C57BL/6JCya-Myo6em1flox/Cya
Common Name:
Myo6-flox
Product ID:
S-CKO-17672
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Myo6-flox
Strain ID
CKOCMP-17920-Myo6-B6J-VC
Gene Name
Product ID
S-CKO-17672
Gene Alias
Myo6<rsv>; Tlc; rsv; sv
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myo6em1flox/Cya mice (Catalog S-CKO-17672) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000184480
NCBI RefSeq
NM_001408252
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Myo6, a member of the myosin family, is a unique actin-based motor protein that moves cargo towards the minus ends of actin filaments. It is crucial in multiple biological processes, with its loss or overexpression in humans linked to various pathologies like deafness, cardiomyopathy, neurodegenerative diseases, and cancer. Myo6 interacts with numerous binding partners to mediate distinct functions along the endocytic pathway, and also plays a role in regulating the actin and septin cytoskeleton [1].
In a Myo6WT/C442Y mouse model, AAV-PHP.eB vector-mediated in vivo delivery of Staphylococcus aureus Cas9-single-guide RNA complexes was used to edit the Myo6 gene. The editing rescued auditory function up to 5 months post-injection, as evidenced by improvements in auditory brainstem response, distortion product otoacoustic emission thresholds, cell survival, hair bundle morphology, and calcium levels [2].
In a Myo6 knockout background in a collagen-induced arthritis (CIA) mouse model, MYO6 deficiency attenuated arthritis development and bone destruction, and impaired osteoclast differentiation by inhibiting NFATc1 induction. MYO6 was shown to be essential for podosome organization and endosome transportation in osteoclasts [3].
In conclusion, Myo6 is vital for auditory and vestibular function, and also plays a role in osteoclast-mediated joint damage. Gene editing and knockout mouse models have been instrumental in revealing these functions, providing insights into potential therapeutic strategies for hearing loss and arthritis-related diseases.
References:
1. de Jonge, Janeska J, Batters, Christopher, O'Loughlin, Thomas, Arden, Susan D, Buss, Folma. 2019. The MYO6 interactome: selective motor-cargo complexes for diverse cellular processes. In FEBS letters, 593, 1494-1507. doi:10.1002/1873-3468.13486. https://pubmed.ncbi.nlm.nih.gov/31206648/
2. Xue, Yuanyuan, Hu, Xinde, Wang, Daqi, Zuo, Erwei, Shu, Yilai. 2021. Gene editing in a Myo6 semi-dominant mouse model rescues auditory function. In Molecular therapy : the journal of the American Society of Gene Therapy, 30, 105-118. doi:10.1016/j.ymthe.2021.06.015. https://pubmed.ncbi.nlm.nih.gov/34174443/
3. Tan, Huijing, Ma, Liqing, Qin, Tian, Shen, Xiaoyan, Zhou, Chun. 2023. Myo6 mediates osteoclast function and is essential for joint damage in collagen-induced arthritis. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 166902. doi:10.1016/j.bbadis.2023.166902. https://pubmed.ncbi.nlm.nih.gov/37816396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen