Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Tmed7em1flox/Cya
Common Name:
Tmed7-flox
Product ID:
S-CKO-17557
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tmed7-flox
Strain ID
CKOCMP-66676-Tmed7-B6J-VB
Gene Name
Tmed7
Product ID
S-CKO-17557
Gene Alias
3930401E15Rik; 5830493P14Rik; TRAM
Background
C57BL/6JCya
NCBI ID
66676
Modification
Conditional knockout
Chromosome
18
Phenotype
MGI:1913926
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmed7em1flox/Cya mice (Catalog S-CKO-17557) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000151189
NCBI RefSeq
NM_025698
Target Region
Exon 2~3
Size of Effective Region
~5.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Tmed7, short for transmembrane emp24 domain-containing protein 7, is a COPII adaptor protein. It functions as a cargo sorting adaptor in the early secretory pathway, facilitating the anterograde transport of proteins from the endoplasmic reticulum to the Golgi. It is involved in the regulation of innate immunity, especially in Toll-like receptor 4 (TLR4)-mediated inflammatory signaling pathways, and is also associated with sperm maturation and embryo development [2,3,6]. Genetic models are valuable for studying Tmed7 as they can help clarify its precise functions in these processes.

In innate immunity, TMED7 is required for the trafficking of TLR4 from the endoplasmic reticulum to the cell surface. Depletion of TMED7 reduces TLR4 signaling mediated by the adaptor protein MyD88 [4]. RHBDL4, an intramembrane protease, triggers the degradation of TMED7, counteracting TLR4 transport to the cell surface. TLR4 activation also mediates transcriptional upregulation of RHBDL4, forming a negative feedback loop. In a mouse model, this mechanism alleviates septic shock, and a hypomorphic RHBDL4 mutation linked to Kawasaki syndrome further supports the pathophysiological relevance [1]. Overexpression of miR-340-5p, which targets TMED7, can alleviate the injury of A549 cells caused by Mycobacterium tuberculosis infection by inhibiting the activation of NF-κB [5].

In conclusion, Tmed7 plays a crucial role in the regulation of TLR4-mediated inflammatory signaling. Mouse models, especially those related to septic shock and Kawasaki syndrome, have significantly contributed to understanding the role of Tmed7 in disease-related innate immune responses. Its function in protein trafficking also has implications for sperm maturation and embryo development, highlighting its importance in multiple biological processes.

References:
1. Knopf, Julia D, Steigleder, Susanne S, Korn, Friederike, Adrain, Colin, Lemberg, Marius K. 2024. RHBDL4-triggered downregulation of COPII adaptor protein TMED7 suppresses TLR4-mediated inflammatory signaling. In Nature communications, 15, 1528. doi:10.1038/s41467-024-45615-2. https://pubmed.ncbi.nlm.nih.gov/38453906/
2. Hermo, L, Oliveira, R L, Smith, C E, Au, C E, Bergeron, J J M. 2019. Dark side of the epididymis: tails of sperm maturation. In Andrology, 7, 566-580. doi:10.1111/andr.12641. https://pubmed.ncbi.nlm.nih.gov/31102346/
3. Holm, Julia E J, Soares, Sandro G, Symmons, Martyn F, Moncrieffe, Martin C, Gay, Nicholas J. 2023. Anterograde trafficking of Toll-like receptors requires the cargo sorting adaptors TMED-2 and 7. In Traffic (Copenhagen, Denmark), 24, 508-521. doi:10.1111/tra.12912. https://pubmed.ncbi.nlm.nih.gov/37491993/
4. Liaunardy-Jopeace, Ardiyanto, Bryant, Clare E, Gay, Nicholas J. 2014. The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane. In Science signaling, 7, ra70. doi:10.1126/scisignal.2005275. https://pubmed.ncbi.nlm.nih.gov/25074978/
5. Zhang, Bailing, Li, Honglang, Zhang, Jieling, Hang, Yaping, Xu, Yi. 2021. Overexpression of microRNA-340-5p Ameliorates Inflammatory Response and Intracellular Survival of Mycobacterium Tuberculosis in Alveolar Type II Cells. In Infection and drug resistance, 14, 1573-1584. doi:10.2147/IDR.S291867. https://pubmed.ncbi.nlm.nih.gov/33911883/
6. Nie, Zheng-Wen, Niu, Ying-Jie, Zhou, Wenjun, Kim, Ju-Yeon, Cui, Xiang-Shun. 2020. AGS3-dependent trans-Golgi network membrane trafficking is essential for compaction in mouse embryos. In Journal of cell science, 133, . doi:10.1242/jcs.243238. https://pubmed.ncbi.nlm.nih.gov/33148610/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest