C57BL/6NCya-Akt1em1flox/Cya
Common Name:
Akt1-flox
Product ID:
S-CKO-17606
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Akt1-flox
Strain ID
CKOCMP-11651-Akt1-B6N-VB
Gene Name
Product ID
S-CKO-17606
Gene Alias
Akt; LTR-akt; PKB; PKB/Akt; PKBalpha; Rac
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Akt1em1flox/Cya mice (Catalog S-CKO-17606) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001780
NCBI RefSeq
NM_009652
Target Region
Exon 4~7
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Akt1, also known as protein kinase B (PKB), is a serine/threonine kinase that plays a central role in cell survival pathways. It is a key transducer in the phosphoinositide-3-kinase (PI3K)/AKT pathway, which regulates cell survival and is over-activated in many human cancers. Akt1's activity is often studied through genetic models, which can help reveal its functions in various biological processes and disease conditions.
In cancer research, Akt1 has been found to have multiple impacts. It phosphorylates cytoplasmic malic enzyme 2 (ME2fl) at serine 9, preventing its mitochondrial translocation. This leads to a metabolic switch from mitochondrial metabolism to glycolysis, enhancing the glycolytic capacity of tumor cells in vitro and in vivo [1]. In lung cancer, abnormal activation of Akt1/2 is associated with osimertinib resistance, and costunolide, a dual inhibitor of MEK1 and Akt1/2, can overcome this resistance [2]. In triple-negative breast cancer, Akt1 phosphorylates FDX1, promoting cuproptosis resistance by inhibiting FDX1-induced cuproptosis and aerobic respiration while promoting glycolysis [3]. In pancreatic cancer, DUSP2 can suppress Akt1-mediated apoptosis resistance under hypoxic microenvironment by competing with Akt1 to bind to CSNK2A1 and inhibiting Akt1 phosphorylation [4].
In conclusion, Akt1 is crucial for cell survival and is deeply involved in the metabolic adaptation and survival mechanisms of tumor cells in various cancers. The study of Akt1 using genetic models has provided important insights into the molecular mechanisms of cancer development, which may potentially lead to the development of new therapeutic strategies targeting this key kinase.
References:
1. Chen, Taiqi, Xie, Siyi, Cheng, Jie, Jiang, Peng, Du, Wenjing. 2024. AKT1 phosphorylation of cytoplasmic ME2 induces a metabolic switch to glycolysis for tumorigenesis. In Nature communications, 15, 686. doi:10.1038/s41467-024-44772-8. https://pubmed.ncbi.nlm.nih.gov/38263319/
2. Tian, Xueli, Wang, Rui, Gu, Tingxuan, Lee, Mee-Hyun, Dong, Zigang. 2022. Costunolide is a dual inhibitor of MEK1 and AKT1/2 that overcomes osimertinib resistance in lung cancer. In Molecular cancer, 21, 193. doi:10.1186/s12943-022-01662-1. https://pubmed.ncbi.nlm.nih.gov/36203195/
3. Sun, Zicheng, Xu, Huazhen, Lu, Guanming, Guo, Jianping, Li, Jie. 2025. AKT1 Phosphorylates FDX1 to Promote Cuproptosis Resistance in Triple-Negative Breast Cancer. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 12, e2408106. doi:10.1002/advs.202408106. https://pubmed.ncbi.nlm.nih.gov/39976173/
4. Zhang, Yangyang, Kong, Rui, Yang, Wenbo, Hu, Jisheng, Sun, Bei. 2023. DUSP2 recruits CSNK2A1 to suppress AKT1-mediated apoptosis resistance under hypoxic microenvironment in pancreatic cancer. In Cancer letters, 568, 216288. doi:10.1016/j.canlet.2023.216288. https://pubmed.ncbi.nlm.nih.gov/37390887/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen