C57BL/6JCya-Trim33em1flox/Cya
Common Name:
Trim33-flox
Product ID:
S-CKO-17229
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Trim33-flox
Strain ID
CKOCMP-94093-Trim33-B6J-VA
Gene Name
Product ID
S-CKO-17229
Gene Alias
8030451N04Rik; Ecto; Tif1g
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim33em1flox/Cya mice (Catalog S-CKO-17229) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029444
NCBI RefSeq
NM_053170
Target Region
Exon 2~4
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Trim33, also known as TIF-1γ, is a transcriptional repressor and E3 ubiquitin ligase. It is involved in multiple biological processes, including regulating signaling pathways like TGF-β/SMAD, and is crucial for maintaining genome stability. It plays roles in cell differentiation, development, and homeostasis, and its dysregulation is associated with various diseases [1,5,7]. Genetic mouse models, such as gene knockout (KO) and conditional knockout (CKO), have been instrumental in studying Trim33's functions.
In KO mouse models, Trim33 has been shown to have diverse roles. Hematopoietic-specific Trim33 knockout sensitized mice to bleomycin-induced pulmonary fibrosis, indicating its role as a negative regulator of lung fibrosis [1]. In dendritic cell (DC) development, conditional deletion of Trim33 in vivo caused rapid loss of DC progenitors, pDCs, and cDC1 subset, revealing its essential role in DC differentiation [2,6]. In prostate cancer, TRIM33 knockdown sensitized PCa cells to AR antagonists, suggesting its role as an oncogenic AR coactivator [3]. In esophageal squamous cell carcinoma, TRIM33 promotes tumor growth by regulating P53-related glycolysis [4]. Loss of Trim33 in multiple myeloma is associated with genomic instability and increased sensitivity to PARP inhibitors [7]. In hepatocellular carcinoma, TRIM33 promotes susceptibility to ferroptosis through ubiquitination of TFRC [8]. Epiblast-specific Trim33 mutant embryos had cardiac defects, highlighting its role in pre-cardiogenic mesoderm development [9].
In conclusion, Trim33 is a multifunctional protein involved in cell differentiation, disease development, and maintaining genome integrity. The KO/CKO mouse models have significantly contributed to understanding its roles in diseases like pulmonary fibrosis, cancer, and multiple myeloma, providing potential therapeutic targets for these conditions.
References:
1. Boutanquoi, Pierre-Marie, Burgy, Olivier, Beltramo, Guillaume, Goirand, Françoise, Bonniaud, Philippe. 2020. TRIM33 prevents pulmonary fibrosis by impairing TGF-β1 signalling. In The European respiratory journal, 55, . doi:10.1183/13993003.01346-2019. https://pubmed.ncbi.nlm.nih.gov/32184320/
2. Tiniakou, Ioanna, Hsu, Pei-Feng, Lopez-Zepeda, Lorena S, Mazzoni, Esteban O, Reizis, Boris. 2024. Genome-wide screening identifies Trim33 as an essential regulator of dendritic cell differentiation. In Science immunology, 9, eadi1023. doi:10.1126/sciimmunol.adi1023. https://pubmed.ncbi.nlm.nih.gov/38608038/
3. Chen, Mi, Lingadahalli, Shreyas, Narwade, Nitin, Poon, Terence Chuen Wai, Cheung, Edwin. 2022. TRIM33 drives prostate tumor growth by stabilizing androgen receptor from Skp2-mediated degradation. In EMBO reports, 23, e53468. doi:10.15252/embr.202153468. https://pubmed.ncbi.nlm.nih.gov/35785414/
4. Xia, Tian, Meng, Lian, Xu, Guixuan, Sun, Hao, Chen, Hao. 2024. TRIM33 promotes glycolysis through regulating P53 K48-linked ubiquitination to promote esophageal squamous cell carcinoma growth. In Cell death & disease, 15, 740. doi:10.1038/s41419-024-07137-z. https://pubmed.ncbi.nlm.nih.gov/39389957/
5. Rousseau, Vanessa, Einig, Elias, Jin, Chao, Flentje, Michael, Popov, Nikita. 2023. Trim33 masks a non-transcriptional function of E2f4 in replication fork progression. In Nature communications, 14, 5143. doi:10.1038/s41467-023-40847-0. https://pubmed.ncbi.nlm.nih.gov/37612308/
6. Shen, Xiangyi, Li, Xiaoguang, Wu, Tao, Hu, Xiaoyu, Wu, Li. 2024. TRIM33 plays a critical role in regulating dendritic cell differentiation and homeostasis by modulating Irf8 and Bcl2l11 transcription. In Cellular & molecular immunology, 21, 752-769. doi:10.1038/s41423-024-01179-1. https://pubmed.ncbi.nlm.nih.gov/38822080/
7. McAvera, Roisin M, Morgan, Jonathan J, Herrero, Ana B, Mills, Ken I, Crawford, Lisa J. 2024. TRIM33 loss in multiple myeloma is associated with genomic instability and sensitivity to PARP inhibitors. In Scientific reports, 14, 8797. doi:10.1038/s41598-024-58828-8. https://pubmed.ncbi.nlm.nih.gov/38627415/
8. Ji, Wenjing, Zhang, Weibin, Zhang, Xin, Ke, Yue. 2024. TRIM33 enhances the ubiquitination of TFRC to enhance the susceptibility of liver cancer cells to ferroptosis. In Cellular signalling, 121, 111268. doi:10.1016/j.cellsig.2024.111268. https://pubmed.ncbi.nlm.nih.gov/38909931/
9. Rajderkar, Sudha, Mann, Jeffrey M, Panaretos, Christopher, Ralston, Benjamin, Kaartinen, Vesa. 2019. Trim33 is required for appropriate development of pre-cardiogenic mesoderm. In Developmental biology, 450, 101-114. doi:10.1016/j.ydbio.2019.03.018. https://pubmed.ncbi.nlm.nih.gov/30940539/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen