C57BL/6JCya-Fntbem1flox/Cya
Common Name:
Fntb-flox
Product ID:
S-CKO-17503
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fntb-flox
Strain ID
CKOCMP-110606-Fntb-B6J-VC
Gene Name
Product ID
S-CKO-17503
Gene Alias
2010013E13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fntbem1flox/Cya mice (Catalog S-CKO-17503) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041008
NCBI RefSeq
NM_145927
Target Region
Exon 1
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Fntb, the farnesyltransferase β -subunit, is a key component of farnesyltransferase (FTase). FTase catalyzes the post-translational addition of a farnesyl group to proteins containing a CAAX motif, enabling these proteins to interact with cellular membranes. This process is involved in multiple cellular signaling pathways, such as those related to Ras proteins, which are crucial for cell growth, differentiation, and survival [5,6].
In Treg cells, Treg-specific deletion of Fntb leads to the development of fatal autoimmunity, indicating its importance in eTreg cell accumulation and function. Fntb promotes eTreg cell maintenance by regulating mTORC1 activity and ICOS expression [2]. In cardiomyocytes, overexpression of Fntb induces hypertrophy, apoptosis, and autophagic cell death, suggesting its role in cardiac remodeling and potential implications in heart-related diseases [3]. In breast cancer, certain Fntb promoter polymorphisms are independent predictors of survival, especially in triple-negative breast cancer (TNBC), and can modulate Fntb's transcriptional activity [1]. In ovarian cancer, the Fntb promoter polymorphism rs11623866 may be a potential predictive biomarker for lonafarnib treatment, as the G allele is associated with increased Fntb promoter activity and an unfavourable effect of lonafarnib is limited to patients with the GG genotype [4].
In conclusion, Fntb is essential for protein prenylation, which has far-reaching effects on various biological processes. Studies using gene-knockout or related models have revealed its significance in diseases such as autoimmunity, cardiac diseases, and certain cancers. Understanding Fntb's functions provides insights into the underlying mechanisms of these diseases and may guide the development of more targeted therapeutic strategies.
References:
1. Bachmann, Hagen Sjard, Jung, Dominik, Link, Theresa, Vetter, Martina, Kuhlmann, Jan Dominik. 2022. FNTB Promoter Polymorphisms Are Independent Predictors of Survival in Patients with Triple Negative Breast Cancer. In Cancers, 14, . doi:10.3390/cancers14030468. https://pubmed.ncbi.nlm.nih.gov/35158735/
2. Su, Wei, Chapman, Nicole M, Wei, Jun, Vogel, Peter, Chi, Hongbo. 2020. Protein Prenylation Drives Discrete Signaling Programs for the Differentiation and Maintenance of Effector Treg Cells. In Cell metabolism, 32, 996-1011.e7. doi:10.1016/j.cmet.2020.10.022. https://pubmed.ncbi.nlm.nih.gov/33207246/
3. Ding, Jie, Chen, Yu X, Chen, Yan, Hu, Shen J, Guo, Xiaogang. 2020. Overexpression of FNTB and the activation of Ras induce hypertrophy and promote apoptosis and autophagic cell death in cardiomyocytes. In Journal of cellular and molecular medicine, 24, 8998-9011. doi:10.1111/jcmm.15533. https://pubmed.ncbi.nlm.nih.gov/32579303/
4. Bachmann, Hagen Sjard, Meier, Werner, du Bois, Andreas, Aminossadati, Behnaz, Wimberger, Pauline. 2015. The FNTB promoter polymorphism rs11623866 as a potential predictive biomarker for lonafarnib treatment of ovarian cancer patients. In British journal of clinical pharmacology, 80, 1139-48. doi:10.1111/bcp.12688. https://pubmed.ncbi.nlm.nih.gov/26033044/
5. Andres, D A, Milatovich, A, Ozçelik, T, Goldstein, J L, Francke, U. . cDNA cloning of the two subunits of human CAAX farnesyltransferase and chromosomal mapping of FNTA and FNTB loci and related sequences. In Genomics, 18, 105-12. doi:. https://pubmed.ncbi.nlm.nih.gov/8276393/
6. Verhasselt, Henning, Stelmach, Patrick, Domin, Marie, Manthey, Iris, Bachmann, Hagen S. 2022. Characterization of the promoter of the human farnesyltransferase beta subunit and the impact of the transcription factor OCT-1 on its expression. In Genomics, 114, 110314. doi:10.1016/j.ygeno.2022.110314. https://pubmed.ncbi.nlm.nih.gov/35167937/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen