C57BL/6JCya-Ugt8aem1flox/Cya
Common Name:
Ugt8a-flox
Product ID:
S-CKO-17598
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ugt8a-flox
Strain ID
CKOCMP-22239-Ugt8a-B6J-VC
Gene Name
Product ID
S-CKO-17598
Gene Alias
Cgt; Ugt8; mCerGT
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ugt8aem1flox/Cya mice (Catalog S-CKO-17598) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000057944
NCBI RefSeq
NM_011674.5
Target Region
Exon 2
Size of Effective Region
~1322 bp
Detailed Document
Overview of Gene Research
Ugt8a, also known as uridine diphosphate galactosyltransferase 8A, is potentially involved in glycolipid biosynthesis in vivo, especially in male-specific glycolipid production in the kidney [1]. It may be relevant to sex dependency in kidney diseases and is also associated with sphingolipid metabolism and TGF-β signaling pathway [1,4]. It could play a role in CNS lipid metabolism, specifically in the synthesis of myelin-associated lipids, and might be related to processes like myelin regeneration [5,6].
In a study on aging mice, integrated analysis of the kidney transcriptome identified Ugt8a as a potential enzyme for male-specific glycolipid biosynthesis, and inhibiting UGT8 reduced the levels of relevant glycolipids and inflammatory cytokines in the kidney [1]. In a mouse model of Sandhoff disease, the expression of Ugt8a was explored at different ages, but no significant difference in its expression levels related to myelination was found compared to control mice [2]. In multiple sclerosis experimental models, Ugt8a was among the down-regulated mutual genes [3]. In a non-alcoholic fatty liver disease (NAFLD) mouse model, transcriptomic analysis showed that Ugt8a was an important regulator associated with TGF-β signaling pathway and sphingolipid metabolism, and EAHP treatment affected its expression [4].
In summary, Ugt8a is potentially involved in glycolipid biosynthesis, sphingolipid metabolism, and TGF-β signaling. Mouse models in studies related to aging, neurodegenerative diseases, multiple sclerosis, and NAFLD have provided insights into its role in these biological processes and disease conditions, suggesting its importance in understanding these areas at a molecular level.
References:
1. Tsugawa, Hiroshi, Ishihara, Tomoaki, Ogasa, Kota, Minoda, Aki, Arita, Makoto. 2024. A lipidome landscape of aging in mice. In Nature aging, 4, 709-726. doi:10.1038/s43587-024-00610-6. https://pubmed.ncbi.nlm.nih.gov/38609525/
2. Singh, Kshitiz, Quinville, Brianna M, Mitchell, Melissa, Chen, Zhilin, Walia, Jagdeep S. 2022. Gene Expression Profile in the Sandhoff Mouse Brain with Progression of Age. In Genes, 13, . doi:10.3390/genes13112020. https://pubmed.ncbi.nlm.nih.gov/36360256/
3. Rahmat-Zaie, Roya, Amini, Javad, Haddadi, Mohammad, Sanadgol, Nima, Zendedel, Adib. 2023. TNF-α/STAT1/CXCL10 mutual inflammatory axis that contributes to the pathogenesis of experimental models of multiple sclerosis: A promising signaling pathway for targeted therapies. In Cytokine, 168, 156235. doi:10.1016/j.cyto.2023.156235. https://pubmed.ncbi.nlm.nih.gov/37267677/
4. Zhang, Boyu, Han, Cairong, Zhang, Zhongrui, Gong, Puyang, Gu, Jian. 2024. Integrated lipidomic and transcriptomics to explore the effects of ethyl acetate extract of Herpetospermum pedunculosum on nonalcoholic fatty liver disease in mice. In Journal of ethnopharmacology, 337, 118894. doi:10.1016/j.jep.2024.118894. https://pubmed.ncbi.nlm.nih.gov/39369916/
5. Yoon, Hyesook, Triplet, Erin M, Wurtz, Lincoln, Choi, Chan-Il, Scarisbrick, Isobel A. . Regulation of CNS Lipids by Protease Activated Receptor 1. In Journal of neurochemistry, 169, e70047. doi:10.1111/jnc.70047. https://pubmed.ncbi.nlm.nih.gov/40123504/
6. Wasseff, Sameh K, Scherer, Steven S. 2015. Activated immune response in an inherited leukodystrophy disease caused by the loss of oligodendrocyte gap junctions. In Neurobiology of disease, 82, 86-98. doi:10.1016/j.nbd.2015.05.018. https://pubmed.ncbi.nlm.nih.gov/26051537/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen