C57BL/6JCya-Pum2em1flox/Cya
Common Name:
Pum2-flox
Product ID:
S-CKO-16994
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pum2-flox
Strain ID
CKOCMP-80913-Pum2-B6J-VA
Gene Name
Product ID
S-CKO-16994
Gene Alias
5730503J23Rik; Pumm2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pum2em1flox/Cya mice (Catalog S-CKO-16994) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000168361
NCBI RefSeq
NM_030723
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Pum2, short for PUMILIO2, is an RNA-binding protein that controls target mRNA turnover, often repressing translation by binding to target mRNAs in a sequence-specific manner [1,2]. It is involved in multiple biological processes and associated with various pathways, influencing gene expression at the post-transcriptional level, which is crucial for normal cellular function and development [1,2,5]. Genetic models, such as gene knockout (KO) mouse models, are valuable for studying its functions.
In a murine model of acute ischemic kidney injury, genetic deletion of Mff, whose mRNA translation is negatively regulated by Pum2, attenuated ischemic acute kidney injury-induced renal failure. Overexpression of Pum2 reduced ischemic AKI-mediated Mff upregulation and protected renal tubules [3]. In the AOM/DSS model, intestine-specific knockout of Pum2 (along with Pum1) in mice significantly inhibited the progression of colitis-associated cancer. Knockout or knockdown of Pum2 in human CRC cells decreased tumorigenicity and delayed G1/S transition [4].
In conclusion, Pum2 is a key post-transcriptional regulator involved in diverse biological processes. Model-based research, especially KO mouse models, has revealed its role in diseases like acute ischemic kidney injury and colorectal cancer. Understanding Pum2 provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Cao, Yuanping, Liu, Caiyun, Wang, Qun, Tao, Ende, Wan, Li. 2020. Pum2 mediates Sirt1 mRNA decay and exacerbates hypoxia/reoxygenation-induced cardiomyocyte apoptosis. In Experimental cell research, 393, 112058. doi:10.1016/j.yexcr.2020.112058. https://pubmed.ncbi.nlm.nih.gov/32437714/
2. Yoon, Dong Suk, Choi, Yoorim, Lee, Kyoung-Mi, Park, Kwang Hwan, Lee, Jin Woo. 2023. Downregulation of the RNA-binding protein PUM2 facilitates MSC-driven bone regeneration and prevents OVX-induced bone loss. In Journal of biomedical science, 30, 26. doi:10.1186/s12929-023-00920-8. https://pubmed.ncbi.nlm.nih.gov/37088847/
3. Wang, Jin, Zhu, Pingjun, Toan, Sam, Ren, Jun, Zhou, Hao. 2020. Pum2-Mff axis fine-tunes mitochondrial quality control in acute ischemic kidney injury. In Cell biology and toxicology, 36, 365-378. doi:10.1007/s10565-020-09513-9. https://pubmed.ncbi.nlm.nih.gov/31993882/
4. Gong, Yuanyuan, Liu, Zukai, Yuan, Yihang, Lin, Haifan, Liu, Sanhong. 2022. PUMILIO proteins promote colorectal cancer growth via suppressing p21. In Nature communications, 13, 1627. doi:10.1038/s41467-022-29309-1. https://pubmed.ncbi.nlm.nih.gov/35338151/
5. Harb, Kawssar, Richter, Melanie, Neelagandan, Nagammal, Calderon de Anda, Froylan, Duncan, Kent. 2022. Pum2 and TDP-43 refine area-specific cytoarchitecture post-mitotically and modulate translation of Sox5, Bcl11b, and Rorb mRNAs in developing mouse neocortex. In eLife, 11, . doi:10.7554/eLife.55199. https://pubmed.ncbi.nlm.nih.gov/35262486/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen