C57BL/6JCya-Cd96em1flox/Cya
Common Name
Cd96-flox
Product ID
S-CKO-17112
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-84544-Cd96-B6J-VA
When using this mouse strain in a publication, please cite “Cd96-flox Mouse (Catalog S-CKO-17112) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cd96-flox
Strain ID
CKOCMP-84544-Cd96-B6J-VA
Gene Name
Product ID
S-CKO-17112
Gene Alias
1700109I12Rik, Tactile
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 16
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000023336
NCBI RefSeq
NM_032465
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Overview of Gene Research
CD96, a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, is mainly expressed by cells of hematopoietic origin, especially T and NK cells [4]. It interacts with CD155 on target cells and is involved in regulating immune responses. CD96, along with CD226 and TIGIT, forms an immune regulatory network that fine-tunes the immune response through shared ligands and differential receptor-ligand affinities [3,5,6].
In cancer, CD96 has been extensively studied. In breast cancer, tumor cell-intrinsic CD96 enhances chemoresistance and cancer stemness by promoting mitochondrial fatty acid β-oxidation via the CD155-CD96-Src-Stat3-Opa1 pathway [1]. In cervical cancer, CD96 expression is elevated on CD8+ tumor-infiltrating lymphocytes (TILs) from patients insensitive to PD-1 blockade. Blocking CD96 can enhance the effect of PD-1 blockade, improving the function of CD8+ TILs [2]. Cd96-/-mice display hypersensitive NK-cell responses to immune challenge and significant tumor resistance, indicating its inhibitory role in immune function [6]. In giant cell arteritis, a defective CD155-CD96 immune checkpoint leads to abnormal T-cell differentiation and IL-9 production [7].
In conclusion, CD96 is a crucial immune regulator. Its role in cancer immunotherapy and autoimmune diseases has been revealed through in vivo studies, especially gene knockout mouse models. These models help us understand how CD96 affects immune cell function and disease progression, providing potential targets for treating cancer and autoimmune diseases.
References：
1. Li, Jiang, Xia, Qidong, Di, Can, Liu, Xinwei, Zhao, Qiyi. 2022. Tumor Cell-Intrinsic CD96 Mediates Chemoresistance and Cancer Stemness by Regulating Mitochondrial Fatty Acid β-Oxidation. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2202956. doi:10.1002/advs.202202956. https://pubmed.ncbi.nlm.nih.gov/36581470/
2. Wang, Yumeng, Wang, Congwen, Qiu, Junjun, Li, Guiling, Hua, Keqin. . Targeting CD96 overcomes PD-1 blockade resistance by enhancing CD8+ TIL function in cervical cancer. In Journal for immunotherapy of cancer, 10, . doi:10.1136/jitc-2021-003667. https://pubmed.ncbi.nlm.nih.gov/35288463/
3. Feng, Shikai, Isayev, Orkhan, Werner, Jens, Bazhin, Alexandr V. 2023. CD96 as a Potential Immune Regulator in Cancers. In International journal of molecular sciences, 24, . doi:10.3390/ijms24021303. https://pubmed.ncbi.nlm.nih.gov/36674817/
4. Georgiev, Hristo, Ravens, Inga, Papadogianni, Georgia, Bernhardt, Günter. 2018. Coming of Age: CD96 Emerges as Modulator of Immune Responses. In Frontiers in immunology, 9, 1072. doi:10.3389/fimmu.2018.01072. https://pubmed.ncbi.nlm.nih.gov/29868026/
5. Dougall, William C, Kurtulus, Sema, Smyth, Mark J, Anderson, Ana C. . TIGIT and CD96: new checkpoint receptor targets for cancer immunotherapy. In Immunological reviews, 276, 112-120. doi:10.1111/imr.12518. https://pubmed.ncbi.nlm.nih.gov/28258695/
6. Blake, Stephen J, Dougall, William C, Miles, John J, Teng, Michele W L, Smyth, Mark J. 2016. Molecular Pathways: Targeting CD96 and TIGIT for Cancer Immunotherapy. In Clinical cancer research : an official journal of the American Association for Cancer Research, 22, 5183-5188. doi:. https://pubmed.ncbi.nlm.nih.gov/27620276/
7. Ohtsuki, Shozo, Wang, Chenyao, Watanabe, Ryu, Goronzy, Jörg J, Weyand, Cornelia M. . Deficiency of the CD155-CD96 immune checkpoint controls IL-9 production in giant cell arteritis. In Cell reports. Medicine, 4, 101012. doi:10.1016/j.xcrm.2023.101012. https://pubmed.ncbi.nlm.nih.gov/37075705/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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