C57BL/6NCya-Sirt1em1flox/Cya
Common Name:
Sirt1-flox
Product ID:
S-CKO-17174
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Sirt1-flox
Strain ID
CKOCMP-93759-Sirt1-B6N-VA
Gene Name
Product ID
S-CKO-17174
Gene Alias
SIR2L1; Sir2; Sir2a; Sir2alpha
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Sirt1em1flox/Cya mice (Catalog S-CKO-17174) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020257
NCBI RefSeq
NM_019812
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Sirt1, also known as silent information regulator sirtuin 1 or silencing information regulator 2 related enzyme 1, is a highly conserved NAD+-dependent deacetylase belonging to the sirtuin family [1,2]. It is a post-translational regulator that modulates multiple biological processes, such as inflammation, aging, mitochondrial biogenesis, autophagy, and cell metabolism, by deacetylating a variety of histone and non-histone proteins [1-3,7-9]. Genetic models, especially gene knockout (KO) or conditional knockout (CKO) mouse models, are valuable tools for studying Sirt1's functions in vivo.
In aging-related studies, Sirt1 expression decreases with aging in mice, and increased Sirt1 expression can extend lifespan in yeast, C. elegans, and mice, suggesting its role in the aging process [2]. In the context of inflammation, alterations in Sirt1 expression and activity are linked to inflammatory diseases [1]. In myocardial ischemia-reperfusion injury, Sirt1 is involved in regulating autophagy, which is a double-edged sword in this injury. Sirt1-mediated autophagy plays different roles at different stages of injury, and targeting this mechanism can develop new drugs [3]. In endocrine disorders, dysregulation in Sirt1-mediated autophagy contributes to the onset and progression of diseases like obesity, type-2 diabetes mellitus, etc. [4].
In conclusion, Sirt1 is a key regulator in multiple biological processes. Model-based research, especially KO/CKO mouse models, has revealed its crucial roles in aging, inflammation, myocardial ischemia-reperfusion, and endocrine disorders. Understanding Sirt1's functions provides potential therapeutic targets for these related diseases.
References:
1. Yang, Yunshu, Liu, Yang, Wang, Yunwei, Tie, Jun, Hu, Dahai. 2022. Regulation of SIRT1 and Its Roles in Inflammation. In Frontiers in immunology, 13, 831168. doi:10.3389/fimmu.2022.831168. https://pubmed.ncbi.nlm.nih.gov/35359990/
2. Chen, Cui, Zhou, Min, Ge, Yuchen, Wang, Xiaobo. 2020. SIRT1 and aging related signaling pathways. In Mechanisms of ageing and development, 187, 111215. doi:10.1016/j.mad.2020.111215. https://pubmed.ncbi.nlm.nih.gov/32084459/
3. Ding, Xiaoqing, Zhu, Chenyu, Wang, Wenhong, Ma, Chunwei, Gao, Binghong. 2023. SIRT1 is a regulator of autophagy: Implications for the progression and treatment of myocardial ischemia-reperfusion. In Pharmacological research, 199, 106957. doi:10.1016/j.phrs.2023.106957. https://pubmed.ncbi.nlm.nih.gov/37820856/
4. Kim, Ji Yong, Mondaca-Ruff, David, Singh, Sandeep, Wang, Yu. 2022. SIRT1 and Autophagy: Implications in Endocrine Disorders. In Frontiers in endocrinology, 13, 930919. doi:10.3389/fendo.2022.930919. https://pubmed.ncbi.nlm.nih.gov/35909524/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen