C57BL/6JCya-Gpr137bem1flox/Cya
Common Name:
Gpr137b-flox
Product ID:
S-CKO-17090
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpr137b-flox
Strain ID
CKOCMP-83924-Gpr137b-B6J-VA
Gene Name
Product ID
S-CKO-17090
Gene Alias
C730041E01; D13Abb1e; Tm7sf1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr137bem1flox/Cya mice (Catalog S-CKO-17090) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021738
NCBI RefSeq
NM_031999
Target Region
Exon 2
Size of Effective Region
~0.5 kb
Detailed Document
Overview of Gene Research
Gpr137b, also known as TM7SF1, is an orphan G-protein-coupled receptor. It localizes to the lysosome membrane and is involved in multiple biological processes. It has a role in regulating macrophage polarization, autophagy, and is associated with pathways like the mTORC1-autophagy signaling pathway, which is crucial for cell growth and metabolism [2,3,5].
In a KO mouse model of Gpr137b, deletion led to enhanced autophagy in macrophages and reduced atherosclerotic lesions, characterized by fewer necrotic cores and less lipid accumulation, indicating its role in advanced atherosclerosis progression [1]. In RAW264 mouse macrophage cell line, Gpr137b-KO clones showed reduced IL-4-induced M2 macrophage marker gene expression, suggesting its role in M2 macrophage polarization [2]. In mouse-derived ER-Hoxb8 immortalized myeloid progenitors, decreasing Gpr137b led to increased osteoclast differentiation and bone resorption activity, and gpr137ba-deficient zebrafish also showed increased bone resorption, indicating its role as a negative regulator of osteoclast activity [4].
In conclusion, Gpr137b plays essential roles in macrophage-related functions such as polarization and autophagy, as well as in bone remodeling. The gene knockout models in mice and zebrafish have significantly contributed to understanding its functions in advanced atherosclerosis and bone-related diseases, providing insights into potential therapeutic directions for these conditions.
References:
1. Qu, Wenbo, Zhou, Xin, Jiang, Xinjian, Li, Shuijie, Tian, Jinwei. 2023. Long Noncoding RNA Gpr137b-ps Promotes Advanced Atherosclerosis via the Regulation of Autophagy in Macrophages. In Arteriosclerosis, thrombosis, and vascular biology, 43, e468-e489. doi:10.1161/ATVBAHA.123.319037. https://pubmed.ncbi.nlm.nih.gov/37767704/
2. Islam, Zohirul, Inui, Takashi, Ishibashi, Osamu. 2018. Gpr137b is an orphan G-protein-coupled receptor associated with M2 macrophage polarization. In Biochemical and biophysical research communications, 509, 657-663. doi:10.1016/j.bbrc.2018.12.140. https://pubmed.ncbi.nlm.nih.gov/30595385/
3. Gan, Lin, Seki, Akiko, Shen, Kimberle, Talbot, William S, Meyer, Tobias. 2019. The lysosomal GPCR-like protein GPR137B regulates Rag and mTORC1 localization and activity. In Nature cell biology, 21, 614-626. doi:10.1038/s41556-019-0321-6. https://pubmed.ncbi.nlm.nih.gov/31036939/
4. Urso, K, Caetano-Lopes, J, Lee, P Y, Harris, M P, Charles, J F. 2019. A role for G protein-coupled receptor 137b in bone remodeling in mouse and zebrafish. In Bone, 127, 104-113. doi:10.1016/j.bone.2019.06.002. https://pubmed.ncbi.nlm.nih.gov/31173907/
5. Gao, Jialin, Xia, Libin, Lu, Meiqing, Xu, Rang, Wang, Lizhuo. 2012. TM7SF1 (GPR137B): a novel lysosome integral membrane protein. In Molecular biology reports, 39, 8883-9. doi:10.1007/s11033-012-1755-0. https://pubmed.ncbi.nlm.nih.gov/22729905/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen