C57BL/6NCya-Bap1em1flox/Cya
Common Name:
Bap1-flox
Product ID:
S-CKO-17593
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Bap1-flox
Strain ID
CKOCMP-104416-Bap1-B6N-VB
Gene Name
Product ID
S-CKO-17593
Gene Alias
2300006C11Rik; mKIAA0272; uch-x4
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Bap1em1flox/Cya mice (Catalog S-CKO-17593) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022458
NCBI RefSeq
NM_027088
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
BAP1, short for BRCA1-Associated Protein 1, is a ubiquitin carboxy-terminal hydrolase functioning as a tumor suppressor [1,3,4,6]. It regulates multiple crucial processes via its deubiquitinating activity, such as DNA damage repair, cell cycle control, chromatin modification, programmed cell death, and the immune response [1]. BAP1-related pathways are involved in various biological processes and are of great significance in maintaining normal cellular functions.
In functional studies, BAP1 has been shown to decrease histone 2A ubiquitination (H2Aub) on chromatin [2]. It links metabolic regulation of ferroptosis to tumour suppression by repressing the expression of cystine transporter SLC7A11, leading to elevated lipid peroxidation and ferroptosis [2]. Loss-of-function of BAP1 in normal human cholangiocyte organoids engineered by Nuclease technology results in loss of epithelial characteristics and increased motility, indicating its role in controlling epithelial identity through chromatin accessibility regulation [5].
In conclusion, BAP1 is a key tumor suppressor with diverse functions in regulating chromatin-associated processes, cell metabolism, and cell death. Its loss-of-function models, such as those in human organoids, have revealed its importance in tumor-related biological processes, especially in cancers like uveal melanoma, malignant mesothelioma, and renal cell carcinoma, which are often associated with BAP1 mutations [1,2,3,4,6,7].
References:
1. Louie, Bryan H, Kurzrock, Razelle. 2020. BAP1: Not just a BRCA1-associated protein. In Cancer treatment reviews, 90, 102091. doi:10.1016/j.ctrv.2020.102091. https://pubmed.ncbi.nlm.nih.gov/32877777/
2. Zhang, Yilei, Shi, Jiejun, Liu, Xiaoguang, Li, Wei, Gan, Boyi. 2018. BAP1 links metabolic regulation of ferroptosis to tumour suppression. In Nature cell biology, 20, 1181-1192. doi:10.1038/s41556-018-0178-0. https://pubmed.ncbi.nlm.nih.gov/30202049/
3. Carbone, Michele, Harbour, J William, Brugarolas, James, Yang, Haining, Gaudino, Giovanni. 2020. Biological Mechanisms and Clinical Significance of BAP1 Mutations in Human Cancer. In Cancer discovery, 10, 1103-1120. doi:10.1158/2159-8290.CD-19-1220. https://pubmed.ncbi.nlm.nih.gov/32690542/
4. Masclef, Louis, Ahmed, Oumaima, Estavoyer, Benjamin, Nijnik, Anastasia, Affar, El Bachir. 2021. Roles and mechanisms of BAP1 deubiquitinase in tumor suppression. In Cell death and differentiation, 28, 606-625. doi:10.1038/s41418-020-00709-4. https://pubmed.ncbi.nlm.nih.gov/33462414/
5. Artegiani, Benedetta, van Voorthuijsen, Lisa, Lindeboom, Rik G H, Vermeulen, Michiel, Clevers, Hans. 2019. Probing the Tumor Suppressor Function of BAP1 in CRISPR-Engineered Human Liver Organoids. In Cell stem cell, 24, 927-943.e6. doi:10.1016/j.stem.2019.04.017. https://pubmed.ncbi.nlm.nih.gov/31130514/
6. Kwon, Jongbum, Lee, Daye, Lee, Shin-Ai. 2023. BAP1 as a guardian of genome stability: implications in human cancer. In Experimental & molecular medicine, 55, 745-754. doi:10.1038/s12276-023-00979-1. https://pubmed.ncbi.nlm.nih.gov/37009801/
7. Lalloo, Fiona, Kulkarni, Anju, Chau, Cindy, Tischkowitz, Marc, Hanson, Helen. 2023. Clinical practice guidelines for the diagnosis and surveillance of BAP1 tumour predisposition syndrome. In European journal of human genetics : EJHG, 31, 1261-1269. doi:10.1038/s41431-023-01448-z. https://pubmed.ncbi.nlm.nih.gov/37607989/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen