C57BL/6JCya-Rbm5em1flox/Cya
Common Name:
Rbm5-flox
Product ID:
S-CKO-17053
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Rbm5-flox
Strain ID
CKOCMP-83486-Rbm5-B6J-VA
Gene Name
Product ID
S-CKO-17053
Gene Alias
D030069N10Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbm5em1flox/Cya mice (Catalog S-CKO-17053) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035199
NCBI RefSeq
NM_001359524
Target Region
Exon 3~4
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
Rbm5, also known as LUCA-15, is an RNA-binding protein. It is involved in the regulation of alternative splicing, a crucial process for generating diverse mRNA transcripts from a single gene, and has been implicated in apoptosis and cell proliferation regulation [3,4,6]. It may be part of the U2 snRNP complex engaged with intron branch sites in pre-mRNA splicing [1].
In ALS hSOD1G93A models, up-regulation of Rbm5 increased motor neuron apoptosis by inhibiting Rac1-mediated neuroprotection, while genetic knockdown of Rbm5 rescued motor neurons from degeneration by activating Rac1 signaling [2]. In medulloblastoma, Rbm5 acts as a tumor suppressor, and its reduced expression is associated with lower overall survival. Knockdown of Rbm5 in Daoy cells induced proliferation, while overexpression repressed cell proliferation and migration, with Rbm5 regulating Wnt/β-catenin signaling [5]. In acute myeloid leukemia, Rbm5 is highly overexpressed, and its loss significantly impairs leukemia maintenance in vitro and in vivo, as it activates the oncogenic protein HOXA9 [7]. In primary mouse neurons, Rbm5 inhibition does not modify survival in traumatic or hypoxic-ischemic injury models, but it regulates intracellular responses to injury in a sex-dependent manner [8].
In conclusion, Rbm5 is a key regulator in multiple biological processes. Its functions in apoptosis, cell proliferation, and alternative splicing have significant implications for diseases such as ALS, medulloblastoma, and acute myeloid leukemia. Studies using genetic knockdown or knockout models in various disease contexts have provided valuable insights into Rbm5's role, helping to understand the underlying molecular mechanisms and potentially guiding the development of targeted therapies.
References:
1. Damianov, Andrey, Lin, Chia-Ho, Huang, Jeffrey, Wohlschlegel, James, Black, Douglas L. 2024. The splicing regulators RBM5 and RBM10 are subunits of the U2 snRNP engaged with intron branch sites on chromatin. In Molecular cell, 84, 1496-1511.e7. doi:10.1016/j.molcel.2024.02.039. https://pubmed.ncbi.nlm.nih.gov/38537639/
2. Tan, Xingli, Su, Xiaoli, Wang, Ying, Xu, Jing, Feng, Honglin. 2024. RBM5 induces motor neuron apoptosis in hSOD1G93A-related amyotrophic lateral sclerosis by inhibiting Rac1/AKT pathways. In Brain research bulletin, 216, 111049. doi:10.1016/j.brainresbull.2024.111049. https://pubmed.ncbi.nlm.nih.gov/39142444/
3. Sutherland, Leslie C, Wang, Ke, Robinson, Andrew G. . RBM5 as a putative tumor suppressor gene for lung cancer. In Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 5, 294-8. doi:10.1097/JTO.0b013e3181c6e330. https://pubmed.ncbi.nlm.nih.gov/20186023/
4. Soni, Komal, Jagtap, Pravin Kumar Ankush, Martínez-Lumbreras, Santiago, Valcárcel, Juan, Sattler, Michael. 2023. Structural basis for specific RNA recognition by the alternative splicing factor RBM5. In Nature communications, 14, 4233. doi:10.1038/s41467-023-39961-w. https://pubmed.ncbi.nlm.nih.gov/37454201/
5. Yu, Jianzhong, Ji, Guangchun, Shi, Wei, Li, Hao, Jiang, Fei. 2020. RBM5 Acts as Tumor Suppressor in Medulloblastoma through Regulating Wnt/β-Catenin Signaling. In European neurology, 83, 242-250. doi:10.1159/000507759. https://pubmed.ncbi.nlm.nih.gov/32610314/
6. Mourtada-Maarabouni, Mirna, Williams, Gwyn T. 2002. RBM5/LUCA-15--tumour suppression by control of apoptosis and the cell cycle? In TheScientificWorldJournal, 2, 1885-90. doi:. https://pubmed.ncbi.nlm.nih.gov/12920317/
7. Zhang, Mengli, Hyle, Judith, Chen, Xiaowen, Li, Chunliang, Xu, Peng. 2024. RNA-binding protein RBM5 plays an essential role in acute myeloid leukemia by activating the oncogenic protein HOXA9. In Genome biology, 25, 16. doi:10.1186/s13059-023-03149-8. https://pubmed.ncbi.nlm.nih.gov/38216972/
8. Snyder, Kara, Gorse, Kiersten, Kochanek, Patrick M, Jackson, Travis C. 2023. Neuronal RBM5 modulates cell signaling responses to traumatic and hypoxic-ischemic injury in a sex-dependent manner. In Cell death discovery, 9, 379. doi:10.1038/s41420-023-01677-7. https://pubmed.ncbi.nlm.nih.gov/37848418/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen