C57BL/6JCya-Tex14em1flox/Cya
Common Name:
Tex14-flox
Product ID:
S-CKO-17506
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tex14-flox
Strain ID
CKOCMP-83560-Tex14-B6J-VC
Gene Name
Product ID
S-CKO-17506
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tex14em1flox/Cya mice (Catalog S-CKO-17506) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060835
NCBI RefSeq
NM_031386
Target Region
Exon 7~8
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Tex14, or testis-expressed 14, is a crucial protein involved in the formation and stabilization of intercellular bridges in germ cells. It plays a significant role in spermatogenesis and oogenesis, with its functions also extending to kinetochore-microtubule attachment and regulation of the spindle assembly checkpoint during mitosis [1,2,3]. It is also associated with processes like iron homeostasis [4]. Genetic models, especially knockout mouse models, have been instrumental in studying Tex14's functions.
In Tex14 homozygous mutant fetal ovaries, germ cells form fewer cysts with abnormal intercellular connections, leading to fragmentation and a reduced number of primary oocytes with abnormal cytoplasmic enrichment [1]. In male Tex14 -/- mice, spermatogenesis halts before the completion of the first meiotic division as intercellular bridges are absent, causing male sterility [5]. However, female Tex14 -null mice are fertile despite the absence of embryonic intercellular bridges, though there are fewer oocytes in neonatal ovaries [6].
In conclusion, Tex14 is essential for the formation of intercellular bridges in germ cells, which is crucial for spermatogenesis. The gene knockout mouse models have revealed its key role in male fertility and provided insights into the processes of oogenesis. These findings contribute to understanding the mechanisms underlying infertility and potentially other related reproductive disorders.
References:
1. Ikami, Kanako, Nuzhat, Nafisa, Abbott, Haley, Tanner, Heather, Lei, Lei. 2021. Altered germline cyst formation and oogenesis in Tex14 mutant mice. In Biology open, 10, . doi:10.1242/bio.058807. https://pubmed.ncbi.nlm.nih.gov/34156079/
2. Rezende-Melo, C A, Caldeira-Brant, A L, Drumond-Bock, A L, Meistrich, M L, Chiarini-Garcia, H. . Spermatogonial asynchrony in Tex14 mutant mice lacking intercellular bridges. In Reproduction (Cambridge, England), 160, 205-215. doi:10.1530/REP-20-0118. https://pubmed.ncbi.nlm.nih.gov/32438343/
3. Mondal, Gourish, Ohashi, Akihiro, Yang, Lin, Rowley, Matthew, Couch, Fergus J. . Tex14, a Plk1-regulated protein, is required for kinetochore-microtubule attachment and regulation of the spindle assembly checkpoint. In Molecular cell, 45, 680-95. doi:10.1016/j.molcel.2012.01.013. https://pubmed.ncbi.nlm.nih.gov/22405274/
4. Benyamin, Beben, Esko, Tonu, Ried, Janina S, Swinkels, Dorine W, Whitfield, John B. 2014. Novel loci affecting iron homeostasis and their effects in individuals at risk for hemochromatosis. In Nature communications, 5, 4926. doi:10.1038/ncomms5926. https://pubmed.ncbi.nlm.nih.gov/25352340/
5. Greenbaum, Michael P, Yan, Wei, Wu, Meng-Hsieh, Rajkovic, Aleksandar, Matzuk, Martin M. 2006. TEX14 is essential for intercellular bridges and fertility in male mice. In Proceedings of the National Academy of Sciences of the United States of America, 103, 4982-7. doi:. https://pubmed.ncbi.nlm.nih.gov/16549803/
6. Greenbaum, Michael P, Iwamori, Naoki, Agno, Julio E, Matzuk, Martin M. 2008. Mouse TEX14 is required for embryonic germ cell intercellular bridges but not female fertility. In Biology of reproduction, 80, 449-57. doi:10.1095/biolreprod.108.070649. https://pubmed.ncbi.nlm.nih.gov/19020301/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen