C57BL/6JCya-Rbm28em1flox/Cya
Common Name:
Rbm28-flox
Product ID:
S-CKO-17539
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rbm28-flox
Strain ID
CKOCMP-68272-Rbm28-B6J-VC
Gene Name
Product ID
S-CKO-17539
Gene Alias
2810480G15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbm28em1flox/Cya mice (Catalog S-CKO-17539) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007993
NCBI RefSeq
NM_133925
Target Region
Exon 4~6
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Rbm28, also known as RNA-binding motif protein 28, is a nucleolar component of spliceosomal small nuclear ribonucleoproteins. It is involved in the nucleolar stress response, and its yeast ortholog Nop4p is known to regulate ribosome biogenesis [4,8]. Rbm28 is also associated with pathways related to tumor suppression, hair follicle growth, and angiogenesis [1,3,6].
In cancer, Rbm28 is frequently overexpressed, promoting cancer cell survival and growth by inhibiting the transcriptional activity of the tumor suppressor p53. For example, upon treatment with chemotherapeutic drugs, Rbm28 translocates from the nucleolus to the nucleoplasm, likely mediated by phosphorylation at Ser122 by Chk1/2 [1]. In colorectal cancer, USP36 stabilizes Rbm28 via deubiquitination, which in turn suppresses p53 transcriptional activity [2]. In hepatocellular carcinoma, increased Rbm28 expression enhances angiogenesis by improving STAT3 translation [6]. In ANE syndrome, a rare inherited disorder, decreased expression of Rbm28 leads to alopecia, neurological defects, and endocrinopathy. In human hair follicle organ cultures, down-regulation of Rbm28 results in catagen induction and hair growth arrest, and Rbm28 is shown to regulate miR-203 and p63 which are important for hair morphogenesis [3,5,7,8].
In conclusion, Rbm28 plays crucial roles in multiple biological processes. Its overexpression in cancers promotes tumor progression, while its deficiency causes ANE syndrome. Studies on Rbm28, including those using potential genetic models like KO or CKO mouse models (although not directly mentioned in the given references), could further clarify its functions and potentially lead to new therapeutic strategies for related diseases.
References:
1. Lin, Xin, Zhou, Liwen, Zhong, Jianliang, Kang, Tiebang, Wu, Yuanzhong. 2021. RNA-binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53. In The Journal of biological chemistry, 298, 101524. doi:10.1016/j.jbc.2021.101524. https://pubmed.ncbi.nlm.nih.gov/34953860/
2. Xu, Hengjie, Wang, Tuo, Nie, Hongxu, Feng, Yifei, Sun, Yueming. 2024. USP36 promotes colorectal cancer progression through inhibition of p53 signaling pathway via stabilizing RBM28. In Oncogene, 43, 3442-3455. doi:10.1038/s41388-024-03178-y. https://pubmed.ncbi.nlm.nih.gov/39343961/
3. Warshauer, Emily, Samuelov, Liat, Sarig, Ofer, Sprecher, Eli, Nousbeck, Janna. 2015. RBM28, a protein deficient in ANE syndrome, regulates hair follicle growth via miR-203 and p63. In Experimental dermatology, 24, 618-22. doi:10.1111/exd.12737. https://pubmed.ncbi.nlm.nih.gov/25939713/
4. Damianov, Andrey, Kann, Michael, Lane, William S, Bindereif, Albrecht. . Human RBM28 protein is a specific nucleolar component of the spliceosomal snRNPs. In Biological chemistry, 387, 1455-60. doi:. https://pubmed.ncbi.nlm.nih.gov/17081119/
5. Bryant, Carson J, Lorea, Cláudia F, de Almeida, Hiram Larangeira, Pinto E Vairo, Filippo, Baserga, Susan J. . Biallelic splicing variants in the nucleolar 60S assembly factor RBM28 cause the ribosomopathy ANE syndrome. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2017777118. https://pubmed.ncbi.nlm.nih.gov/33941690/
6. Han, Hexu, Yuan, Yin, Li, Caiying, Lin, Mei, Huang, Junxing. 2024. RNA-binding motif protein 28 enhances angiogenesis by improving STAT3 translation in hepatocellular carcinoma. In Cancer letters, 604, 217191. doi:10.1016/j.canlet.2024.217191. https://pubmed.ncbi.nlm.nih.gov/39181434/
7. Spiegel, Ronen, Shalev, Stavit A, Adawi, Amin, Sprecher, Eli, Tenenbaum-Rakover, Yardena. 2010. ANE syndrome caused by mutated RBM28 gene: a novel etiology of combined pituitary hormone deficiency. In European journal of endocrinology, 162, 1021-5. doi:10.1530/EJE-10-0077. https://pubmed.ncbi.nlm.nih.gov/20231366/
8. Nousbeck, Janna, Spiegel, Ronen, Ishida-Yamamoto, Akemi, Shalev, Stavit, Sprecher, Eli. 2008. Alopecia, neurological defects, and endocrinopathy syndrome caused by decreased expression of RBM28, a nucleolar protein associated with ribosome biogenesis. In American journal of human genetics, 82, 1114-21. doi:10.1016/j.ajhg.2008.03.014. https://pubmed.ncbi.nlm.nih.gov/18439547/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen