C57BL/6NCya-Agtem1flox/Cya
Common Name:
Agt-flox
Product ID:
S-CKO-17605
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Agt-flox
Strain ID
CKOCMP-11606-Agt-B6N-VB
Gene Name
Product ID
S-CKO-17605
Gene Alias
AngI; AngII; Aogen; Serpina8
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Agtem1flox/Cya mice (Catalog S-CKO-17605) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063278
NCBI RefSeq
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Target Region
Exon 2
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Agt, short for angiotensinogen, is a key component of the renin-angiotensin-aldosterone system. This system is crucial for regulating blood pressure, fluid and electrolyte balance, and cardiovascular function [1,5]. The genetic variations of Agt are often studied to understand its role in related physiological and pathological processes.
Targeting Agt may offer a new way to inhibit the renin-angiotensin-aldosterone system pathway. Clinical trials with IONIS-AGT-LRx, an antisense inhibitor of Agt, showed it could significantly reduce Agt levels, with a favorable safety profile, and there were trends in blood pressure reduction [1]. In terms of disease associations, some studies found that Agt gene polymorphisms like M235T and T174M may be related to the risk of diseases such as coronary artery disease, myocardial infarction, and Henoch-Schönlein purpura in certain populations [2,3,6,9]. Agt may also serve as a biomarker in gastric and colorectal cancers, with its overexpression related to poorer prognosis in gastric cancer and promoting tumor progression in colorectal cancer [4,7]. Additionally, a specific Agt mutation causing autosomal recessive renal tubular dysgenesis was studied, and hydrocortisone was found to have a potential therapeutic effect through enhancing the stability and interaction of the truncated Agt protein [8].
In conclusion, Agt plays a vital role in the renin-angiotensin-aldosterone system, impacting blood pressure regulation and fluid-electrolyte balance. Studies on Agt gene polymorphisms and its expression changes contribute to understanding the susceptibility and progression of various diseases, including cardiovascular diseases and cancers. The research on Agt provides insights into potential therapeutic targets and biomarkers for these diseases.
References:
1. Morgan, Erin S, Tami, Yvonne, Hu, Kuolung, Bakris, George L, Tsimikas, Sotirios. 2021. Antisense Inhibition of Angiotensinogen With IONIS-AGT-LRx: Results of Phase 1 and Phase 2 Studies. In JACC. Basic to translational science, 6, 485-496. doi:10.1016/j.jacbts.2021.04.004. https://pubmed.ncbi.nlm.nih.gov/34222719/
2. Kou, Junyan, Huang, Jing. 2022. Associations between AGT M235T Polymorphism and Cancer: An Updated Meta-Analysis. In Journal of the renin-angiotensin-aldosterone system : JRAAS, 2022, 7862709. doi:10.1155/2022/7862709. https://pubmed.ncbi.nlm.nih.gov/35317386/
3. Mao, Song, Huang, Songming. 2014. Association of AGT M235T gene polymorphism with HSP/HSPN risk. In Renal failure, 37, 16-21. doi:10.3109/0886022X.2014.977142. https://pubmed.ncbi.nlm.nih.gov/25350836/
4. Wu, Fanqi, Zhang, Longguo, Wang, Li, Zhang, Dekui. 2022. AGT May Serve as a Prognostic Biomarker and Correlated with Immune Infiltration in Gastric Cancer. In International journal of general medicine, 15, 1865-1878. doi:10.2147/IJGM.S351662. https://pubmed.ncbi.nlm.nih.gov/35264871/
5. Vázquez-Moreno, Miguel, Locia-Morales, Daniel, Peralta-Romero, José, Flores-Alfaro, Eugenia, Valladares-Salgado, Adan. 2020. AGT rs4762 is associated with diastolic blood pressure in Mexicans with diabetic nephropathy. In Journal of diabetes and its complications, 35, 107826. doi:10.1016/j.jdiacomp.2020.107826. https://pubmed.ncbi.nlm.nih.gov/33423909/
6. Zhang, Qian, Huang, Qingning, Wang, Xianen, Wang, Yong, Hua, Xiaofang. . The effect of polymorphisms (M235T and T174M) on the angiotensinogen gene (AGT) in coronary artery disease in the Eastern Asian population: A systematic review and meta-analysis. In Medicine, 101, e29911. doi:10.1097/MD.0000000000029911. https://pubmed.ncbi.nlm.nih.gov/36042680/
7. Chen, Wei, Chen, Yihuan, Zhang, Kai, Dong, Ziming, Lu, Jing. 2021. AGT serves as a potential biomarker and drives tumor progression in colorectal carcinoma. In International immunopharmacology, 101, 108225. doi:10.1016/j.intimp.2021.108225. https://pubmed.ncbi.nlm.nih.gov/34655849/
8. Tseng, Min-Hua, Huang, Shih-Ming, Konrad, Martin, Chiang, Ming-Chou, Lin, Shih-Hua. 2021. Effect of Hydrocortisone on Angiotensinogen (AGT) Mutation-Causing Autosomal Recessive Renal Tubular Dysgenesis. In Cells, 10, . doi:10.3390/cells10040782. https://pubmed.ncbi.nlm.nih.gov/33916187/
9. Li, Yan-Yan, Wang, Hui, Wang, Hao, Zhang, Yang-Yang. 2021. Myocardial Infarction and AGT p.Thr174Met Polymorphism: A Meta-Analysis of 7657 Subjects. In Cardiovascular therapeutics, 2021, 6667934. doi:10.1155/2021/6667934. https://pubmed.ncbi.nlm.nih.gov/34025779/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen