C57BL/6NCya-Hnrnpfem1flox/Cya
Common Name:
Hnrnpf-flox
Product ID:
S-CKO-17335
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hnrnpf-flox
Strain ID
CKOCMP-98758-Hnrnpf-B6N-VA
Gene Name
Product ID
S-CKO-17335
Gene Alias
4833420I20Rik; Hnrpf
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Hnrnpfem1flox/Cya mice (Catalog S-CKO-17335) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000167182
NCBI RefSeq
NM_001166432
Target Region
Exon 4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Hnrnpf, a member of the HNRNPF/H family of heterogeneous nuclear RNA proteins, is a critical regulator of RNA maturation. It is involved in regulating splicing, especially alternative splicing, 5' capping, 3' polyadenylation of RNAs, and RNA export. Hnrnpf typically binds RNA sequences rich in guanine (G) residues, which can potentially form G-quadruplex structures [1].
In gastric cancer, the VAX2-LINC01189-hnRNPF signaling axis regulates cell invasion and migration. VAX2, as an oncogene, represses LINC01189 transcription. LINC01189 binds to hnRNPF to enhance its degradation through ubiquitination, thus influencing GC cell invasion and migration [2].
In male infertility, overexpressing the hnrnpH/F-orthologue Glorund in Drosophila led to low-quality semen production, suggesting that dysregulation of hnrnpH/F may cause subfertility or infertility in men [3].
In myocardial infarction, circCacna1c can inhibit the Hnrnpf/RIPK1-mediated cardiomyocyte necroptosis, improving cardiac function. CircCacna1c interacts with Hnrnpf in the cytoplasm, preventing its nuclear translocation and reducing Hnrnpf-dependent RIPK1 expression [4].
In conclusion, Hnrnpf is essential for RNA maturation processes. Model-based research, such as Drosophila overexpression and in-vivo studies in mouse models for diseases like gastric cancer, male infertility, and myocardial infarction, has revealed its role in various biological processes and disease conditions. These findings provide insights into potential therapeutic targets for related diseases.
References:
1. Brownmiller, Tayvia, Caplen, Natasha J. 2023. The HNRNPF/H RNA binding proteins and disease. In Wiley interdisciplinary reviews. RNA, 14, e1788. doi:10.1002/wrna.1788. https://pubmed.ncbi.nlm.nih.gov/37042074/
2. Hong, Linjie, Yang, Ping, Zhang, Luyu, Xiang, Li, Wang, Jide. 2023. The VAX2-LINC01189-hnRNPF signaling axis regulates cell invasion and migration in gastric cancer. In Cell death discovery, 9, 387. doi:10.1038/s41420-023-01688-4. https://pubmed.ncbi.nlm.nih.gov/37865686/
3. Netherton, Jacob K, Ogle, Rachel A, Robinson, Benjamin R, Hime, Gary R, Baker, Mark A. 2024. The role of HnrnpF/H as a driver of oligoteratozoospermia. In iScience, 27, 110198. doi:10.1016/j.isci.2024.110198. https://pubmed.ncbi.nlm.nih.gov/39092172/
4. Jia, Yi, Yuan, Xiaosu, Feng, Luxin, Ao, Xiang, Wang, Jianxun. 2024. m6A-modified circCacna1c regulates necroptosis and ischemic myocardial injury by inhibiting Hnrnpf entry into the nucleus. In Cellular & molecular biology letters, 29, 140. doi:10.1186/s11658-024-00649-8. https://pubmed.ncbi.nlm.nih.gov/39533214/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen