C57BL/6NCya-Ell3em1flox/Cya
Common Name:
Ell3-flox
Product ID:
S-CKO-17632
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ell3-flox
Strain ID
CKOCMP-269344-Ell3-B6N-VB
Gene Name
Product ID
S-CKO-17632
Gene Alias
A930015D22Rik
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ell3em1flox/Cya mice (Catalog S-CKO-17632) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000116432
NCBI RefSeq
NM_145973
Target Region
Exon 1~6
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Ell3, an RNA polymerase II elongation factor, is involved in diverse biological processes. It is associated with pathways like the p53-Bcl2 axis, ERK1/2 signaling, and Wnt signaling. Ell3 plays a crucial role in embryonic development, stem cell regulation, and cancer-related processes, highlighting its overall biological importance. Genetic models, especially KO mouse models, are valuable for studying its functions [3-5, 7].
Maternal ELL3 knockout in mice leads to oocyte aneuploidy, subfertility, and embryonic defects. Mechanistically, ELL3 localizes to the spindle during meiosis, and its depletion increases meiotic spindle abnormality. It coordinates with TPX2 to ensure proper function of the microtubule motor KIF11, promoting spindle assembly and chromosome movement [2]. In mouse embryonic stem cells, ELL3 depletion dislodges TET1 and SIN3A from a subset of young LINE-1s (L1Md_Ts), but increases BRD4 enrichment, affecting gene regulation and naïve-primed transition [1].
In conclusion, Ell3 is essential for proper embryonic development, especially in preventing oocyte aneuploidy. Its role in regulating LINE-1-based enhancers in embryonic stem cells also showcases its significance in early development. The gene knockout mouse models have been instrumental in revealing these functions, which are relevant to understanding early miscarriage and embryonic development-related diseases [1,2].
References:
1. Meng, Siyan, Liu, Xiaoxu, Zhu, Shiqi, Luo, Zhuojuan, Lin, Chengqi. 2023. Young LINE-1 transposon 5' UTRs marked by elongation factor ELL3 function as enhancers to regulate naïve pluripotency in embryonic stem cells. In Nature cell biology, 25, 1319-1331. doi:10.1038/s41556-023-01211-y. https://pubmed.ncbi.nlm.nih.gov/37591949/
2. Zhu, Shiqi, Xie, Peng, Yang, Yi, Luo, Zhuojuan, Lin, Chengqi. 2025. Maternal ELL3 loss-of-function leads to oocyte aneuploidy and early miscarriage. In Nature structural & molecular biology, 32, 381-392. doi:10.1038/s41594-024-01471-8. https://pubmed.ncbi.nlm.nih.gov/39820605/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen