C57BL/6JCya-Khdc1bem1flox/Cya
Common Name:
Khdc1b-flox
Product ID:
S-CKO-17326
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Khdc1b-flox
Strain ID
CKOCMP-98582-Khdc1b-B6J-VA
Gene Name
Product ID
S-CKO-17326
Gene Alias
Khdc1; Khdc1c
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Khdc1bem1flox/Cya mice (Catalog S-CKO-17326) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000147615
NCBI RefSeq
NM_001113187
Target Region
Exon 1~3
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Khdc1b, a member of a small family of KH-domain containing proteins specific to eutherian mammals, is closely related to Khdc1a. It is specifically expressed in mouse oocytes and early embryos. KHDC1B may function as a translational regulator, potentially via its interaction with the mouse translational regulator cytoplasmic polyadenylation binding protein 1 (mCPEB1), to regulate the translation of mRNA targets required for oocyte maturation [1].
In Xenopus embryos, expression of KHDC1B led to cleavage arrest, a phenotype that was rescued by co-expression of mCPEB1. Co-immunoprecipitation and co-immunostaining experiments confirmed the functional interaction between KHDC1B and mCPEB1. Also, KHDC1B levels and its binding partners were shown to fluctuate with the cell cycle [1].
In conclusion, KHDC1B is a key translational regulator in oocyte maturation. The study of KHDC1B in model organisms like Xenopus embryos has provided insights into its function in regulating early embryonic development, potentially offering a better understanding of the molecular mechanisms underlying human infertility and ovarian germ cell tumors.
References:
1. Cai, Congli, Tamai, Keiko, Molyneaux, Kathleen. 2010. KHDC1B is a novel CPEB binding partner specifically expressed in mouse oocytes and early embryos. In Molecular biology of the cell, 21, 3137-48. doi:10.1091/mbc.E10-03-0255. https://pubmed.ncbi.nlm.nih.gov/20668163/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen