C57BL/6JCya-Hckem1flox/Cya
Common Name:
Hck-flox
Product ID:
S-CKO-17643
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hck-flox
Strain ID
CKOCMP-15162-Hck-B6J-VC
Gene Name
Product ID
S-CKO-17643
Gene Alias
Bmk
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hckem1flox/Cya mice (Catalog S-CKO-17643) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003370
NCBI RefSeq
NM_010407
Target Region
Exon 3~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Hck, short for hematopoietic cell kinase, is a member of the Src-family of non-receptor tyrosine kinases. It is expressed in cells of hematopoietic origin, particularly myelomonocytic cells and B lymphocytes. Hck participates in multiple cell processes such as phagocytosis, adhesion, migration, and actin polymerization. It is also involved in signaling pathways related to cell regulation [2].
In kidney diseases, global or myeloid cell-specific Hck-knockout (KO) attenuates renal inflammation and fibrosis in unilateral ureteral obstruction (UUO) and unilateral ischemia-reperfusion injury (IRI) models. Hck-KO or Hck-inhibitor decreases macrophage M1-like pro-inflammatory polarization, proliferation, and migration, and Hck inhibits autophagy flux in macrophages by interacting with autophagy-related proteins [1].
In pancreatic ductal adenocarcinoma (PDAC), genetic ablation of Hck in myeloid cells impairs PDAC growth and metastasis, reduces the desmoplastic microenvironment, and enhances cytotoxic effector cell infiltration [3].
In acute myeloid leukemia (AML), Hck is highly expressed in leukemia stem cells (LSCs) compared to hematopoietic stem cells (HSCs). Knockdown of Hck leads to cell cycle arrest, decreasing the proliferation and colony formation of human AML cell lines, and Hck is required for leukemogenesis and leukemia maintenance in vivo and in vitro [4].
In conclusion, Hck plays crucial roles in various biological processes and disease conditions. The use of Hck KO mouse models has revealed its significance in renal inflammation and fibrosis, pancreatic cancer, and leukemia. These findings suggest that Hck could be a potential therapeutic target in these disease areas.
References:
1. Chen, Man, Menon, Madhav C, Wang, Wenlin, He, John Cijiang, Wei, Chengguo. 2023. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy. In Nature communications, 14, 4297. doi:10.1038/s41467-023-40086-3. https://pubmed.ncbi.nlm.nih.gov/37463911/
2. Musumeci, F, Schenone, S, Brullo, C, Botta, L, Tintori, C. . Hck inhibitors as potential therapeutic agents in cancer and HIV infection. In Current medicinal chemistry, 22, 1540-64. doi:. https://pubmed.ncbi.nlm.nih.gov/25666803/
3. Poh, Ashleigh R, O'Brien, Megan, Chisanga, David, Nikfarjam, Mehrdad, Ernst, Matthias. . Inhibition of HCK in myeloid cells restricts pancreatic tumor growth and metastasis. In Cell reports, 41, 111479. doi:10.1016/j.celrep.2022.111479. https://pubmed.ncbi.nlm.nih.gov/36223746/
4. Li, Zheng, Wang, Fangce, Tian, Xiaoxue, Xu, Jun, Liang, Aibin. 2021. HCK maintains the self-renewal of leukaemia stem cells via CDK6 in AML. In Journal of experimental & clinical cancer research : CR, 40, 210. doi:10.1186/s13046-021-02007-4. https://pubmed.ncbi.nlm.nih.gov/34167558/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen