C57BL/6JCya-Smarcd1em1flox/Cya
Common Name:
Smarcd1-flox
Product ID:
S-CKO-17084
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Smarcd1-flox
Strain ID
CKOCMP-83797-Smarcd1-B6J-VA
Gene Name
Product ID
S-CKO-17084
Gene Alias
Baf60a; D15Kz1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Smarcd1em1flox/Cya mice (Catalog S-CKO-17084) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023759
NCBI RefSeq
NM_031842
Target Region
Exon 7~9
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Smarcd1, also known as BAF60a, is a member of the SWI/SNF chromatin-remodeling complex family. It regulates the transcription of target genes by altering chromatin structure, and is involved in multiple biological processes such as cell differentiation, development, and senescence-associated lipid accumulation [5]. It plays a crucial role in determining cell fate, and genetic models like KO/CKO mouse models are valuable for studying its functions.
In murine adult hematopoiesis, acute deletion of Smarcd1 leads to lymphopenia, with a near-complete absence of early lymphoid progenitors and mature B and T cells, while myeloid and erythroid lineages remain unaffected. This indicates that Smarcd1 is essential for the specification of lymphoid cell fate from multipotent progenitors, achieved by interacting with the E2a transcription factor at proximal promoters and regulating distal enhancer activity [1].
In glioblastoma cell lines and high-grade glioma patients, low expression of Smarcd1 was observed. Depletion of Smarcd1 promoted cell proliferation, invasion, and chemoresistance, while enhanced expression inhibited tumor-malignant phenotypes. There is a crosstalk between Smarcd1 and Notch1 forming a feedback loop, which is crucial in regulating glioblastoma malignant phenotypes [4].
In breast cancer, both high and low expression of Smarcd1 correlate with positive clinical outcomes, while intermediate expression significantly reduces the probability of survival. Small perturbations in Smarcd1 expression can significantly reduce metastasis in mouse models and alter splicing programs relevant to the ER+/HER2-enriched breast cancer subtype [2,3].
In conclusion, Smarcd1 is an essential chromatin remodeler that governs lymphoid cell fate, and its dysregulation is associated with various diseases including glioblastoma and breast cancer. Studies using KO/CKO mouse models have provided valuable insights into its role in these disease conditions, helping to understand the underlying mechanisms and potentially guiding the development of new treatment strategies.
References:
1. Priam, Pierre, Krasteva, Veneta, Rousseau, Philippe, Kmita, Marie, Lessard, Julie A. 2024. Smarcd1 subunit of SWI/SNF chromatin-remodeling complexes collaborates with E2a to promote murine lymphoid specification. In Developmental cell, 59, 3124-3140.e8. doi:10.1016/j.devcel.2024.08.007. https://pubmed.ncbi.nlm.nih.gov/39232562/
2. Ross, Christina, Gong, Li-Yun, Jenkins, Lisa M, Majocha, Megan, Hunter, Kent W. 2024. SMARCD1 is an essential expression-restricted metastasis modifier. In Communications biology, 7, 1299. doi:10.1038/s42003-024-07018-3. https://pubmed.ncbi.nlm.nih.gov/39390150/
3. Ross, Christina, Gong, Li-Yun, Jenkins, Lisa M, Majocha, Megan, Hunter, Kent. 2024. SMARCD1 is a "Goldilocks" metastasis modifier. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.24.577061. https://pubmed.ncbi.nlm.nih.gov/38410477/
4. Zhu, Yihao, Wang, Handong, Fei, Maoxing, Niu, Wenhao, Zhang, Li. 2020. Smarcd1 Inhibits the Malignant Phenotypes of Human Glioblastoma Cells via Crosstalk with Notch1. In Molecular neurobiology, 58, 1438-1452. doi:10.1007/s12035-020-02190-z. https://pubmed.ncbi.nlm.nih.gov/33190170/
5. Inoue, Chisato, Zhao, Chong, Tsuduki, Yumi, Nomura, Masatoshi, Katakura, Yoshinori. 2017. SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes. In NPJ aging and mechanisms of disease, 3, 11. doi:10.1038/s41514-017-0011-1. https://pubmed.ncbi.nlm.nih.gov/28868154/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen