C57BL/6JCya-Bmp4em1flox/Cya
Common Name:
Bmp4-flox
Product ID:
S-CKO-17613
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Bmp4-flox
Strain ID
CKOCMP-12159-Bmp4-B6J-VC
Gene Name
Product ID
S-CKO-17613
Gene Alias
Bmp-4; Bmp2b; Bmp2b-1; Bmp2b1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bmp4em1flox/Cya mice (Catalog S-CKO-17613) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000074077
NCBI RefSeq
NM_007554
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Bmp4, short for Bone Morphogenetic Protein 4, is a crucial protein in the BMP signaling pathway. This pathway is involved in multiple biological processes, including early embryonic morphogenesis, especially in the epiblast, as well as the development of extra-embryonic lineages such as trophectoderm [2,3].
In corneal neovascularization (CNV), Bmp4 inhibits the formation of tip cells by reducing the generation of neutrophil extracellular traps (NETs) through the NADPH oxidase-2 (NOX-2) pathway, disrupting the tip-stalk cell balance and thus inhibiting CNV, suggesting it as a potential therapeutic target [1]. In trophoblast development, BMP4-based protocols can convert pluripotent stem cells into trophoblast in vitro, and understanding its role helps model placental development [2,3]. Regarding non-syndromic cleft lip with or without cleft palate (NSCLP), a meta-analysis showed that BMP4 rs17563 polymorphism may be a risk factor in Chinese and Brazilian populations [4]. BMP4 also enhances cell migration and promotes epithelial-mesenchymal transition (EMT) through the Smad1 signaling pathway, which is associated with NSCLP [7]. In breast cancer, BMP4 suppresses metastasis by inhibiting cholesterol biosynthesis, and it may be a predictive biomarker for statin therapy benefit [5]. In endometrial stromal cells, BMP4 affects decidualization by regulating FOXO1 expression, and its down-regulation may contribute to recurrent spontaneous abortion (RSA) [6]. In glioblastoma, BMP4 inhibits invasion by promoting E-cadherin and claudin expression through SMAD signaling [8]. In human preimplantation embryos, BMP4 supplementation triggers apoptosis [9].
In conclusion, Bmp4 is essential in various biological processes such as embryonic development, cell differentiation, and apoptosis. Its role in diseases like CNV, NSCLP, breast cancer, RSA, and glioblastoma has been revealed through different research models. Understanding Bmp4 not only deepens our knowledge of normal biological functions but also provides potential therapeutic directions for these diseases.
References：
1. Nan, Weijin, He, Yuxi, Shen, Sitong, Wang, Shurong, Zhang, Yan. 2023. BMP4 inhibits corneal neovascularization by interfering with tip cells in angiogenesis. In Experimental eye research, 237, 109680. doi:10.1016/j.exer.2023.109680. https://pubmed.ncbi.nlm.nih.gov/37858608/
2. Roberts, R Michael, Ezashi, Toshihiko, Temple, Jasmine, Soncin, Francesca, Parast, Mana M. 2022. The role of BMP4 signaling in trophoblast emergence from pluripotency. In Cellular and molecular life sciences : CMLS, 79, 447. doi:10.1007/s00018-022-04478-w. https://pubmed.ncbi.nlm.nih.gov/35877048/
3. Li, Yingchun, Parast, Mana M. . BMP4 regulation of human trophoblast development. In The International journal of developmental biology, 58, 239-46. doi:10.1387/ijdb.130341mp. https://pubmed.ncbi.nlm.nih.gov/25023690/
4. Bahrami, Reza, Dastgheib, Seyed Alireza, Niktabar, Seyed Mohammadreza, Bahrololoomi, Zahra, Neamatzadeh, Hossein. 2020. Association of BMP4 rs17563 Polymorphism with Nonsyndromic Cleft Lip with or without Cleft Palate Risk: Literature Review and Comprehensive Meta-Analysis. In Fetal and pediatric pathology, 40, 305-319. doi:10.1080/15513815.2019.1707916. https://pubmed.ncbi.nlm.nih.gov/31909686/
5. Chi, Lap Hing, Redfern, Andrew D, Lim Kam Sian, Terry C C, Daly, Roger J, Anderson, Robin L. 2024. BMP4-Induced Suppression of Breast Cancer Metastasis Is Associated with Inhibition of Cholesterol Biosynthesis. In International journal of molecular sciences, 25, . doi:10.3390/ijms25179160. https://pubmed.ncbi.nlm.nih.gov/39273106/
6. Huang, Yanjie, Dai, Fangfang, Chen, Liping, Liu, Hua, Cheng, Yanxiang. . BMP4 in Human Endometrial Stromal Cells Can Affect Decidualization by Regulating FOXO1 Expression. In Endocrinology, 165, . doi:10.1210/endocr/bqae049. https://pubmed.ncbi.nlm.nih.gov/38679470/
7. Hong, Jia-Wei, Yu, Yue, Wang, Lu-Shan, Zhang, Mei-Rong, Xu, Li-Chun. 2022. BMP4 Regulates EMT to be Involved in non-Syndromic Cleft lip With or Without Palate. In The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association, 60, 1462-1473. doi:10.1177/10556656221105762. https://pubmed.ncbi.nlm.nih.gov/35702016/
8. Zhao, Xiangdong, Sun, Qian, Dou, Changwu, Chen, Qianxue, Liu, Baohui. 2019. BMP4 inhibits glioblastoma invasion by promoting E-cadherin and claudin expression. In Frontiers in bioscience (Landmark edition), 24, 1060-1070. doi:. https://pubmed.ncbi.nlm.nih.gov/30844730/
9. De Paepe, C, Aberkane, A, Dewandre, D, Tournaye, H, Van de Velde, H. 2018. BMP4 plays a role in apoptosis during human preimplantation development. In Molecular reproduction and development, 86, 53-62. doi:10.1002/mrd.23081. https://pubmed.ncbi.nlm.nih.gov/30372558/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen