C57BL/6JCya-Slamf1em1flox/Cya
Common Name:
Slamf1-flox
Product ID:
S-CKO-17467
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slamf1-flox
Strain ID
CKOCMP-27218-Slamf1-B6J-VA
Gene Name
Product ID
S-CKO-17467
Gene Alias
4933415F16; CD150; CDw150; ESTM51; IPO-3; Slam
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slamf1em1flox/Cya mice (Catalog S-CKO-17467) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015460
NCBI RefSeq
NM_013730
Target Region
Exon 2~3
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
SLAMF1, also known as CD150, is the founder of the signaling lymphocyte activation molecule (SLAM) family of cell-surface receptors. It is involved in regulating immune responses, as homotypic SLAM receptor-ligand interactions between myeloid and lymphoid cells are crucial for innate and adaptive immunity. It also participates in various cell-signaling pathways, such as TLR4-mediated TRAM-TRIF-dependent signaling in human macrophages [8].
In terms of disease-related findings, in hematologic malignancies, its differential expression among various types allows it to be considered as a diagnostical and potential prognostic marker, and it may also be a target for antibody-based or measles virus oncolytic therapy. In Hodgkin's lymphoma and chronic lymphocytic leukemia, it is involved in regulating malignant cell fate decision and tumor microenvironment [1].
In rheumatoid arthritis-associated interstitial lung disease (RA -ILD), SLAMF1 was identified as a mediator accounting for 4.693% of the mediating effect on the causal relationship between RA and ILD, and it is overexpressed in patients with lung fibrosis, correlating with a poor prognosis [2].
In non-alcoholic fatty liver disease (NAFLD), SLAMF1 levels are increased in NASH plasma samples, and its downregulation in HepG2 cells improves cell viability, suggesting it could be a biomarker and therapeutic target for NASH [3].
In choriocarcinoma, SLAMF1 promotes methotrexate resistance via activating autophagy, and targeting it may sensitize cells to MTX-based regimens [4].
In EBV-infected B cell tumors, although not a strong survival factor, SLAMF1 is necessary for cell survival in unfavorable growth conditions through the AKT signaling pathway [5].
In tuberculosis, SLAMF1 promotes Th1-protective responses, enhances the uptake of Mycobacterium tuberculosis by macrophages, and is involved in endolysosomal maturation [6].
In colorectal cancer, higher levels of SLAMF1+ innate lymphoid cells are observed in the blood of patients, and the SLAMF1-high group has a higher survival rate, suggesting it is an anti-tumor biomarker [7].
In conclusion, SLAMF1 plays diverse and significant roles in multiple biological processes and disease conditions. Its functions range from regulating immune responses to being implicated in the pathogenesis of various diseases such as hematologic malignancies, RA -ILD, NAFLD, choriocarcinoma, EBV-infected B cell tumors, tuberculosis, and colorectal cancer. The study of SLAMF1 using various models helps in understanding the underlying disease mechanisms and may provide potential therapeutic targets.
References:
1. Gordiienko, Inna, Shlapatska, Larysa, Kovalevska, Larysa, Sidorenko, Svetlana P. 2018. SLAMF1/CD150 in hematologic malignancies: Silent marker or active player? In Clinical immunology (Orlando, Fla.), 204, 14-22. doi:10.1016/j.clim.2018.10.015. https://pubmed.ncbi.nlm.nih.gov/30616923/
2. Liu, Muqiu, Jiang, Zhihao, Liu, Min, Du, Qun, Dong, Yan. 2024. SLAMF1 as a novel molecule mediating the causal association between rheumatoid arthritis and interstitial lung disease: A Mendelian randomization study combined with transcriptomics and in vivo validation. In International immunopharmacology, 142, 113082. doi:10.1016/j.intimp.2024.113082. https://pubmed.ncbi.nlm.nih.gov/39260308/
3. Gomez-Torres, Oscar, Amatya, Shripa, Kamberov, Lilly, Chapa-Rodriguez, Adrian, Cruz-Topete, Diana. 2022. SLAMF1 is expressed and secreted by hepatocytes and the liver in nonalcoholic fatty liver disease. In American journal of physiology. Gastrointestinal and liver physiology, 323, G177-G187. doi:10.1152/ajpgi.00289.2021. https://pubmed.ncbi.nlm.nih.gov/35853010/
4. Shi, Dazun, Zhang, Yu, Tian, Yan. 2020. SLAMF1 Promotes Methotrexate Resistance via Activating Autophagy in Choriocarcinoma Cells. In Cancer management and research, 12, 13427-13436. doi:10.2147/CMAR.S278012. https://pubmed.ncbi.nlm.nih.gov/33408515/
5. Yoon, Heejei, Kim, Eung Kweon, Ko, Young Hyeh. 2020. SLAMF1 contributes to cell survival through the AKT signaling pathway in Farage cells. In PloS one, 15, e0238791. doi:10.1371/journal.pone.0238791. https://pubmed.ncbi.nlm.nih.gov/32886706/
6. Barbero, Angela María, Trotta, Aldana, Genoula, Melanie, Barrionuevo, Paula, Pasquinelli, Virginia. 2020. SLAMF1 signaling induces Mycobacterium tuberculosis uptake leading to endolysosomal maturation in human macrophages. In Journal of leukocyte biology, 109, 257-273. doi:10.1002/JLB.4MA0820-655RR. https://pubmed.ncbi.nlm.nih.gov/32991756/
7. Qi, Jingjing, Crinier, Adeline, Escalière, Bertrand, Vivier, Eric, Su, Bing. 2021. Single-cell transcriptomic landscape reveals tumor specific innate lymphoid cells associated with colorectal cancer progression. In Cell reports. Medicine, 2, 100353. doi:10.1016/j.xcrm.2021.100353. https://pubmed.ncbi.nlm.nih.gov/34467243/
8. Yurchenko, Maria, Skjesol, Astrid, Ryan, Liv, Husebye, Harald, Espevik, Terje. 2018. SLAMF1 is required for TLR4-mediated TRAM-TRIF-dependent signaling in human macrophages. In The Journal of cell biology, 217, 1411-1429. doi:10.1083/jcb.201707027. https://pubmed.ncbi.nlm.nih.gov/29440514/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen