C57BL/6JCya-Mettl22em1flox/Cya
Common Name:
Mettl22-flox
Product ID:
S-CKO-17660
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mettl22-flox
Strain ID
CKOCMP-239706-Mettl22-B6J-VC
Gene Name
Product ID
S-CKO-17660
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mettl22em1flox/Cya mice (Catalog S-CKO-17660) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046470
NCBI RefSeq
NM_146247
Target Region
Exon 4~9
Size of Effective Region
~5.8 kb
Detailed Document
Overview of Gene Research
Mettl22, or Methyltransferase-like 22, is a member of the methyltransferase-like (METTL) family. It functions as a non-histone lysine methyltransferase, methylating the DNA/RNA-binding protein Kin17 on lysine 135, which affects Kin17's association with chromatin [1]. Mettl22 is also involved in hematopoietic stem cell development [2].
In a functional genome-wide RNAi screen, Mettl22 was identified as a gene required for the differentiation of embryonic stem cells into hematopoietic stem/progenitor cells in vitro. In vivo validation in zebrafish confirmed it to be essential for hematopoietic stem/progenitor cell (HSPC) development and for maintenance of human HSCs [2]. Also, in the European sea bass, early exposure to elevated temperature led to an increase in the expression of Mettl22 in early differentiating female gonads, suggesting its role in epigenetic regulatory mechanisms related to sex differentiation [3].
In conclusion, Mettl22 plays crucial roles in hematopoietic stem cell development and potentially in epigenetic regulation during sex differentiation in fish. The discovery of its essentiality in HSPC development through in vitro and in vivo models like zebrafish provides valuable insights into stem cell-related biological processes and may have implications for regenerative medicine.
References：
1. Cloutier, Philippe, Lavallée-Adam, Mathieu, Faubert, Denis, Blanchette, Mathieu, Coulombe, Benoit. 2013. Methylation of the DNA/RNA-binding protein Kin17 by METTL22 affects its association with chromatin. In Journal of proteomics, 100, 115-24. doi:10.1016/j.jprot.2013.10.008. https://pubmed.ncbi.nlm.nih.gov/24140279/
2. Han, Tianxu, Yang, Chao-Shun, Chang, Kung-Yen, Imam, Farhad B, Rana, Tariq M. 2016. Identification of novel genes and networks governing hematopoietic stem cell development. In EMBO reports, 17, 1814-1828. doi:. https://pubmed.ncbi.nlm.nih.gov/27797851/
3. Díaz, Noelia, Piferrer, Francesc. 2015. Lasting effects of early exposure to temperature on the gonadal transcriptome at the time of sex differentiation in the European sea bass, a fish with mixed genetic and environmental sex determination. In BMC genomics, 16, 679. doi:10.1186/s12864-015-1862-0. https://pubmed.ncbi.nlm.nih.gov/26338702/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen