C57BL/6JCya-Appbp2em1flox/Cya
Common Name:
Appbp2-flox
Product ID:
S-CKO-17610
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Appbp2-flox
Strain ID
CKOCMP-66884-Appbp2-B6J-VC
Gene Name
Product ID
S-CKO-17610
Gene Alias
1300003O07Rik; PAT1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Appbp2em1flox/Cya mice (Catalog S-CKO-17610) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018625
NCBI RefSeq
NM_025825
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Appbp2, amyloid protein-binding protein 2, is involved in the ubiquitin-proteasome system as a substrate receptor of the Cullin 2-RING ligase (CRL2) complex. It determines the specificity of protein degradation by recognizing C-degrons in substrates for ubiquitin-mediated proteolysis [3]. APPBP2 is associated with multiple biological pathways and has significant biological importance in processes like adipose tissue regulation, cell proliferation, and senescence. Genetic models, such as KO/CKO mouse models, are valuable for studying its functions.
In adipose tissues, the CUL2-APPBP2 ubiquitin E3 ligase complex determines the stability of PRDM16 protein. Inhibition of CUL2-APPBP2 extends the half-life of PRDM16, promoting beige adipocyte biogenesis. In contrast, elevated CUL2-APPBP2 in aged adipose tissues degrades PRDM16, repressing adipocyte thermogenesis. Adipocyte-specific deletion of CUL2-APPBP2 counteracts diet-induced obesity, glucose intolerance, insulin resistance, and dyslipidaemia in mice [1]. In human mesenchymal stem cells (hMSCs), CUL2, through APPBP2, targets and promotes the ubiquitin-proteasome-mediated degradation of TSPYL2, a negative regulator of proliferation. This in turn down-regulates the aging marker P21waf1/cip1, counteracting hMSC senescence [2].
In conclusion, Appbp2 is crucial in the ubiquitin-proteasome-mediated protein degradation process. Its function in adipose tissue regulation, as revealed by mouse models, has implications for metabolic diseases such as obesity, glucose intolerance, insulin resistance, and dyslipidaemia. In addition, its role in hMSC senescence provides a molecular basis for interventions against aging-related diseases.
References:
1. Wang, Qiang, Li, Huixia, Tajima, Kazuki, Hirschhorn, Joel N, Kajimura, Shingo. 2022. Post-translational control of beige fat biogenesis by PRDM16 stabilization. In Nature, 609, 151-158. doi:10.1038/s41586-022-05067-4. https://pubmed.ncbi.nlm.nih.gov/35978186/
2. Huang, Daoyuan, Zhao, Qian, Yang, Kuan, Wang, Si, Liu, Guang-Hui. 2023. CRL2APPBP2-mediated TSPYL2 degradation counteracts human mesenchymal stem cell senescence. In Science China. Life sciences, 67, 460-474. doi:10.1007/s11427-023-2451-3. https://pubmed.ncbi.nlm.nih.gov/38170390/
3. Zhao, Shidong, Olmayev-Yaakobov, Diana, Ru, Wenwen, Zhang, Kaiming, Xu, Chao. 2023. Molecular basis for C-degron recognition by CRL2APPBP2 ubiquitin ligase. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2308870120. doi:10.1073/pnas.2308870120. https://pubmed.ncbi.nlm.nih.gov/37844242/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen