C57BL/6JCya-Vcam1em1flox/Cya
Common Name:
Vcam1-flox
Product ID:
S-CKO-17424
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Vcam1-flox
Strain ID
CKOCMP-22329-Vcam1-B6J-VA
Gene Name
Product ID
S-CKO-17424
Gene Alias
CD106; Vcam-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Vcam1em1flox/Cya mice (Catalog S-CKO-17424) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029574
NCBI RefSeq
NM_011693
Target Region
Exon 1
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Vcam1, known as Vascular cell adhesion molecule 1, belongs to the immunoglobulin superfamily of cell adhesion molecules. It plays a crucial role in mediating the strong adherence of leukocytes to endothelial cells, regulating inflammation, and facilitating leukocyte migration. It is involved in multiple pathways such as the AKT-mTOR-CXCL1 axis, and is of great biological importance in processes like immune response, cancer progression, and maintaining vascular integrity. Genetic models, like KO/CKO mouse models, are valuable tools for studying its functions.
In gastric cancer, H3K18 lactylation upregulates Vcam1 transcription, which activates AKT-mTOR signaling, promoting tumor cell proliferation, EMT transition, and metastasis. Vcam1 also upregulates CXCL1 expression via the AKT-mTOR pathway, facilitating the recruitment of hGC-MSCs and M2 macrophages [1]. In ovarian cancer, the VLA4/VCAM1 pathway in M2 macrophages is involved in regulating vascular permeability and ascites development. Targeting this axis can enhance vascular integrity and prevent ascites formation [2]. In sickle cell anemia, certain VCAM1 promoter haplotypes lead to higher VCAM1 expression, affecting systemic vasculopathy risk [3]. In liver fibrosis, VCAM1 expressed by liver sinusoidal endothelial cells promotes LSEC capillarization and liver fibrosis [4]. In chronic cerebral hypoperfusion, increased VCAM1 expression on brain endothelial cells drives blood-brain barrier impairment, and blocking VCAM1-mediated BBB impairment may be a new treatment strategy [5].
In conclusion, Vcam1 is essential in regulating inflammation, immune responses, and is closely associated with the progression of various diseases including cancer, ovarian ascites, sickle cell anemia-related vasculopathy, liver fibrosis, and chronic cerebral hypoperfusion-related neurodegenerative diseases. Studies using KO/CKO mouse models have significantly contributed to understanding its role in these disease conditions, providing potential therapeutic targets for treatment.
References:
1. Zhao, Yupeng, Jiang, Jiang, Zhou, Peng, Li, Ranran, Xia, Jiazeng. 2024. H3K18 lactylation-mediated VCAM1 expression promotes gastric cancer progression and metastasis via AKT-mTOR-CXCL1 axis. In Biochemical pharmacology, 222, 116120. doi:10.1016/j.bcp.2024.116120. https://pubmed.ncbi.nlm.nih.gov/38461905/
2. Zhang, Shibo, Xie, Bingfan, Wang, Lijie, Liu, Changqing, He, Huanhuan. . Macrophage-mediated vascular permeability via VLA4/VCAM1 pathway dictates ascites development in ovarian cancer. In The Journal of clinical investigation, 131, . doi:10.1172/JCI140315. https://pubmed.ncbi.nlm.nih.gov/33295887/
3. Silva, Marisa, Coelho, Andreia, Vargas, Sofia, Faustino, Paula. 2021. VCAM1, HMOX1 and NOS3 differential endothelial expression may impact sickle cell anemia vasculopathy. In Blood cells, molecules & diseases, 93, 102639. doi:10.1016/j.bcmd.2021.102639. https://pubmed.ncbi.nlm.nih.gov/34999313/
4. Guo, Qianqian, Furuta, Kunimaro, Islam, Shahidul, Hirsova, Petra, Ibrahim, Samar H. 2022. Liver sinusoidal endothelial cell expressed vascular cell adhesion molecule 1 promotes liver fibrosis. In Frontiers in immunology, 13, 983255. doi:10.3389/fimmu.2022.983255. https://pubmed.ncbi.nlm.nih.gov/36091042/
5. Zhang, Huiwen, Shang, Junkui, Li, Wei, Gao, Dandan, Zhang, Jiewen. 2024. Increased Expression of VCAM1 on Brain Endothelial Cells Drives Blood-Brain Barrier Impairment Following Chronic Cerebral Hypoperfusion. In ACS chemical neuroscience, 15, 2028-2041. doi:10.1021/acschemneuro.4c00039. https://pubmed.ncbi.nlm.nih.gov/38710594/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen