C57BL/6NCya-Cep44em1flox/Cya
Common Name:
Cep44-flox
Product ID:
S-CKO-17621
Background:
C57BL/6NCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Cep44-flox
Strain ID
CKOCMP-382010-Cep44-B6N-VB
Gene Name
Product ID
S-CKO-17621
Gene Alias
4933440G23Rik; mKIAA1712
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cep44em1flox/Cya mice (Catalog S-CKO-17621) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040330
NCBI RefSeq
NM_001009951
Target Region
Exon 3~4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Cep44 is a protein-coding gene. It has crucial functions in centrosome-related processes. Centrosomes are essential for microtubule organization and duplicate once per cell cycle. Cep44 is involved in pathways related to centrosome cohesion and centriole-to-centrosome conversion, which are of great biological importance for cell division and maintaining cell structure [1,2].
In human cells, Cep44 localizes to the proximal end of centrioles. Its ablation leads to loss of centrosome cohesion as it associates with rootletin and regulates its stability and localization to the centrosome [1]. Depletion of Cep44 affects centriole structure and centriole-to-centrosome conversion, despite CEP295 binding to centrioles. This indicates that Cep44 is necessary for the proper formation of the centriole wall, which is a requirement for centriole-to-centrosome conversion [2].
In conclusion, Cep44 plays essential roles in centrosome cohesion and centriole-to-centrosome conversion. These functions are crucial for normal cell division processes. Understanding Cep44's functions through model-based research, especially in relation to centrosome-related mechanisms, may provide insights into potential disease mechanisms associated with abnormal cell division, although no direct disease-specific associations from knockout models were given in the provided references [1,2].
References:
1. Hossain, Delowar, Shih, Sunny Y-P, Xiao, Xintong, White, Julia, Tsang, William Y. 2020. Cep44 functions in centrosome cohesion by stabilizing rootletin. In Journal of cell science, 133, . doi:10.1242/jcs.239616. https://pubmed.ncbi.nlm.nih.gov/31974111/
2. Atorino, Enrico S, Hata, Shoji, Funaya, Charlotta, Neuner, Annett, Schiebel, Elmar. 2020. CEP44 ensures the formation of bona fide centriole wall, a requirement for the centriole-to-centrosome conversion. In Nature communications, 11, 903. doi:10.1038/s41467-020-14767-2. https://pubmed.ncbi.nlm.nih.gov/32060285/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen