C57BL/6NCya-Hpxem1flox/Cya
Common Name:
Hpx-flox
Product ID:
S-CKO-17646
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Hpx-flox
Strain ID
CKOCMP-15458-Hpx-B6N-VB
Gene Name
Product ID
S-CKO-17646
Gene Alias
Hpxn; hx
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Hpxem1flox/Cya mice (Catalog S-CKO-17646) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033185
NCBI RefSeq
NM_017371
Target Region
Exon 5~7
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Hpx, or hemopexin, is a protein-encoding gene. Hemopexin is an important component in the body, mainly involved in heme-binding and transportation, which plays a crucial role in maintaining iron homeostasis and protecting cells from the toxic effects of free heme [4].
In Caenorhabditis elegans, the Hpx-related gene hpx-2 encodes a heme-peroxidase. Loss-of-function experiments showed that hpx-2 mutant animals had a weaker cuticle structure, increased intestinal colonization of E. faecalis, and decreased survival on E. faecalis when a catalytic residue in the peroxidase domain was disrupted. RNA-seq analysis of the hpx-2 mutant revealed changes in genes related to cuticle structure under non-pathogenic conditions and greater differential regulation of infection-response genes under pathogenic conditions, indicating that HPX-2 contributes to pathogen resistance by generating cuticle material in the hypodermis and pharynx [1].
In a rat model of diarrhea-predominant irritable bowel syndrome (IBS-D), treatment with Xingpi Capsule increased the relative expression level of the metabolite HPX. In vitro experiments showed that HPX can alleviate LPS-induced damage in human colonic epithelial cells by inhibiting MAP3K5 and its downstream pathways, suggesting a role in IBS-D treatment [2].
In Korean patients with systemic lupus erythematosus (SLE), urinary HPX levels were significantly elevated, especially in those with lupus nephritis. Follow-up of biomarker levels showed a decrease correlated with changes in SLEDAI-2k scores, indicating its potential as a non-invasive biomarker for tracking disease activity [3].
In conclusion, Hpx and its related proteins play diverse biological functions. In model organisms like C. elegans, it is involved in pathogen resistance, while in mammalian disease models such as IBS-D rats and SLE patients, it is associated with disease-related processes. The study of Hpx using gene-based models helps to understand its role in various biological processes and diseases, potentially providing new insights for disease treatment and biomarker development.
References:
1. Liu, Yi, Kaval, Karan Gautam, van Hoof, Ambro, Garsin, Danielle A. 2019. Heme peroxidase HPX-2 protects Caenorhabditis elegans from pathogens. In PLoS genetics, 15, e1007944. doi:10.1371/journal.pgen.1007944. https://pubmed.ncbi.nlm.nih.gov/30695063/
2. Qian, Weina, Li, Weili, Chen, Xiaoyang, Wang, Yong, Wang, Wei. 2023. Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 111, 154653. doi:10.1016/j.phymed.2023.154653. https://pubmed.ncbi.nlm.nih.gov/36641976/
3. Kim, Ji-Won, Baek, Wook-Young, Jung, Ju-Yang, Lee, Sang-Won, Suh, Chang-Hee. 2024. Longitudinal assessment of urinary ALCAM, HPX, and PRDX6 in Korean patients with systemic lupus erythematosus: implications for disease activity monitoring and treatment response. In Frontiers in immunology, 15, 1369385. doi:10.3389/fimmu.2024.1369385. https://pubmed.ncbi.nlm.nih.gov/38915417/
4. Wei, Xin, Zhang, Fan, Cheng, Dan, Zhang, Wei, Xing, Fei. 2024. Free heme induces neuroinflammation and cognitive impairment by microglial activation via the TLR4/MyD88/NF-κB signaling pathway. In Cell communication and signaling : CCS, 22, 16. doi:10.1186/s12964-023-01387-8. https://pubmed.ncbi.nlm.nih.gov/38183122/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen