C57BL/6JCya-Lmod2em1flox/Cya
Common Name:
Lmod2-flox
Product ID:
S-CKO-17123
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Lmod2-flox
Strain ID
CKOCMP-93677-Lmod2-B6J-VA
Gene Name
Product ID
S-CKO-17123
Gene Alias
C-Lmod
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lmod2em1flox/Cya mice (Catalog S-CKO-17123) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031694
NCBI RefSeq
NM_053098
Target Region
Exon 2
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Lmod2, or Leiomodin-2, is an actin-binding protein and a key regulator of thin-filament length in muscles [2,3,4,5,7,8]. It promotes the elongation of actin filaments through polymerization at pointed ends, which is crucial for the normal assembly of striated muscle thin filaments and is essential for life [2].
In mice, knockout (KO) of Lmod2 leads to abnormally short thin filaments in the heart, resulting in dilated cardiomyopathy (DCM), ventricular arrhythmias, and postnatal or juvenile lethality [3,4,7]. Conditional knockout (CKO) in skeletal muscle shows decreased force production in both fast-and slow-twitch muscles. In slow-twitch soleus muscle, there are shorter thin filaments, increased Lmod3 levels, and a myosin fiber type switch [1]. In human patients, biallelic variants in LMOD2 are associated with DCM, often presenting in neonates or infants [2,3,6].
In conclusion, Lmod2 is essential for the integrity of sarcomeres and intercalated discs in the heart and for effective skeletal muscle contraction. Studies using Lmod2 KO and CKO mouse models have significantly advanced our understanding of its role in DCM, highlighting its importance in the pathogenesis of this cardiac disease [1,3,4,7].
References:
1. Larrinaga, Tania M, Farman, Gerrie P, Mayfield, Rachel M, Pappas, Christopher T, Gregorio, Carol C. 2024. Lmod2 is necessary for effective skeletal muscle contraction. In Science advances, 10, eadk1890. doi:10.1126/sciadv.adk1890. https://pubmed.ncbi.nlm.nih.gov/38478604/
2. Lay, Erica, Azamian, Mahshid S, Denfield, Susan W, Bi, Weimin, Lalani, Seema R. 2022. LMOD2-related dilated cardiomyopathy presenting in late infancy. In American journal of medical genetics. Part A, 188, 1858-1862. doi:10.1002/ajmg.a.62699. https://pubmed.ncbi.nlm.nih.gov/35188328/
3. Yuen, Michaela, Worgan, Lisa, Iwanski, Jessika, Gregorio, Carol C, Cooper, Sandra T. 2022. Neonatal-lethal dilated cardiomyopathy due to a homozygous LMOD2 donor splice-site variant. In European journal of human genetics : EJHG, 30, 450-457. doi:10.1038/s41431-022-01043-8. https://pubmed.ncbi.nlm.nih.gov/35082396/
4. Li, Shuang, Mo, Kaiqi, Tian, Hong, Xu, Tian, Sun, Ling V. 2016. Lmod2 piggyBac mutant mice exhibit dilated cardiomyopathy. In Cell & bioscience, 6, 38. doi:10.1186/s13578-016-0101-y. https://pubmed.ncbi.nlm.nih.gov/27274810/
5. Kiss, Balázs, Gohlke, Jochen, Tonino, Paola, Gregorio, Carol, Granzier, Henk. 2020. Nebulin and Lmod2 are critical for specifying thin-filament length in skeletal muscle. In Science advances, 6, . doi:10.1126/sciadv.abc1992. https://pubmed.ncbi.nlm.nih.gov/33177085/
6. Sono, Reiri, Larrinaga, Tania M, Huang, Alden, Nelson, Stanly, Touma, Marlin. 2023. Whole-Exome Sequencing Identifies Homozygote Nonsense Variants in LMOD2 Gene Causing Infantile Dilated Cardiomyopathy. In Cells, 12, . doi:10.3390/cells12111455. https://pubmed.ncbi.nlm.nih.gov/37296576/
7. Pappas, Christopher T, Mayfield, Rachel M, Henderson, Christine, Granzier, Henk L, Gregorio, Carol C. 2015. Knockout of Lmod2 results in shorter thin filaments followed by dilated cardiomyopathy and juvenile lethality. In Proceedings of the National Academy of Sciences of the United States of America, 112, 13573-8. doi:10.1073/pnas.1508273112. https://pubmed.ncbi.nlm.nih.gov/26487682/
8. Zhang, Yanping, Ni, Le, Lin, Bowen, Wu, Liqun, Shi, Dan. 2021. SNX17 protects the heart from doxorubicin-induced cardiotoxicity by modulating LMOD2 degradation. In Pharmacological research, 169, 105642. doi:10.1016/j.phrs.2021.105642. https://pubmed.ncbi.nlm.nih.gov/33933636/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen