C57BL/6JCya-Wwtr1em1flox/Cya
Common Name:
Wwtr1-flox
Product ID:
S-CKO-17278
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Wwtr1-flox
Strain ID
CKOCMP-97064-Wwtr1-B6J-VA
Gene Name
Product ID
S-CKO-17278
Gene Alias
2310058J06Rik; 2610021I22Rik; Taz
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wwtr1em1flox/Cya mice (Catalog S-CKO-17278) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029380
NCBI RefSeq
NM_133784
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Wwtr1, also known as TAZ (transcriptional coactivator with PDZ-binding motif), is a key transcriptional regulator. It is a core component of the Hippo signaling pathway, which is crucial for cell-cell interactions, growth, and tumor-suppression. Dysregulation of this pathway can lead to various diseases. Wwtr1 senses the cell microenvironment's structural and mechanical features and integrates signals from multiple upstream signaling pathways [2,3,4].
In non-alcoholic steatohepatitis (NASH), hepatocyte TAZ/Wwtr1 is significantly higher. Silencing hepatocyte TAZ in murine NASH models prevents or reverses hepatic inflammation, hepatocyte death, and fibrosis, but not steatosis. Hepatocyte-targeted TAZ expression in a steatosis model promotes NASH features, including fibrosis. Mechanistically, TAZ/TEAD-mediated induction of Indian hedgehog (Ihh) activates fibrogenic genes in hepatic stellate cells [1].
In epithelioid hemangioendothelioma (EHE), a TAZ-CAMTA1 gene fusion occurs in about 90% of cases. TAZ-CAMTA1 expression in endothelial cells drives EHE-like vascular tumors, and its inhibition leads to tumor regression. Activated TAZ resembles TAZ-CAMTA1 in this regard [5].
Mice deficient in Wwtr1 show clinical features of late-onset Fuchs' endothelial corneal dystrophy, such as reduced corneal endothelial cell density, abnormal cell morphology, and impaired wound healing [6].
In summary, Wwtr1 is a vital transcriptional regulator in the Hippo signaling pathway. Gene-knockout mouse models have revealed its role in diseases like NASH, EHE, and Fuchs' endothelial corneal dystrophy. These models help us understand the mechanisms underlying these diseases and may provide potential therapeutic targets.
References:
1. Wang, Xiaobo, Zheng, Ze, Caviglia, Jorge Matias, Schwabe, Robert F, Tabas, Ira. 2016. Hepatocyte TAZ/WWTR1 Promotes Inflammation and Fibrosis in Nonalcoholic Steatohepatitis. In Cell metabolism, 24, 848-862. doi:10.1016/j.cmet.2016.09.016. https://pubmed.ncbi.nlm.nih.gov/28068223/
2. Andl, Thomas, Zhou, Linli, Yang, Kun, Kadekaro, Ana Luisa, Zhang, Yuhang. 2017. YAP and WWTR1: New targets for skin cancer treatment. In Cancer letters, 396, 30-41. doi:10.1016/j.canlet.2017.03.001. https://pubmed.ncbi.nlm.nih.gov/28279717/
3. Zanconato, Francesca, Cordenonsi, Michelangelo, Piccolo, Stefano. . YAP/TAZ at the Roots of Cancer. In Cancer cell, 29, 783-803. doi:10.1016/j.ccell.2016.05.005. https://pubmed.ncbi.nlm.nih.gov/27300434/
4. Dupont, Sirio, Morsut, Leonardo, Aragona, Mariaceleste, Elvassore, Nicola, Piccolo, Stefano. 2011. Role of YAP/TAZ in mechanotransduction. In Nature, 474, 179-83. doi:10.1038/nature10137. https://pubmed.ncbi.nlm.nih.gov/21654799/
5. Driskill, Jordan H, Zheng, Yonggang, Wu, Bo-Kuan, Dellinger, Michael, Pan, Duojia. 2021. WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid hemangioendothelioma. In Genes & development, 35, 495-511. doi:10.1101/gad.348221.120. https://pubmed.ncbi.nlm.nih.gov/33766984/
6. Leonard, Brian C, Park, Sangwan, Kim, Soohyun, Raghunathan, Vijay Krishna, Thomasy, Sara M. . Mice Deficient in TAZ (Wwtr1) Demonstrate Clinical Features of Late-Onset Fuchs' Endothelial Corneal Dystrophy. In Investigative ophthalmology & visual science, 64, 22. doi:10.1167/iovs.64.4.22. https://pubmed.ncbi.nlm.nih.gov/37074694/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen