C57BL/6JCya-Celf1em1flox/Cya
Common Name:
Celf1-flox
Product ID:
S-CKO-17620
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Celf1-flox
Strain ID
CKOCMP-13046-Celf1-B6J-VC
Gene Name
Product ID
S-CKO-17620
Gene Alias
1600010O03Rik; Brunol2; CUG-BP; CUG-BP1; CUGBP; Cugbp1; D2Wsu101e; HNAB50; NAB50
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Celf1em1flox/Cya mice (Catalog S-CKO-17620) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000177642
NCBI RefSeq
NM_001244891
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
CELF1, also known as CUGBP Elav-like family member 1, is an RNA-binding protein that binds to GU-rich elements in mRNA. It plays crucial roles in controlling mRNA splicing, translation, and decay, thus being involved in various physiological and pathological processes [7].
CELF1 has been associated with multiple diseases. In heart-related diseases, overexpression of CELF1 in cardiomyocytes leads to reduced capillary density, down-regulation of Vegfa mRNA, and degradation of connexin 43, contributing to vascular rarefaction and dilated cardiomyopathy [4,8]. In cataract development, CELF1 promotes matrix metalloproteinases gene expression at the transcriptional level and regulates alternative splicing of DNA repair genes in lens epithelial cells [1,2]. In Daphnia magna, CELF1 suppresses Doublesex1 expression, potentially avoiding sexual ambiguity [3]. In the context of total parenteral nutrition, the CELF1/p53 axis leads to intestinal villus atrophy by suppressing the proliferation of intestinal epithelial cells [6]. Also, targeting CELF1 RNA-binding activity with small molecules can control hepatic stellate cell activation and liver fibrosis [5].
In conclusion, CELF1 is a key RNA-binding protein involved in multiple biological processes and disease conditions. Studies on CELF1, especially through in vivo models, have enhanced our understanding of its functions in diseases such as heart failure, cataract, and liver fibrosis, providing potential therapeutic targets for these disorders.
References:
1. Xiao, Jun, Tian, Xin, Jin, Siyan, Song, Meijiao, Zou, He. 2022. CELF1 promotes matrix metalloproteinases gene expression at transcriptional level in lens epithelial cells. In BMC ophthalmology, 22, 122. doi:10.1186/s12886-022-02344-8. https://pubmed.ncbi.nlm.nih.gov/35287612/
2. Xiao, Jun, Jin, Siyan, Wang, Xue, Huang, Ju, Zou, He. 2022. CELF1 Selectively Regulates Alternative Splicing of DNA Repair Genes Associated With Cataract in Human Lens Cell Line. In Biochemical genetics, 61, 1319-1333. doi:10.1007/s10528-022-10324-2. https://pubmed.ncbi.nlm.nih.gov/36585568/
3. Tirta, Yusrifar Kharisma, Adachi, Shungo, Perez, Christelle Alexa Garcia, Kato, Yasuhiko, Watanabe, Hajime. 2022. CELF1 represses Doublesex1 expression via its 5' UTR in the crustacean Daphnia magna. In PloS one, 17, e0275526. doi:10.1371/journal.pone.0275526. https://pubmed.ncbi.nlm.nih.gov/36240182/
4. Chang, Kuei-Ting, Wang, Lee-Hsin, Lin, Yu-Mei, Cheng, Ching-Feng, Wang, Guey-Shin. . CELF1 promotes vascular endothelial growth factor degradation resulting in impaired microvasculature in heart failure. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35, e21512. doi:10.1096/fj.202002553R. https://pubmed.ncbi.nlm.nih.gov/33811692/
5. Tan, Yang, Sun, Xueqing, Xu, Yizhu, Xu, Qiang, Wu, Xingxin. . Small molecule targeting CELF1 RNA-binding activity to control HSC activation and liver fibrosis. In Nucleic acids research, 50, 2440-2451. doi:10.1093/nar/gkac139. https://pubmed.ncbi.nlm.nih.gov/35234905/
6. Yan, Jun-Kai, Zhang, Tian, Dai, Li-Na, Cai, Wei, Wang, Ying. 2018. CELF1/p53 axis: a sustained antiproliferative signal leading to villus atrophy under total parenteral nutrition. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33, 3378-3391. doi:10.1096/fj.201801695R. https://pubmed.ncbi.nlm.nih.gov/30514107/
7. Qin, Wan-Jia, Shi, Jin-Jin, Chen, Ru-Yi, Cao, Jia-Feng, Chen, Jiong. 2024. Curriculum vitae of CUG binding protein 1 (CELF1) in homeostasis and diseases: a systematic review. In Cellular & molecular biology letters, 29, 32. doi:10.1186/s11658-024-00556-y. https://pubmed.ncbi.nlm.nih.gov/38443798/
8. Chang, Kuei-Ting, Cheng, Ching-Feng, King, Pei-Chih, Liu, Shin-Yi, Wang, Guey-Shin. 2017. CELF1 Mediates Connexin 43 mRNA Degradation in Dilated Cardiomyopathy. In Circulation research, 121, 1140-1152. doi:10.1161/CIRCRESAHA.117.311281. https://pubmed.ncbi.nlm.nih.gov/28874395/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen