C57BL/6JCya-Lrrc8aem1flox/Cya
Common Name:
Lrrc8a-flox
Product ID:
S-CKO-17452
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lrrc8a-flox
Strain ID
CKOCMP-241296-Lrrc8a-B6J-VA
Gene Name
Product ID
S-CKO-17452
Gene Alias
Lrrc8; SWELL1; ebo; mKIAA1437
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lrrc8aem1flox/Cya mice (Catalog S-CKO-17452) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095078
NCBI RefSeq
NM_177725
Target Region
Exon 3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Lrrc8a, also known as Swell 1, is a core component of volume-regulated anion channels (VRACs) [5]. VRACs are activated by hypotonic conditions and play a crucial role in the regulatory volume decrease process. This gene is involved in maintaining cellular stability, regulating immune cell activation, and is associated with multiple biological processes and disease-related pathways [2]. Genetic models, such as gene knockout (KO) and conditional knockout (CKO) mouse models, are valuable for studying its functions.
In T cells, deletion of Lrrc8a impairs T cell activation and function, especially under weak TCR stimulation, indicating that Lrrc8a-controlled cell volume regulation is required for optimal T cell activation [1].
In allergic rhinitis, knocking down Lrrc8a in a mouse model reduces eosinophil infiltration, down-regulates the expression of NADPH oxidase-related factors and Th2 inflammatory markers, and decreases NF-κB pathway activation, suggesting Lrrc8a may be a potential target for treatment [2].
In intracerebral hemorrhagic stroke mouse models, conditional knockout of Lrrc8a in microglia/macrophage accelerates hematoma clearance, reduces neuronal death, and improves functional recovery by promoting phagocytosis through the AMPK-Nrf2-CD36 pathway [3].
In myocardial infarction mouse models, myofibroblast-specific knockout of Lrrc8a attenuates myofibroblast transformation, fibrotic remodeling, and ventricular dysfunction, revealing its role in cardiac fibrosis regulation [4].
In conclusion, Lrrc8a is essential for regulating cell volume, immune cell function, and inflammation. KO/CKO mouse models have demonstrated its significance in diseases such as T cell-related immune responses, allergic rhinitis, intracerebral hemorrhagic stroke, and myocardial infarction-induced cardiac fibrosis. These findings highlight Lrrc8a as a potential therapeutic target in these disease areas.
References:
1. Wang, Yuman, Sun, Zaiqiao, Ping, Jieming, Yin, Lei, Wu, Ning. 2023. Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function. In Nature communications, 14, 7075. doi:10.1038/s41467-023-42817-y. https://pubmed.ncbi.nlm.nih.gov/37925509/
2. Meng, Linghui, Hao, Dingqian, Liu, Yuan, Liu, Shengyang, Shi, Li. 2024. LRRC8A drives NADPH oxidase-mediated mitochondrial dysfunction and inflammation in allergic rhinitis. In Journal of translational medicine, 22, 1034. doi:10.1186/s12967-024-05853-w. https://pubmed.ncbi.nlm.nih.gov/39550567/
3. Liu, Jing, Shen, Danmin, Wei, Chao, Yang, Fei, Li, Qian. 2022. Inhibition of the LRRC8A channel promotes microglia/macrophage phagocytosis and improves outcomes after intracerebral hemorrhagic stroke. In iScience, 25, 105527. doi:10.1016/j.isci.2022.105527. https://pubmed.ncbi.nlm.nih.gov/36465125/
4. Chen, Xiyao, Zhang, Fuyang, Hu, Guangyu, Wang, Ning, Wang, Xiaoming. 2022. LRRC8A critically regulates myofibroblast phenotypes and fibrotic remodeling following myocardial infarction. In Theranostics, 12, 5824-5835. doi:10.7150/thno.75200. https://pubmed.ncbi.nlm.nih.gov/35966575/
5. Kunzelmann, Karl. 2015. TMEM16, LRRC8A, bestrophin: chloride channels controlled by Ca(2+) and cell volume. In Trends in biochemical sciences, 40, 535-43. doi:10.1016/j.tibs.2015.07.005. https://pubmed.ncbi.nlm.nih.gov/26254230/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen