C57BL/6JCya-Trim8em1flox/Cya
Common Name:
Trim8-flox
Product ID:
S-CKO-17124
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Trim8-flox
Strain ID
CKOCMP-93679-Trim8-B6J-VA
Gene Name
Product ID
S-CKO-17124
Gene Alias
GERP; Rnf27
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim8em1flox/Cya mice (Catalog S-CKO-17124) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026008
NCBI RefSeq
NM_053100
Target Region
Exon 2~6
Size of Effective Region
~5.0 kb
Detailed Document
Overview of Gene Research
Trim8, a member of the TRIM family proteins, is an E3 ubiquitin ligase. It is involved in multiple cellular processes such as cell survival, carcinogenesis, autophagy, apoptosis, differentiation, and inflammation [3,4]. Trim8 exerts its functions through regulating pivotal cellular signaling pathways including the p53 tumor suppressor, NF-κB, and JAK-STAT pathways [4,5].
In non-alcoholic fatty liver disease (NAFLD), TRIM8 forms an E3 ligase complex with TRIB3, which catalyzes K48-linked polyubiquitination of hepatic nuclear factor 4α (HNF4α) on lysine 470, leading to HNF4α degradation and NAFLD progression. Abrogating this degradation attenuated the effect of TRIB3 in a diet-induced NAFLD model [1]. In Ewing sarcoma, TRIM8 ubiquitinates and degrades EWS/FLI, a driver fusion-TF. TRIM8 knockout led to increased EWS/FLI protein levels, which was not tolerated by the cells [2]. In adipocyte inflammation and insulin resistance, inhibiting TRIM8 alleviates the conditions by regulating the DUSP14/MAPKs pathway [6].
In conclusion, Trim8 plays crucial roles in various biological processes and diseases. Through gene knockout studies in models like mice, its functions in disease-related pathways such as in NAFLD, Ewing sarcoma, and adipocyte-related metabolic disorders have been revealed. These findings contribute to understanding disease mechanisms and developing potential therapeutic strategies.
References:
1. Xiao, Meng-Chao, Jiang, Nan, Chen, Li-Lin, Qian, Hui, Xie, Wei-Fen. 2024. TRIB3-TRIM8 complex drives NAFLD progression by regulating HNF4α stability. In Journal of hepatology, 80, 778-791. doi:10.1016/j.jhep.2023.12.029. https://pubmed.ncbi.nlm.nih.gov/38237865/
2. Seong, Bo Kyung A, Dharia, Neekesh V, Lin, Shan, Fischer, Eric S, Stegmaier, Kimberly. 2021. TRIM8 modulates the EWS/FLI oncoprotein to promote survival in Ewing sarcoma. In Cancer cell, 39, 1262-1278.e7. doi:10.1016/j.ccell.2021.07.003. https://pubmed.ncbi.nlm.nih.gov/34329586/
3. Bhaduri, Utsa, Merla, Giuseppe. 2020. Rise of TRIM8: A Molecule of Duality. In Molecular therapy. Nucleic acids, 22, 434-444. doi:10.1016/j.omtn.2020.08.034. https://pubmed.ncbi.nlm.nih.gov/33230447/
4. Marzano, Flaviana, Guerrini, Luisa, Pesole, Graziano, Sbisà, Elisabetta, Tullo, Apollonia. 2021. Emerging Roles of TRIM8 in Health and Disease. In Cells, 10, . doi:10.3390/cells10030561. https://pubmed.ncbi.nlm.nih.gov/33807506/
5. Caratozzolo, Mariano Francesco, Marzano, Flaviana, Mastropasqua, Francesca, Sbisà, Elisabetta, Tullo, Apollonia. 2017. TRIM8: Making the Right Decision between the Oncogene and Tumour Suppressor Role. In Genes, 8, . doi:10.3390/genes8120354. https://pubmed.ncbi.nlm.nih.gov/29182544/
6. Zhu, Mingxue, Pu, Junliang, Zhang, Ting, Su, Rui, Tang, Chengyong. 2024. Inhibiting TRIM8 alleviates adipocyte inflammation and insulin resistance by regulating the DUSP14/MAPKs pathway. In Adipocyte, 13, 2381262. doi:10.1080/21623945.2024.2381262. https://pubmed.ncbi.nlm.nih.gov/39039652/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen