Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Trim33em1flox/Cya
Common Name:
Trim33-flox
Product ID:
S-CKO-17229
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Trim33-flox
Strain ID
CKOCMP-94093-Trim33-B6J-VA
Gene Name
Trim33
Product ID
S-CKO-17229
Gene Alias
8030451N04Rik; Ecto; Tif1g
Background
C57BL/6JCya
NCBI ID
94093
Modification
Conditional knockout
Chromosome
3
Phenotype
MGI:2137357
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim33em1flox/Cya mice (Catalog S-CKO-17229) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029444
NCBI RefSeq
NM_053170
Target Region
Exon 2~4
Size of Effective Region
~2.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Trim33, also known as TIF-1γ, is a transcriptional repressor and E3 ubiquitin ligase. It is involved in multiple biological processes, including regulating signaling pathways like TGF-β/SMAD, and is crucial for maintaining genome stability. It plays roles in cell differentiation, development, and homeostasis, and its dysregulation is associated with various diseases [1,5,7]. Genetic mouse models, such as gene knockout (KO) and conditional knockout (CKO), have been instrumental in studying Trim33's functions.

In KO mouse models, Trim33 has been shown to have diverse roles. Hematopoietic-specific Trim33 knockout sensitized mice to bleomycin-induced pulmonary fibrosis, indicating its role as a negative regulator of lung fibrosis [1]. In dendritic cell (DC) development, conditional deletion of Trim33 in vivo caused rapid loss of DC progenitors, pDCs, and cDC1 subset, revealing its essential role in DC differentiation [2,6]. In prostate cancer, TRIM33 knockdown sensitized PCa cells to AR antagonists, suggesting its role as an oncogenic AR coactivator [3]. In esophageal squamous cell carcinoma, TRIM33 promotes tumor growth by regulating P53-related glycolysis [4]. Loss of Trim33 in multiple myeloma is associated with genomic instability and increased sensitivity to PARP inhibitors [7]. In hepatocellular carcinoma, TRIM33 promotes susceptibility to ferroptosis through ubiquitination of TFRC [8]. Epiblast-specific Trim33 mutant embryos had cardiac defects, highlighting its role in pre-cardiogenic mesoderm development [9].

In conclusion, Trim33 is a multifunctional protein involved in cell differentiation, disease development, and maintaining genome integrity. The KO/CKO mouse models have significantly contributed to understanding its roles in diseases like pulmonary fibrosis, cancer, and multiple myeloma, providing potential therapeutic targets for these conditions.

References:
1. Boutanquoi, Pierre-Marie, Burgy, Olivier, Beltramo, Guillaume, Goirand, Françoise, Bonniaud, Philippe. 2020. TRIM33 prevents pulmonary fibrosis by impairing TGF-β1 signalling. In The European respiratory journal, 55, . doi:10.1183/13993003.01346-2019. https://pubmed.ncbi.nlm.nih.gov/32184320/
2. Tiniakou, Ioanna, Hsu, Pei-Feng, Lopez-Zepeda, Lorena S, Mazzoni, Esteban O, Reizis, Boris. 2024. Genome-wide screening identifies Trim33 as an essential regulator of dendritic cell differentiation. In Science immunology, 9, eadi1023. doi:10.1126/sciimmunol.adi1023. https://pubmed.ncbi.nlm.nih.gov/38608038/
3. Chen, Mi, Lingadahalli, Shreyas, Narwade, Nitin, Poon, Terence Chuen Wai, Cheung, Edwin. 2022. TRIM33 drives prostate tumor growth by stabilizing androgen receptor from Skp2-mediated degradation. In EMBO reports, 23, e53468. doi:10.15252/embr.202153468. https://pubmed.ncbi.nlm.nih.gov/35785414/
4. Xia, Tian, Meng, Lian, Xu, Guixuan, Sun, Hao, Chen, Hao. 2024. TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth. In Cell death & disease, 15, 740. doi:10.1038/s41419-024-07137-z. https://pubmed.ncbi.nlm.nih.gov/39389957/
5. Rousseau, Vanessa, Einig, Elias, Jin, Chao, Flentje, Michael, Popov, Nikita. 2023. Trim33 masks a non-transcriptional function of E2f4 in replication fork progression. In Nature communications, 14, 5143. doi:10.1038/s41467-023-40847-0. https://pubmed.ncbi.nlm.nih.gov/37612308/
6. Shen, Xiangyi, Li, Xiaoguang, Wu, Tao, Hu, Xiaoyu, Wu, Li. 2024. TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription. In Cellular & molecular immunology, 21, 752-769. doi:10.1038/s41423-024-01179-1. https://pubmed.ncbi.nlm.nih.gov/38822080/
7. McAvera, Roisin M, Morgan, Jonathan J, Herrero, Ana B, Mills, Ken I, Crawford, Lisa J. 2024. TRIM33 loss in multiple myeloma is associated with genomic instability and sensitivity to PARP inhibitors. In Scientific reports, 14, 8797. doi:10.1038/s41598-024-58828-8. https://pubmed.ncbi.nlm.nih.gov/38627415/
8. Ji, Wenjing, Zhang, Weibin, Zhang, Xin, Ke, Yue. 2024. TRIM33 enhances the ubiquitination of TFRC to enhance the susceptibility of liver cancer cells to ferroptosis. In Cellular signalling, 121, 111268. doi:10.1016/j.cellsig.2024.111268. https://pubmed.ncbi.nlm.nih.gov/38909931/
9. Rajderkar, Sudha, Mann, Jeffrey M, Panaretos, Christopher, Ralston, Benjamin, Kaartinen, Vesa. 2019. Trim33 is required for appropriate development of pre-cardiogenic mesoderm. In Developmental biology, 450, 101-114. doi:10.1016/j.ydbio.2019.03.018. https://pubmed.ncbi.nlm.nih.gov/30940539/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest