-AS1 served as a sponge for miR-526b-3p, forming an axis that promotes NSCLC cell progression [1].
In transient cerebral ischemia, conditional knockout mice showed that abolishing the Osbpl5 gene (as part of a circRNA-mediated pathway) significantly inhibited apoptosis and autophagy, suggesting its role in phosphatidylinositol homeostasis between the plasma membrane and endoplasmic reticulum [2].
In ovarian cancer, knockdown of ARL4C, which affects Osbpl5 expression via the Notch-RBP-Jκ-H3K4Me3 pathway, disrupted cholesterol transport from lysosomes to the endoplasmic reticulum and autophagy, attenuating the resistance of ovarian cancer to carboplatin [3].
In summary, Osbpl5 is crucial for lipid-related cellular functions and homeostasis. Gene-knockout and related models have revealed its significance in diseases like NSCLC, transient cerebral ischemia, and ovarian cancer drug resistance, highlighting its potential as a therapeutic target in these disease areas.
References：
1. Hu, Rui, Yu, Yankai, Wang, Haining. 2022. The LMCD1-AS1/miR-526b-3p/OSBPL5 axis promotes cell proliferation, migration and invasion in non-small cell lung cancer. In BMC pulmonary medicine, 22, 30. doi:10.1186/s12890-022-01820-7. https://pubmed.ncbi.nlm.nih.gov/35000595/
2. Shang, Fei-Fei, Luo, Li, Yan, Jianghong, He, Xiang, Liu, Wei. 2020. CircRNA_0001449 disturbs phosphatidylinositol homeostasis and AKT activity by enhancing Osbpl5 translation in transient cerebral ischemia. In Redox biology, 34, 101459. doi:10.1016/j.redox.2020.101459. https://pubmed.ncbi.nlm.nih.gov/32086008/
3. Yang, Juan, Peng, Shuping, Zhang, Keqiang. . ARL4C depletion suppresses the resistance of ovarian cancer to carboplatin by disrupting cholesterol transport and autophagy via notch-RBP-Jκ-H3K4Me3-OSBPL5. In Human & experimental toxicology, 41, 9603271221135064. doi:10.1177/09603271221135064. https://pubmed.ncbi.nlm.nih.gov/36366750/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen