C57BL/6JCya-Mir29b-2em1flox/Cya
Common Name:
Mir29b-2-flox
Product ID:
S-CKO-17665
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mir29b-2-flox
Strain ID
CKOCMP-723963-Mir29b-2-B6J-VB
Gene Name
Product ID
S-CKO-17665
Gene Alias
Mirn29b-2; mir-29b-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir29b-2em1flox/Cya mice (Catalog S-CKO-17665) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083478
NCBI RefSeq
NR_029809
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Mir29b-2 is a microRNA. MicroRNAs are small non-coding RNAs that play crucial roles in post-transcriptional regulation of gene expression by binding to target mRNAs, leading to their degradation or translational repression. They are involved in numerous biological processes and disease-related pathways. Genetic models, such as KO/CKO mouse models, can be used to study the function of Mir29b-2.
In Alzheimer's disease research, engineered exosomes containing Mir29b-2 and targeting the somatostatin receptor were developed. These exosomes, produced by gene-modified dendritic cells and expressing CD47 for immune evasion, could effectively reduce presenilin 1 (PSEN1) expression at the translational level. This led to the inhibition of beta-amyloid oligomers production in both cell models and 3xTg-AD animal models, suggesting Mir29b-2's potential in reducing β-amyloid accumulation in Alzheimer's disease [1].
In bovine mastitis studies, the expression levels of Mir29b-2 differed significantly among healthy cows, cows with subclinical mastitis, and cows with clinical mastitis. When combined with the California mastitis test (CMT) and days in milk (DIM) data, Mir29b-2 could be used for screening and classifying milk samples, indicating its potential as a biomarker for bovine mastitis [2].
In conclusion, Mir29b-2 is involved in post-transcriptional gene regulation. Its function has been demonstrated in reducing β-amyloid accumulation in Alzheimer's disease models and as a potential biomarker in bovine mastitis. The use of animal models and engineered exosome systems has helped to uncover these roles, providing insights into potential therapeutic and diagnostic applications for these diseases.
References:
1. Lin, En-Yi, Hsu, Shao-Xi, Wu, Bing-Hua, Harn, Horng-Jyh, Chiou, Tzyy-Wen. 2024. Engineered Exosomes Containing microRNA-29b-2 and Targeting the Somatostatin Receptor Reduce Presenilin 1 Expression and Decrease the β-Amyloid Accumulation in the Brains of Mice with Alzheimer's Disease. In International journal of nanomedicine, 19, 4977-4994. doi:10.2147/IJN.S442876. https://pubmed.ncbi.nlm.nih.gov/38828204/
2. Srikok, Suphakit, Patchanee, Prapas, Boonyayatra, Sukolrat, Chuammitri, Phongsakorn. 2020. Potential role of MicroRNA as a diagnostic tool in the detection of bovine mastitis. In Preventive veterinary medicine, 182, 105101. doi:10.1016/j.prevetmed.2020.105101. https://pubmed.ncbi.nlm.nih.gov/32823253/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen