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C57BL/6JCya-Itga4em1flox/Cya
Common Name:
Itga4-flox
Product ID:
S-CKO-17560
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Itga4-flox
Strain ID
CKOCMP-16401-Itga4-B6J-VC
Gene Name
Itga4
Product ID
S-CKO-17560
Gene Alias
CD49D; Itga4B
Background
C57BL/6JCya
NCBI ID
16401
Modification
Conditional knockout
Chromosome
2
Phenotype
MGI:96603
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itga4em1flox/Cya mice (Catalog S-CKO-17560) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099972
NCBI RefSeq
NM_010576
Target Region
Exon 3~5
Size of Effective Region
~6.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Itga4, also known as Integrin Subunit Alpha 4, is a member of the integrin protein family. Integrins are cell adhesion molecules that play crucial roles in cell-cell and cell-extracellular matrix interactions. Itga4 is involved in multiple biological processes such as cell adhesion, migration, and immune regulation, and is associated with pathways like PI3K-Akt and TSC-mTOR [5].

In pancreatic cancer, the LAMA5/ITGA4 axis in cancer-associated fibroblasts (CAFs) is responsible for CAF-mediated acinar-to-ductal cell transdifferentiation, highlighting its role in pancreatic carcinogenesis [1].

In chronic lymphocytic leukemia (CLL), hypermethylation at ITGA4 gene CpG sites (1, 2, 3) is a characteristic feature [2].

In aging muscle, the chemokine/ITGA4 interaction directs iPSC-derived myogenic progenitor migration to injury sites for regeneration [3].

In inflammatory bowel disease, associated variants of ITGA4 are correlated with expression changes in response to immune stimulus [4].

In gastric cancer, ITGA4 promotes tumor proliferation and migration, and is related to poor prognosis [5,7].

In colorectal cancer, low ITGA4 expression is related to poor prognosis, and ITGA4 might participate in the inflammatory reaction and inflammatory tumor transformation process [6].

In glioma, GAS41 promotes ITGA4-mediated PI3K/Akt/mTOR signaling pathway and glioma tumorigenesis [8].

In conclusion, Itga4 is essential for multiple biological processes including cell adhesion, migration, and immune regulation. Studies, especially those using gene-based models in various diseases like pancreatic cancer, leukemia, muscle regeneration-related conditions, inflammatory bowel disease, gastric cancer, colorectal cancer, and glioma, have revealed its significant roles in disease development and progression. These findings provide potential insights for disease treatment and prevention targeting Itga4.

References:
1. Parte, Seema, Kaur, Annant B, Nimmakayala, Rama Krishna, Batra, Surinder K, Ponnusamy, Moorthy P. 2023. Cancer-Associated Fibroblast Induces Acinar-to-Ductal Cell Transdifferentiation and Pancreatic Cancer Initiation Via LAMA5/ITGA4 Axis. In Gastroenterology, 166, 842-858.e5. doi:10.1053/j.gastro.2023.12.018. https://pubmed.ncbi.nlm.nih.gov/38154529/
2. Attia, Hanaa Rm, Ibrahim, Mona Hamed, El-Aziz, Shereen H Abd, Salama, Iman I, Sobeih, Mohamed Emam. 2020. ITGA4 gene methylation status in chronic lymphocytic leukemia. In Future science OA, 6, FSO583. doi:10.2144/fsoa-2020-0034. https://pubmed.ncbi.nlm.nih.gov/32802392/
3. Ashraf, Muhammad, Tipparaju, Srinivas M, Kim, Joung Woul, Xuan, Wanling. 2023. Chemokine/ITGA4 Interaction Directs iPSC-Derived Myogenic Progenitor Migration to Injury Sites in Aging Muscle for Regeneration. In Cells, 12, . doi:10.3390/cells12141837. https://pubmed.ncbi.nlm.nih.gov/37508502/
4. de Lange, Katrina M, Moutsianas, Loukas, Lee, James C, Anderson, Carl A, Barrett, Jeffrey C. 2017. Genome-wide association study implicates immune activation of multiple integrin genes in inflammatory bowel disease. In Nature genetics, 49, 256-261. doi:10.1038/ng.3760. https://pubmed.ncbi.nlm.nih.gov/28067908/
5. Zhang, Jiaxing, Wang, Gang, Liu, Jie, Wang, Song, Li, Yumin. 2025. ITGA4 as a potential prognostic and immunotherapeutic biomarker in human cancer and its clinical significance in gastric cancer: an integrated analysis and validation. In Frontiers in oncology, 15, 1513622. doi:10.3389/fonc.2025.1513622. https://pubmed.ncbi.nlm.nih.gov/40012546/
6. Mo, Jiahao, Zhang, Jiahui, Huang, Haocheng, Zhong, Cailing, Zhang, Beiping. . The early predictive effect of low expression of the ITGA4 in colorectal cancer. In Journal of gastrointestinal oncology, 13, 265-278. doi:10.21037/jgo-22-92. https://pubmed.ncbi.nlm.nih.gov/35284127/
7. Fang, Tianyi, Yin, Xin, Wang, Yufei, Wang, Xibo, Xue, Yingwei. 2022. Lymph Node Metastasis-Related Gene ITGA4 Promotes the Proliferation, Migration, and Invasion of Gastric Cancer Cells by Regulating Tumor Immune Microenvironment. In Journal of oncology, 2022, 1315677. doi:10.1155/2022/1315677. https://pubmed.ncbi.nlm.nih.gov/36254221/
8. Shang, Guanglei, Zhang, Wenju, Jia, Yanjie, Li, Yunqian, Zeng, Lei. 2025. GAS41 promotes ITGA4-mediated PI3K/Akt/mTOR signaling pathway and glioma tumorigenesis. In Biochemical pharmacology, 233, 116747. doi:10.1016/j.bcp.2025.116747. https://pubmed.ncbi.nlm.nih.gov/39788387/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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