C57BL/6JCya-Tmed7em1flox/Cya
Common Name:
Tmed7-flox
Product ID:
S-CKO-17557
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Tmed7-flox
Strain ID
CKOCMP-66676-Tmed7-B6J-VB
Gene Name
Product ID
S-CKO-17557
Gene Alias
3930401E15Rik; 5830493P14Rik; TRAM
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmed7em1flox/Cya mice (Catalog S-CKO-17557) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000151189
NCBI RefSeq
NM_025698
Target Region
Exon 2~3
Size of Effective Region
~5.4 kb
Detailed Document
Overview of Gene Research
Tmed7, short for transmembrane emp24 domain-containing protein 7, is a COPII adaptor protein. It functions as a cargo sorting adaptor in the early secretory pathway, facilitating the anterograde transport of proteins from the endoplasmic reticulum to the Golgi. It is involved in the regulation of innate immunity, especially in Toll-like receptor 4 (TLR4)-mediated inflammatory signaling pathways, and is also associated with sperm maturation and embryo development [2,3,6]. Genetic models are valuable for studying Tmed7 as they can help clarify its precise functions in these processes.
In innate immunity, TMED7 is required for the trafficking of TLR4 from the endoplasmic reticulum to the cell surface. Depletion of TMED7 reduces TLR4 signaling mediated by the adaptor protein MyD88 [4]. RHBDL4, an intramembrane protease, triggers the degradation of TMED7, counteracting TLR4 transport to the cell surface. TLR4 activation also mediates transcriptional upregulation of RHBDL4, forming a negative feedback loop. In a mouse model, this mechanism alleviates septic shock, and a hypomorphic RHBDL4 mutation linked to Kawasaki syndrome further supports the pathophysiological relevance [1]. Overexpression of miR-340-5p, which targets TMED7, can alleviate the injury of A549 cells caused by Mycobacterium tuberculosis infection by inhibiting the activation of NF-κB [5].
In conclusion, Tmed7 plays a crucial role in the regulation of TLR4-mediated inflammatory signaling. Mouse models, especially those related to septic shock and Kawasaki syndrome, have significantly contributed to understanding the role of Tmed7 in disease-related innate immune responses. Its function in protein trafficking also has implications for sperm maturation and embryo development, highlighting its importance in multiple biological processes.
References:
1. Knopf, Julia D, Steigleder, Susanne S, Korn, Friederike, Adrain, Colin, Lemberg, Marius K. 2024. RHBDL4-triggered downregulation of COPII adaptor protein TMED7 suppresses TLR4-mediated inflammatory signaling. In Nature communications, 15, 1528. doi:10.1038/s41467-024-45615-2. https://pubmed.ncbi.nlm.nih.gov/38453906/
2. Hermo, L, Oliveira, R L, Smith, C E, Au, C E, Bergeron, J J M. 2019. Dark side of the epididymis: tails of sperm maturation. In Andrology, 7, 566-580. doi:10.1111/andr.12641. https://pubmed.ncbi.nlm.nih.gov/31102346/
3. Holm, Julia E J, Soares, Sandro G, Symmons, Martyn F, Moncrieffe, Martin C, Gay, Nicholas J. 2023. Anterograde trafficking of Toll-like receptors requires the cargo sorting adaptors TMED-2 and 7. In Traffic (Copenhagen, Denmark), 24, 508-521. doi:10.1111/tra.12912. https://pubmed.ncbi.nlm.nih.gov/37491993/
4. Liaunardy-Jopeace, Ardiyanto, Bryant, Clare E, Gay, Nicholas J. 2014. The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. In Science signaling, 7, ra70. doi:10.1126/scisignal.2005275. https://pubmed.ncbi.nlm.nih.gov/25074978/
5. Zhang, Bailing, Li, Honglang, Zhang, Jieling, Hang, Yaping, Xu, Yi. 2021. Overexpression of microRNA-340-5p Ameliorates Inflammatory Response and Intracellular Survival of Mycobacterium Tuberculosis in Alveolar Type II Cells. In Infection and drug resistance, 14, 1573-1584. doi:10.2147/IDR.S291867. https://pubmed.ncbi.nlm.nih.gov/33911883/
6. Nie, Zheng-Wen, Niu, Ying-Jie, Zhou, Wenjun, Kim, Ju-Yeon, Cui, Xiang-Shun. 2020. AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos. In Journal of cell science, 133, . doi:10.1242/jcs.243238. https://pubmed.ncbi.nlm.nih.gov/33148610/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen