C57BL/6JCya-Kat5em1flox/Cya
Common Name
Kat5-flox
Product ID
S-CKO-17015
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-81601-Kat5-B6J-VA
When using this mouse strain in a publication, please cite “Kat5-flox Mouse (Catalog S-CKO-17015) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Kat5-flox
Strain ID
CKOCMP-81601-Kat5-B6J-VA
Gene Name
Product ID
S-CKO-17015
Gene Alias
CPLA2, Htatip, Htatip1, PLIP, Tip55, Tip60
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 19
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000113641
NCBI RefSeq
NM_178637
Target Region
Exon 1~9
Size of Effective Region
~3.2 kb
Overview of Gene Research
KAT5, also known as TIP60, is a lysine acetyltransferase that plays a crucial role in various biological processes. It is involved in pathways such as autophagy, glycolysis, innate immune response, and DNA damage repair. KAT5 mediates acetylation and lactylation of key proteins, regulating their functions and participating in important cellular programs [1,3,4].
In myocardial ischemia-reperfusion injury (MIRI) models, KAT5 was downregulated, and its overexpression inhibited NLRP3-mediated cardiomyocyte pyroptosis to relieve MIRI through modulation of the STUB1/LATS2/YAP/β-catenin axis [2]. In DNA virus infection, mice with inactivated Kat5 exhibited lower levels of serum cytokines, higher viral titers, and more susceptibility to DNA-virus-induced death, indicating that KAT5-mediated cGAS acetylation is important for the innate immune response [4]. In anaplastic thyroid cancer (ATC), KAT5 promoted invasion and metastasis through C-MYC stabilization, and its inhibitor NU9056 suppressed ATC progression through the c-Myc/miR-202 pathway [5,6]. Also, KAT5 deficiency in myeloid cells and pharmacological inhibition of KAT5 enzymatic activity reduced activation of the NLRP3 inflammasome both in vitro and in vivo [7].
In conclusion, KAT5 is essential in multiple biological processes. Its role in diseases like MIRI, response to DNA virus infection, and ATC progression has been revealed through model-based research, especially KO/CKO mouse models. These studies help understand disease mechanisms and may provide potential therapeutic targets.
References：
1. Jia, Mengshu, Yue, Xiao, Sun, Weixia, Zhao, Yuzheng, Cheng, Xiawei. 2023. ULK1-mediated metabolic reprogramming regulates Vps34 lipid kinase activity by its lactylation. In Science advances, 9, eadg4993. doi:10.1126/sciadv.adg4993. https://pubmed.ncbi.nlm.nih.gov/37267363/
2. Liu, Can, Gui, Zhongxuan, An, Cheng, Gao, Xiaotian, Ge, Shenglin. 2024. STUB1 is acetylated by KAT5 and alleviates myocardial ischemia-reperfusion injury through LATS2-YAP-β-catenin axis. In Communications biology, 7, 396. doi:10.1038/s42003-024-06086-9. https://pubmed.ncbi.nlm.nih.gov/38561411/
3. Sun, Weixia, Jia, Mengshu, Feng, Yingyan, Cheng, Xiawei. 2023. Lactate is a bridge linking glycolysis and autophagy through lactylation. In Autophagy, 19, 3240-3241. doi:10.1080/15548627.2023.2246356. https://pubmed.ncbi.nlm.nih.gov/37565742/
4. Song, Ze-Min, Lin, Heng, Yi, Xue-Mei, Hu, Ming-Ming, Shu, Hong-Bing. 2020. KAT5 acetylates cGAS to promote innate immune response to DNA virus. In Proceedings of the National Academy of Sciences of the United States of America, 117, 21568-21575. doi:10.1073/pnas.1922330117. https://pubmed.ncbi.nlm.nih.gov/32817552/
5. Wei, Xi, Cai, Shang, Boohaker, Rebecca J, Gao, Ming, Xu, Bo. . KAT5 promotes invasion and metastasis through C-MYC stabilization in ATC. In Endocrine-related cancer, 26, 141-151. doi:10.1530/ERC-18-0193. https://pubmed.ncbi.nlm.nih.gov/30400007/
6. Xu, Wenjing, Xie, Liwei, Yang, Yingying, Cai, Shang, Tian, Ye. 2022. KAT5 Inhibitor NU9056 Suppresses Anaplastic Thyroid Carcinoma Progression through c-Myc/miR-202 Pathway. In International journal of endocrinology, 2022, 2014568. doi:10.1155/2022/2014568. https://pubmed.ncbi.nlm.nih.gov/37342240/
7. Zhang, Yening, Luo, Ling, Xu, Xueming, Lu, Ben, Zhao, Kai. 2023. Acetylation is required for full activation of the NLRP3 inflammasome. In Nature communications, 14, 8396. doi:10.1038/s41467-023-44203-0. https://pubmed.ncbi.nlm.nih.gov/38110429/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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