C57BL/6JCya-Il1rl1em1flox/Cya
Common Name:
Il1rl1-flox
Product ID:
S-CKO-17463
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Il1rl1-flox
Strain ID
CKOCMP-17082-Il1rl1-B6J-VB
Gene Name
Product ID
S-CKO-17463
Gene Alias
DER4; Fit-1; Ly84; ST2L; St2; St2-rs1; T1; T1/ST2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Il1rl1em1flox/Cya mice (Catalog S-CKO-17463) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000097772
NCBI RefSeq
NM_001025602
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Il1rl1, also known as ST2, encodes the ST2 receptor for IL-33, an important cytokine in inflammation regulation. The IL-33/Il1rl1 axis is involved in multiple biological processes and disease-related pathways, playing a crucial role in immune responses and cell survival regulation [2,3].
In acute myeloid leukemia (AML), using a Cbfb-MYH11 knock-in mouse model, it was shown that Il1rl1 is dynamically expressed on Cbfb-MYH11+ leukemia stem cells. Treatment with IL-33, the ligand of Il1rl1, increased serial replating ability and expression of pro-survival proteins in vitro, and Il1rl1+ cells survived chemotherapy better in vivo [2]. Further study demonstrated that the IL-33/Il1rl1 axis in AML cells decreased drug sensitivity, increased the expression of IL-4 and IL-6, activated p38 MAPK and NF-κB, decreased apoptosis, and the MAPK inhibitor could reverse these effects [4]. In the tumor microenvironment, Il1rl1 signaling in regulatory T (Treg) cells dampened the antitumor activity of IL-33 and PD-1 blockade. The amphiregulin (AREG)-epidermal growth factor receptor (EGFR) axis mediated cross-talk between Il1rl1+ Treg cells and cancer-associated fibroblasts (CAFs), and AREG mAbs and IL-33 could concertedly inhibit tumor growth [1].
In conclusion, Il1rl1, through its interaction with IL-33, is crucial in regulating cell survival in AML and plays a significant role in the tumor microenvironment, influencing antitumor immunity. Mouse models, such as the Cbfb-MYH11 knock-in mouse model, have been instrumental in revealing these functions, providing valuable insights into the pathogenesis of leukemia and cancer immunotherapy [1,2,4].
References:
1. Sun, Runzi, Zhao, Hongyu, Gao, David Shihong, Chen, Kong, Lu, Binfeng. 2023. Amphiregulin couples IL1RL1+ regulatory T cells and cancer-associated fibroblasts to impede antitumor immunity. In Science advances, 9, eadd7399. doi:10.1126/sciadv.add7399. https://pubmed.ncbi.nlm.nih.gov/37611111/
2. Wang, Yiqian, Richter, Lisa, Becker, Michelle, Amador, Catalina, Hyde, R Katherine. 2019. IL1RL1 is dynamically expressed on Cbfb-MYH11+ leukemia stem cells and promotes cell survival. In Scientific reports, 9, 1729. doi:10.1038/s41598-018-38408-3. https://pubmed.ncbi.nlm.nih.gov/30742053/
3. Cayrol, Corinne, Girard, Jean-Philippe. . Interleukin-33 (IL-33): A nuclear cytokine from the IL-1 family. In Immunological reviews, 281, 154-168. doi:10.1111/imr.12619. https://pubmed.ncbi.nlm.nih.gov/29247993/
4. Wang, Yiqian, Luo, Huanmin, Wei, Mengyi, Hyde, R Katherine, Gong, Qing. 2020. IL-33/IL1RL1 axis regulates cell survival through the p38 MAPK pathway in acute myeloid leukemia. In Leukemia research, 96, 106409. doi:10.1016/j.leukres.2020.106409. https://pubmed.ncbi.nlm.nih.gov/32652328/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen