C57BL/6NCya-Gprasp1em1flox/Cya
Common Name:
Gprasp1-flox
Product ID:
S-CKO-17642
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gprasp1-flox
Strain ID
CKOCMP-67298-Gprasp1-B6N-VB
Gene Name
Product ID
S-CKO-17642
Gene Alias
2210415K24Rik; 3110031O14Rik; GASP; GASP1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Gprasp1em1flox/Cya mice (Catalog S-CKO-17642) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113144
NCBI RefSeq
NM_026081
Target Region
Exon 11
Size of Effective Region
~4.9 kb
Detailed Document
Overview of Gene Research
Gprasp1, also known as G-protein-coupled receptor-associated sorting protein 1, belongs to the GPRASP/ARMCX protein family. It plays a crucial role in the post-endocytic sorting of G-protein-coupled receptors (GPCRs) by binding to trafficking proteins like Beclin2 and the Dysbindin-HRS-Gαs complex [2]. It is also involved in adaptive responses related to chronic treatments with GPCR agonists [2].
Endothelial Gprasp1 knockout mice have a high probability of cerebral hemorrhage, arteriovenous malformations (AVMs), and exhibit vascular anomalies in multiple organs. Gprasp1 loss-of-function causes endothelial dysfunction in vitro and in vivo, activating GPR4/cAMP/MAPK signalling to disturb endothelial functions [1].
In addition, Gprasp1-and Gprasp2-deficient B-cells accumulate in the germinal center, leading to transcriptional abnormalities, excessive somatic hypermutation, and eventually developing a B-cell hyperproliferative disease. Also, silencing of Gprasp1 increases the survival, quiescence, migration, niche retention, and hematopoietic repopulating activity of hematopoietic stem and progenitor cells post-transplant [3,4].
In conclusion, Gprasp1 is essential for maintaining normal endothelial function, B-cell maturation, and hematopoietic stem cell transplantation. The gene knockout mouse models have revealed its key roles in AVMs and lymphoproliferative diseases, providing insights into the underlying disease mechanisms and potential therapeutic targets.
References:
1. Li, Ruofei, Xiao, Xiao, Yan, Yupeng, Zhang, Hongqi, Wang, Yibo. . GPRASP1 loss-of-function links to arteriovenous malformations by endothelial activating GPR4 signals. In Brain : a journal of neurology, 147, 1571-1586. doi:10.1093/brain/awad335. https://pubmed.ncbi.nlm.nih.gov/37787182/
2. Kaeffer, Juliette, Zeder-Lutz, Gabrielle, Simonin, Frédéric, Lecat, Sandra. . GPRASP/ARMCX Protein Family: Potential Involvement in Health and Diseases Revealed by their Novel Interacting Partners. In Current topics in medicinal chemistry, 21, 227-254. doi:10.2174/1568026620666201202102448. https://pubmed.ncbi.nlm.nih.gov/33267763/
3. Morales-Hernández, Antonio, Kooienga, Emilia, Sheppard, Heather, Chabot, Ashley, McKinney-Freeman, Shannon. 2024. GPRASP protein deficiency triggers lymphoproliferative disease by affecting B-cell differentiation. In HemaSphere, 8, e70037. doi:10.1002/hem3.70037. https://pubmed.ncbi.nlm.nih.gov/39479518/
4. Morales-Hernández, Antonio, Benaksas, Chaïma, Chabot, Ashley, Kang, Guolian, McKinney-Freeman, Shannon. . GPRASP proteins are critical negative regulators of hematopoietic stem cell transplantation. In Blood, 135, 1111-1123. doi:10.1182/blood.2019003435. https://pubmed.ncbi.nlm.nih.gov/32027737/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen