C57BL/6NCya-Mir208aem1flox/Cya
Common Name:
Mir208a-flox
Product ID:
S-CKO-17664
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mir208a-flox
Strain ID
CKOCMP-387204-Mir208a-B6N-VB
Gene Name
Product ID
S-CKO-17664
Gene Alias
Mirn208; Mirn208a; mir-208; mir-208a; mmu-mir-208
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mir208aem1flox/Cya mice (Catalog S-CKO-17664) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083498
NCBI RefSeq
NR_029724
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Mir208a, a cardiac-specific microRNA, plays a key role in modulating gene expression in the heart. It is involved in various cardiac-related processes such as heart development, function, and pathogenesis. It participates in pathways related to cardiac hypertrophy, remodeling, electrophysiology, and the regulation of myofiber genes. Additionally, it may assist in governing systemic energy homeostasis by modulating the "endocrine" function of cardiac muscle [1].
Genetic deletion of miR-208a in rodents attenuated stress-induced cardiac hypertrophy and remodeling, indicating its key role in these processes [1]. Also, mechanical stretch-induced miR-208a expression in cultured rat cardiomyocytes and myoblasts is mediated by TGF-β1, and miR-208a is involved in stretch-induced cardiac hypertrophy and may lead to cardiac fibrosis [2,3]. Blocking the function of miR-208a with anti-miR-208a increased ZEB2 expression in the heart and effectively protected from the development of pathological cardiac hypertrophy, suggesting miR-208a's role in regulating genes related to calcium-handling and contractility [4].
In conclusion, Mir208a is crucial for cardiac function, being deeply involved in processes like cardiac hypertrophy, remodeling, and regulation of calcium-handling genes. Studies using gene knockout (KO) models in rodents have been instrumental in revealing its role in these processes, providing insights into the molecular mechanisms underlying heart diseases.
References:
1. Huang, Xing-Huai, Li, Jia-Lu, Li, Xin-Yue, Liu, Jun, Ding, Jian. 2021. miR-208a in Cardiac Hypertrophy and Remodeling. In Frontiers in cardiovascular medicine, 8, 773314. doi:10.3389/fcvm.2021.773314. https://pubmed.ncbi.nlm.nih.gov/34957257/
2. Wang, Bao-Wei, Wu, Gong-Jhe, Cheng, Wen-Pin, Shyu, Kou-Gi. 2013. Mechanical stretch via transforming growth factor-β1 activates microRNA-208a to regulate hypertrophy in cultured rat cardiac myocytes. In Journal of the Formosan Medical Association = Taiwan yi zhi, 112, 635-43. doi:10.1016/j.jfma.2013.01.002. https://pubmed.ncbi.nlm.nih.gov/24120154/
3. Shyu, Kou-Gi, Wang, Bao-Wei, Wu, Gong-Jhe, Lin, Chiu-Mei, Chang, Hang. 2012. Mechanical stretch via transforming growth factor-β1 activates microRNA208a to regulate endoglin expression in cultured rat cardiac myoblasts. In European journal of heart failure, 15, 36-45. doi:10.1093/eurjhf/hfs143. https://pubmed.ncbi.nlm.nih.gov/22941949/
4. Gladka, Monika M, Kohela, Arwa, de Leeuw, Anne E, van Veen, Toon A B, van Rooij, Eva. . Hypoxia-responsive zinc finger E-box-binding homeobox 2 (ZEB2) regulates a network of calcium-handling genes in the injured heart. In Cardiovascular research, 120, 1869-1883. doi:10.1093/cvr/cvae163. https://pubmed.ncbi.nlm.nih.gov/39308239/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen