C57BL/6JCya-Cmtr1em1flox/Cya
Common Name:
Cmtr1-flox
Product ID:
S-CKO-17474
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cmtr1-flox
Strain ID
CKOCMP-74157-Cmtr1-B6J-VA
Gene Name
Product ID
S-CKO-17474
Gene Alias
1300018I05Rik; Ftsjd2; MTr1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cmtr1em1flox/Cya mice (Catalog S-CKO-17474) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024816
NCBI RefSeq
NM_028791
Target Region
Exon 9
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Cmtr1, also called IFN-stimulated gene 95 kDa protein (ISG95), is an RNA cap methyltransferase. It methylates the first transcribed nucleotide of RNA polymerase II transcripts, creating part of the mammalian RNA cap structure. This methylation is involved in various gene-expression mechanisms, such as splicing, translation, and innate immune response evasion, and also plays a role in DNA replication and cell proliferation [2,3,4,5].
Loss-of-function studies have revealed its crucial roles. In colorectal cancer, knockdown of Cmtr1 significantly suppressed cell proliferation and tumorigenicity both in vitro and in vivo. Mechanistically, it inhibited RNAPII recruitment to the transcription start site of STAT3, suppressing STAT3 expression and activation. Additionally, Cmtr1 knockdown enhanced the efficacy of PD1 blockade immunotherapy via increased infiltration of CD8 + T cells into the tumor microenvironment [1]. In mouse models, loss of Cmtr1 leads to embryonic lethality, and conditional deletion in the germline causes infertility. Also, in Cmtr1 mutant adult mouse livers, there is chronic activation of the interferon pathway [6].
In conclusion, Cmtr1 is essential for various biological processes like embryonic development, fertility, and cell proliferation. Its role in diseases such as colorectal cancer has been highlighted through functional studies, especially those using gene-knockout models. These models have provided insights into its function in tumorigenesis and immune regulation, offering potential targets for therapeutic intervention.
References:
1. You, A-Bin, Yang, Hu, Lai, Chun-Ping, Ding, Nan, Ye, Feng. 2023. CMTR1 promotes colorectal cancer cell growth and immune evasion by transcriptionally regulating STAT3. In Cell death & disease, 14, 245. doi:10.1038/s41419-023-05767-3. https://pubmed.ncbi.nlm.nih.gov/37024465/
2. Liang, Shang, Almohammed, Rajaei, Cowling, Victoria H. . The RNA cap methyltransferases RNMT and CMTR1 co-ordinate gene expression during neural differentiation. In Biochemical Society transactions, 51, 1131-1141. doi:10.1042/BST20221154. https://pubmed.ncbi.nlm.nih.gov/37145036/
3. Lukoszek, Radoslaw, Inesta-Vaquera, Francisco, Brett, Natasha J M, Roberts, Edward W, Cowling, Victoria H. 2024. CK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection. In Cell reports, 43, 114405. doi:10.1016/j.celrep.2024.114405. https://pubmed.ncbi.nlm.nih.gov/38923463/
4. Liang, Shang, Silva, Joana C, Suska, Olga, Almohammed, Rajaei, Cowling, Victoria H. . CMTR1 is recruited to transcription start sites and promotes ribosomal protein and histone gene expression in embryonic stem cells. In Nucleic acids research, 50, 2905-2922. doi:10.1093/nar/gkac122. https://pubmed.ncbi.nlm.nih.gov/35212377/
5. Inesta-Vaquera, Francisco, Cowling, Victoria H. 2017. Regulation and function of CMTR1-dependent mRNA cap methylation. In Wiley interdisciplinary reviews. RNA, 8, . doi:10.1002/wrna.1450. https://pubmed.ncbi.nlm.nih.gov/28971629/
6. Dohnalkova, Michaela, Krasnykov, Kyrylo, Mendel, Mateusz, Homolka, David, Pillai, Ramesh S. 2023. Essential roles of RNA cap-proximal ribose methylation in mammalian embryonic development and fertility. In Cell reports, 42, 112786. doi:10.1016/j.celrep.2023.112786. https://pubmed.ncbi.nlm.nih.gov/37436893/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen