C57BL/6NCya-Hpxem1flox/Cya
Common Name
Hpx-flox
Product ID
S-CKO-17646
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-15458-Hpx-B6N-VB
When using this mouse strain in a publication, please cite “Hpx-flox Mouse (Catalog S-CKO-17646) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Hpx-flox
Strain ID
CKOCMP-15458-Hpx-B6N-VB
Gene Name
Product ID
S-CKO-17646
Gene Alias
Hpxn, hx
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000033185
NCBI RefSeq
NM_017371
Target Region
Exon 5~7
Size of Effective Region
~1.8 kb
Overview of Gene Research
Hpx, or hemopexin, is a protein-encoding gene. Hemopexin is an important component in the body, mainly involved in heme-binding and transportation, which plays a crucial role in maintaining iron homeostasis and protecting cells from the toxic effects of free heme [4].
In Caenorhabditis elegans, the Hpx-related gene hpx-2 encodes a heme-peroxidase. Loss-of-function experiments showed that hpx-2 mutant animals had a weaker cuticle structure, increased intestinal colonization of E. faecalis, and decreased survival on E. faecalis when a catalytic residue in the peroxidase domain was disrupted. RNA-seq analysis of the hpx-2 mutant revealed changes in genes related to cuticle structure under non-pathogenic conditions and greater differential regulation of infection-response genes under pathogenic conditions, indicating that HPX-2 contributes to pathogen resistance by generating cuticle material in the hypodermis and pharynx [1].
In a rat model of diarrhea-predominant irritable bowel syndrome (IBS-D), treatment with Xingpi Capsule increased the relative expression level of the metabolite HPX. In vitro experiments showed that HPX can alleviate LPS-induced damage in human colonic epithelial cells by inhibiting MAP3K5 and its downstream pathways, suggesting a role in IBS-D treatment [2].
In Korean patients with systemic lupus erythematosus (SLE), urinary HPX levels were significantly elevated, especially in those with lupus nephritis. Follow-up of biomarker levels showed a decrease correlated with changes in SLEDAI-2k scores, indicating its potential as a non-invasive biomarker for tracking disease activity [3].
In conclusion, Hpx and its related proteins play diverse biological functions. In model organisms like C. elegans, it is involved in pathogen resistance, while in mammalian disease models such as IBS-D rats and SLE patients, it is associated with disease-related processes. The study of Hpx using gene-based models helps to understand its role in various biological processes and diseases, potentially providing new insights for disease treatment and biomarker development.
References：
1. Liu, Yi, Kaval, Karan Gautam, van Hoof, Ambro, Garsin, Danielle A. 2019. Heme peroxidase HPX-2 protects Caenorhabditis elegans from pathogens. In PLoS genetics, 15, e1007944. doi:10.1371/journal.pgen.1007944. https://pubmed.ncbi.nlm.nih.gov/30695063/
2. Qian, Weina, Li, Weili, Chen, Xiaoyang, Wang, Yong, Wang, Wei. 2023. Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 111, 154653. doi:10.1016/j.phymed.2023.154653. https://pubmed.ncbi.nlm.nih.gov/36641976/
3. Kim, Ji-Won, Baek, Wook-Young, Jung, Ju-Yang, Lee, Sang-Won, Suh, Chang-Hee. 2024. Longitudinal assessment of urinary ALCAM, HPX, and PRDX6 in Korean patients with systemic lupus erythematosus: implications for disease activity monitoring and treatment response. In Frontiers in immunology, 15, 1369385. doi:10.3389/fimmu.2024.1369385. https://pubmed.ncbi.nlm.nih.gov/38915417/
4. Wei, Xin, Zhang, Fan, Cheng, Dan, Zhang, Wei, Xing, Fei. 2024. Free heme induces neuroinflammation and cognitive impairment by microglial activation via the TLR4/MyD88/NF-κB signaling pathway. In Cell communication and signaling : CCS, 22, 16. doi:10.1186/s12964-023-01387-8. https://pubmed.ncbi.nlm.nih.gov/38183122/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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