C57BL/6JCya-Cdk9em1flox/Cya
Common Name:
Cdk9-flox
Product ID:
S-CKO-17524
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Cdk9-flox
Strain ID
CKOCMP-107951-Cdk9-B6J-VB
Gene Name
Product ID
S-CKO-17524
Gene Alias
PITALRE
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdk9em1flox/Cya mice (Catalog S-CKO-17524) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009699
NCBI RefSeq
NM_130860
Target Region
Exon 3~6
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
CDK9, or cyclin dependent kinase 9, is a serine/threonine kinase and a key component of the P-TEFb complex. It phosphorylates RNA polymerase (RNAP) II and other transcription factors, regulating gene transcription elongation in numerous physiological processes such as development, differentiation, and cell fate responses [6,7]. Aberrations in its activity have been linked to various cancers, making it an attractive therapeutic target [2].
In hepatocellular carcinoma (HCC), inhibition of CDK9 blocks PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis, leading to mitochondrial dysfunction and cell death. A novel CDK9 inhibitor, oroxylin A (OA), shows strong therapeutic potential against HCC and can overcome drug resistance [1]. In cancer research in general, there is intensive development of small-molecule CDK9 inhibitors and new strategies like PROTACs [2]. Pharmacological perturbation using selective CDK9 inhibition or degradation reveals that kinase degradation can have distinct effects compared to inhibition [3]. The PP2A-Integrator-CDK9 axis fine-tunes transcription, and targeting this axis can be a therapeutic strategy in cancer [4]. In solid tumors, CDK9 overexpression correlates with cancer development, and many solid cancers depend on its activity for oncogenic signaling [5]. In multiple myeloma, CDK9 inhibitors can overcome resistance and prolong survival, and in acute myeloid leukemia, targeting the CDK9 pathway is an attractive approach [8,9].
In conclusion, CDK9 is essential for regulating transcriptional elongation in key biological processes. Its dysregulation is associated with multiple cancers. Studies using inhibitors and other functional perturbation methods in various cancer models, including in vivo-like patient-derived tumor xenograft (PDX) models in HCC [1], have shown its potential as a therapeutic target in cancer treatment. These findings provide insights into the biological functions of CDK9 and open up new avenues for developing effective cancer therapies.
References:
1. Yao, Jingyue, Wang, Jubo, Xu, Ye, Guo, Yongjian, Wei, Libin. 2021. CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinoma. In Autophagy, 18, 1879-1897. doi:10.1080/15548627.2021.2007027. https://pubmed.ncbi.nlm.nih.gov/34890308/
2. Huang, Zhi, Wang, Tianqi, Wang, Cheng, Fan, Yan. 2022. CDK9 inhibitors in cancer research. In RSC medicinal chemistry, 13, 688-710. doi:10.1039/d2md00040g. https://pubmed.ncbi.nlm.nih.gov/35814933/
3. Olson, Calla M, Jiang, Baishan, Erb, Michael A, Winter, Georg E, Gray, Nathanael S. 2017. Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation. In Nature chemical biology, 14, 163-170. doi:10.1038/nchembio.2538. https://pubmed.ncbi.nlm.nih.gov/29251720/
4. Vervoort, Stephin J, Welsh, Sarah A, Devlin, Jennifer R, Gardini, Alessandro, Johnstone, Ricky W. 2021. The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer. In Cell, 184, 3143-3162.e32. doi:10.1016/j.cell.2021.04.022. https://pubmed.ncbi.nlm.nih.gov/34004147/
5. Mo, Christiana, Wei, Ning, Li, Terence, Mohammadi, Mahshid, Kuang, Chaoyuan. 2024. CDK9 inhibitors for the treatment of solid tumors. In Biochemical pharmacology, 229, 116470. doi:10.1016/j.bcp.2024.116470. https://pubmed.ncbi.nlm.nih.gov/39127153/
6. Eyvazi, Shirin, Hejazi, Mohammad Saeid, Kahroba, Homan, Zamiri, Reza Eghdam, Tarhriz, Vahideh. . CDK9 as an Appealing Target for Therapeutic Interventions. In Current drug targets, 20, 453-464. doi:10.2174/1389450119666181026152221. https://pubmed.ncbi.nlm.nih.gov/30362418/
7. Bacon, Curtis W, D'Orso, Iván. 2018. CDK9: a signaling hub for transcriptional control. In Transcription, 10, 57-75. doi:10.1080/21541264.2018.1523668. https://pubmed.ncbi.nlm.nih.gov/30227759/
8. Borowczak, Jędrzej, Szczerbowski, Krzysztof, Ahmadi, Navid, Szylberg, Łukasz. 2022. CDK9 inhibitors in multiple myeloma: a review of progress and perspectives. In Medical oncology (Northwood, London, England), 39, 39. doi:10.1007/s12032-021-01636-1. https://pubmed.ncbi.nlm.nih.gov/35092513/
9. Boffo, Silvia, Damato, Angela, Alfano, Luigi, Giordano, Antonio. 2018. CDK9 inhibitors in acute myeloid leukemia. In Journal of experimental & clinical cancer research : CR, 37, 36. doi:10.1186/s13046-018-0704-8. https://pubmed.ncbi.nlm.nih.gov/29471852/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen