C57BL/6JCya-Pla1aem1flox/Cya
Common Name:
Pla1a-flox
Product ID:
S-CKO-17118
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pla1a-flox
Strain ID
CKOCMP-85031-Pla1a-B6J-VA
Gene Name
Product ID
S-CKO-17118
Gene Alias
Ps-pla1; Pspla1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pla1aem1flox/Cya mice (Catalog S-CKO-17118) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002926
NCBI RefSeq
NM_134102
Target Region
Exon 2
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
PLA1A, also known as phosphatidylserine-specific phospholipase A1-alpha, belongs to the pancreatic lipase family. It specifically hydrolyzes sn-1 fatty acid of phosphatidylserine (PS) or 1-acyl-lysophosphatidylserine (1-acyl-lysoPS), producing lysophosphatidylserine.
PLA1A is involved in multiple biological processes. It participates in the antiviral innate immune response by facilitating the recruitment of TANK-binding kinase 1 to mitochondria [2]. It also plays a role in immune cell regulation, such as influencing the maturation and functions of T cells, dendritic cells, macrophages, and mast cells [3].
In disease-related studies, gene-knockout mouse models have provided insights. In the mannan-induced psoriatic arthritis (MIP) mouse model, Pla1a−/− mice were less susceptible to MIP with reduced bone erosions and lower levels of cytokines like IL-17, KC, IP-10, MIP-1β, LIF, and VEGF in hind paw tissues. This indicates that PLA1A may contribute to MIP through increased activation of lymphocytes, possibly those producing IL-17 [5].
In melanoma, PLA1A expression was significantly increased during melanogenesis, positively correlated with disease severity and histopathological markers of metastatic melanoma. It can be used as a diagnostic marker for advanced and BRAF-mutated melanoma [1].
In lung adenocarcinoma, low PLA1A expression was correlated with poor prognosis. Overexpressed PLA1A suppressed the proliferation, migration, and invasion of lung adenocarcinoma cells both in vitro and in vivo [4].
In conclusion, PLA1A has diverse functions in biological processes and is implicated in various diseases. The study of Pla1a knockout mouse models has been crucial in revealing its role in psoriatic arthritis, while in other diseases like melanoma and lung adenocarcinoma, its expression patterns and functional impacts have been identified, contributing to our understanding of disease mechanisms and potential diagnostic or therapeutic applications.
References:
1. Yang, Gang, Liu, Shuya, Maghsoudloo, Mazaher, Wen, QingLian, Imani, Saber. 2021. PLA1A expression as a diagnostic marker of BRAF-mutant metastasis in melanoma cancer. In Scientific reports, 11, 6056. doi:10.1038/s41598-021-85595-7. https://pubmed.ncbi.nlm.nih.gov/33723350/
2. Gao, Xiaoxiao, Chen, Dan, Hu, Xue, Pei, Rongjuan, Chen, Xinwen. 2018. PLA1A Participates in the Antiviral Innate Immune Response by Facilitating the Recruitment of TANK-Binding Kinase 1 to Mitochondria. In Journal of innate immunity, 10, 315-327. doi:10.1159/000489832. https://pubmed.ncbi.nlm.nih.gov/30016790/
3. Zhao, Yang, Hasse, Stephan, Bourgoin, Sylvain G. 2021. Phosphatidylserine-specific phospholipase A1: A friend or the devil in disguise. In Progress in lipid research, 83, 101112. doi:10.1016/j.plipres.2021.101112. https://pubmed.ncbi.nlm.nih.gov/34166709/
4. Zhou, Yue, Chang, Meijia, Wang, Ning, Li, John Zhong, Wang, Qian. 2022. Phosphatidylserine-Specific Phospholipase A1 Limits Aggressiveness of Lung Adenocarcinoma by Lysophosphatidylserine and Protein Kinase A-Dependent Pathway. In The American journal of pathology, 192, 970-983. doi:10.1016/j.ajpath.2022.03.005. https://pubmed.ncbi.nlm.nih.gov/35358472/
5. Zhao, Yang, Aoudjit, Fawzi, Bourgoin, Sylvain G. 2022. Phospholipase A1 Member A Deficiency Alleviates Mannan-Induced Psoriatic Arthritis in Mice Model. In International journal of molecular sciences, 23, . doi:10.3390/ijms23158559. https://pubmed.ncbi.nlm.nih.gov/35955693/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen