C57BL/6JCya-Sirt1em1flox/Cya
Common Name:
Sirt1-flox
Product ID:
S-CKO-17175
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Sirt1-flox
Strain ID
CKOCMP-93759-Sirt1-B6J-VA
Gene Name
Product ID
S-CKO-17175
Gene Alias
SIR2L1; Sir2; Sir2a; Sir2alpha
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sirt1em1flox/Cya mice (Catalog S-CKO-17175) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020257
NCBI RefSeq
NM_019812
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Sirt1, short for silent information regulator sirtuin 1, is a highly conserved NAD+-dependent deacetylase belonging to the sirtuin family. It acts as a post-translational regulator, modulating multiple biological processes such as inflammation, aging, mitochondrial biogenesis, autophagy, and more. Sirt1 affects these processes by deacetylating various proteins including histones and non-histones, and is involved in signaling pathways like NF-κB, AMPK, mTOR, P53, PGC1α, and FoxOs [1,2].
Sirt1 knockout (KO) or conditional knockout (CKO) mouse models have been used to study its role in different diseases. For example, in the context of aging, decreased Sirt1 expression in mice is associated with the aging process, while increased expression can extend lifespan [2]. In myocardial ischemia-reperfusion, Sirt1 activation protects the heart, and it regulates autophagy during this process through deacetylation of FOXOs, ATGs, and LC3 [4]. In autoimmune diseases, Sirt1 may be a novel therapeutic target as its altered functions are likely involved in the disease progression [3]. In colorectal cancer, Sirt1 is a potential therapeutic target as it regulates processes like apoptosis, autophagy, and proliferation in CRC cells [5].
In conclusion, Sirt1 is a key regulator in multiple biological processes. Model-based research, especially using KO/CKO mouse models, has revealed its crucial roles in diseases related to aging, inflammation, autoimmunity, and cancer. Understanding Sirt1's functions provides potential therapeutic strategies for these disease areas.
References:
1. Yang, Yunshu, Liu, Yang, Wang, Yunwei, Tie, Jun, Hu, Dahai. 2022. Regulation of SIRT1 and Its Roles in Inflammation. In Frontiers in immunology, 13, 831168. doi:10.3389/fimmu.2022.831168. https://pubmed.ncbi.nlm.nih.gov/35359990/
2. Chen, Cui, Zhou, Min, Ge, Yuchen, Wang, Xiaobo. 2020. SIRT1 and aging related signaling pathways. In Mechanisms of ageing and development, 187, 111215. doi:10.1016/j.mad.2020.111215. https://pubmed.ncbi.nlm.nih.gov/32084459/
3. Shen, Pan, Deng, Xuan, Chen, Zhe, Yan, Jiahui, Tu, Shenghao. 2021. SIRT1: A Potential Therapeutic Target in Autoimmune Diseases. In Frontiers in immunology, 12, 779177. doi:10.3389/fimmu.2021.779177. https://pubmed.ncbi.nlm.nih.gov/34887866/
4. Ding, Xiaoqing, Zhu, Chenyu, Wang, Wenhong, Ma, Chunwei, Gao, Binghong. 2023. SIRT1 is a regulator of autophagy: Implications for the progression and treatment of myocardial ischemia-reperfusion. In Pharmacological research, 199, 106957. doi:10.1016/j.phrs.2023.106957. https://pubmed.ncbi.nlm.nih.gov/37820856/
5. Dong, Weiwei, Lu, Jinjing, Li, You, Xi, Zhuo, Cao, Shuo. 2024. SIRT1: a novel regulator in colorectal cancer. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 178, 117176. doi:10.1016/j.biopha.2024.117176. https://pubmed.ncbi.nlm.nih.gov/39059350/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen