C57BL/6JCya-Kdm1aem1flox/Cya
Common Name:
Kdm1a-flox
Product ID:
S-CKO-17391
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kdm1a-flox
Strain ID
CKOCMP-99982-Kdm1a-B6J-VA
Gene Name
Product ID
S-CKO-17391
Gene Alias
1810043O07Rik; Aof2; D4Ertd478e; Kdm1; Lsd1; mKIAA0601
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kdm1aem1flox/Cya mice (Catalog S-CKO-17391) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105847
NCBI RefSeq
NM_001347221
Target Region
Exon 5~6
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Kdm1a, also known as LSD1 (lysine-specific demethylase 1), is a histone demethylase. It contains a flavin adenine dinucleotide (FAD)-dependent amine oxidase domain and demethylates histone 3 lysine 4 and histone 3 lysine 9 (H3K4me1/2 and H3K9me1/2). It has emerged as an epigenetic developmental regulator and is involved in multiple biological processes, and its dysregulation is associated with carcinogenesis [5].
Kdm1a promotes the progression of various cancers. In thyroid cancer, it maintains cancer stem cell stemness through the Wnt/β-catenin signaling pathway by down-regulating APC2 and DKK1, antagonists of the canonical Wnt pathway [1]. In acute myeloid leukemia (AML), it is a key regulator of stem cell potential, and its inhibitor ORY-1001 can induce blast differentiation and reduce leukemic stem cell capacity [2]. In gastric cancer, inhibition of Kdm1A causes organoid growth retardation, and its cancer-supporting functions center on repression of NDRG1, whose de-repression leads to inhibition of Wnt signaling and G1 cell cycle arrest [3]. In breast cancer, KDM1A acts as a transcriptional activator of PIAS4, which facilitates the SUMOylation of SLC7A11, and Tanshinone IIA can promote ferroptosis by suppressing the KDM1A/PIAS4/SLC7A11 axis [4]. In ovarian cancer, KDM1A is highly overexpressed, and its knockdown or inhibition sensitizes cancer cells to chemotherapy drugs, reducing cell viability, clonogenic survival, and inducing apoptosis [6].
In conclusion, Kdm1a is an important epigenetic regulator involved in cancer development and progression. Studies using loss-of-function models have revealed its role in multiple cancer-related biological processes, highlighting its potential as a therapeutic target for cancer treatment.
References:
1. Zhang, Wei, Ruan, Xianhui, Li, Yaoshuang, Zheng, Xiangqian, Gao, Ming. 2022. KDM1A promotes thyroid cancer progression and maintains stemness through the Wnt/β-catenin signaling pathway. In Theranostics, 12, 1500-1517. doi:10.7150/thno.66142. https://pubmed.ncbi.nlm.nih.gov/35198054/
2. Maes, Tamara, Mascaró, Cristina, Tirapu, Iñigo, Castro-Palomino, Julio Cesar, Buesa, Carlos. 2018. ORY-1001, a Potent and Selective Covalent KDM1A Inhibitor, for the Treatment of Acute Leukemia. In Cancer cell, 33, 495-511.e12. doi:10.1016/j.ccell.2018.02.002. https://pubmed.ncbi.nlm.nih.gov/29502954/
3. Mircetic, Jovan, Camgöz, Aylin, Abohawya, Moustafa, Buchholz, Frank, Stange, Daniel E. 2023. Nuclease technology Screen in Gastric Cancer Patient-Derived Organoids Reveals KDM1A-NDRG1 Axis as a Targetable Vulnerability. In Small methods, 7, e2201605. doi:10.1002/smtd.202201605. https://pubmed.ncbi.nlm.nih.gov/36908010/
4. Luo, Na, Zhang, KeJing, Li, Xin, Hu, Yu, Guo, Lei. 2024. Tanshinone IIA destabilizes SLC7A11 by regulating PIAS4-mediated SUMOylation of SLC7A11 through KDM1A, and promotes ferroptosis in breast cancer. In Journal of advanced research, 69, 313-327. doi:10.1016/j.jare.2024.04.009. https://pubmed.ncbi.nlm.nih.gov/38615741/
5. Ismail, Tayaba, Lee, Hyun-Kyung, Kim, Chowon, Park, Tae Joo, Lee, Hyun-Shik. 2018. KDM1A microenvironment, its oncogenic potential, and therapeutic significance. In Epigenetics & chromatin, 11, 33. doi:10.1186/s13072-018-0203-3. https://pubmed.ncbi.nlm.nih.gov/29921310/
6. Chen, Yihong, Johnson, Jessica D, Jayamohan, Sridharan, Kost, Edward, Sareddy, Gangadhara R. 2024. KDM1A/LSD1 inhibition enhances chemotherapy response in ovarian cancer. In Molecular carcinogenesis, 63, 2026-2039. doi:10.1002/mc.23792. https://pubmed.ncbi.nlm.nih.gov/38990091/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen