C57BL/6JCya-Gpx1em1flox/Cya
Common Name:
Gpx1-flox
Product ID:
S-CKO-17425
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpx1-flox
Strain ID
CKOCMP-14775-Gpx1-B6J-VA
Gene Name
Product ID
S-CKO-17425
Gene Alias
CGPx; GPx-1; GSHPx-1; Gpx
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpx1em1flox/Cya mice (Catalog S-CKO-17425) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000082429
NCBI RefSeq
NM_008160
Target Region
Exon 1~2
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Glutathione peroxidase 1 (GPX1), the first identified selenoprotein, is a widely expressed antioxidant enzyme. It uses glutathione as a substrate to catalyze hydrogen peroxide, lipid peroxide, and peroxynitrite, reducing intracellular oxidative stress. The GPX1 gene is regulated at transcriptional, post-transcriptional, and translational levels and participates in multiple signaling pathways, influencing cell proliferation, apoptosis, invasion, immune response, and chemoresistance [2].
In cancer research, high GPX1 expression is associated with poor survival in acute myeloid leukemia (AML) patients, linking to immunosuppression. GPX1 expression positively correlates with myeloid-derived suppressor cells (MDSCs), monocytes, T-cell exhaustion, MDSC-promoting CCR2, and immune inhibitory checkpoints, while negatively with CD4+ and CD8+ T cells. Silencing GPX1 reduces AML cell viability and CCR2 expression [1].
In laryngeal squamous cell carcinoma (LSCC), high GPX1 expression is correlated with lymph node metastasis, TNM stage, and worse prognosis, and is an independent prognostic factor [3].
In brain lower-grade glioma (LGG), GPX1 overexpression is significantly correlated with poor prognosis, and is an independent predictive factor. High GPX1 expression links to a shorter overall survival (OS), and the interaction between GPX1 and chemoradiotherapy exhibits a beneficial clinical effect. GPX1 is also negatively correlated with the half-inhibition concentration (IC50) of temozolomide (TMZ) [4].
In kidney cancer, high GPX1 levels are positively correlated with short OS, distant metastasis, lymphatic metastasis, and tumor stage, and GPX1 may serve as a diagnostic and prognostic biomarker [7]. Additionally, the GPX1 Pro198Leu polymorphism is associated with a significantly increased risk of bladder cancer [8].
In the context of aging, GPX1 knockout (GPX1-/-) in mice causes accelerated age-related cataracts, indicating that GPX1 is more critical than catalase for lens transparency, optical quality, and homeostasis in the aging lens [5].
In Crohn's disease, monocyte-derived macrophages from individuals with active disease have increased susceptibility to H2O2-induced ferroptosis cell death, potentially due to reduced GPX1 expression [6].
In conclusion, GPX1 is a crucial antioxidant enzyme with significant roles in various biological processes and disease conditions. Studies using gene knockout models, especially in cancer, aging-related cataracts, and Crohn's disease, have revealed its importance in disease development, prognosis, and potential as a therapeutic target.
References：
1. Zhang, Jian, Peng, Yuhui, He, Yan, Guo, Penxiang, Zhang, Qifang. 2021. GPX1-associated prognostic signature predicts poor survival in patients with acute myeloid leukemia and involves in immunosuppression. In Biochimica et biophysica acta. Molecular basis of disease, 1868, 166268. doi:10.1016/j.bbadis.2021.166268. https://pubmed.ncbi.nlm.nih.gov/34536536/
2. Zhao, Yangjing, Wang, Hui, Zhou, Jingdong, Shao, Qixiang. 2022. Glutathione Peroxidase GPX1 and Its Dichotomous Roles in Cancer. In Cancers, 14, . doi:10.3390/cancers14102560. https://pubmed.ncbi.nlm.nih.gov/35626163/
3. Zhang, Qicheng, Xu, Hongli, You, Yiwen, Zhang, Jie, Chen, Renjie. 2017. High Gpx1 expression predicts poor survival in laryngeal squamous cell carcinoma. In Auris, nasus, larynx, 45, 13-19. doi:10.1016/j.anl.2017.05.012. https://pubmed.ncbi.nlm.nih.gov/28641905/
4. Chen, Xueqin, Fu, Guotao, Li, Linglan, Ke, Zunhua, Zhang, Rongqiang. 2022. Selenoprotein GPX1 is a prognostic and chemotherapy-related biomarker for brain lower grade glioma. In Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 74, 127082. doi:10.1016/j.jtemb.2022.127082. https://pubmed.ncbi.nlm.nih.gov/36155420/
5. Varadaraj, Kulandaiappan, Gao, Junyuan, Mathias, Richard T, Kumari, S Sindhu. 2022. GPX1 knockout, not catalase knockout, causes accelerated abnormal optical aberrations and cataract in the aging lens. In Molecular vision, 28, 11-20. doi:. https://pubmed.ncbi.nlm.nih.gov/35400989/
6. Sousa, James A, Callejas, Blanca E, Wang, Arthur, Raman, Maitreyi, McKay, Derek M. 2024. GPx1 deficiency confers increased susceptibility to ferroptosis in macrophages from individuals with active Crohn's disease. In Cell death & disease, 15, 903. doi:10.1038/s41419-024-07289-y. https://pubmed.ncbi.nlm.nih.gov/39695083/
7. Cheng, Yongbiao, Xu, Tianbo, Li, Sen, Ruan, Hailong. 2019. GPX1, a biomarker for the diagnosis and prognosis of kidney cancer, promotes the progression of kidney cancer. In Aging, 11, 12165-12176. doi:10.18632/aging.102555. https://pubmed.ncbi.nlm.nih.gov/31844035/
8. Cao, Ming, Mu, Xin, Jiang, Chen, Chen, Haige, Xue, Wei. 2013. Single-nucleotide polymorphisms of GPX1 and MnSOD and susceptibility to bladder cancer: a systematic review and meta-analysis. In Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35, 759-64. doi:10.1007/s13277-013-1103-6. https://pubmed.ncbi.nlm.nih.gov/24037914/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen