C57BL/6JCya-Pus7lem1flox/Cya
Common Name:
Pus7l-flox
Product ID:
S-CKO-17694
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Pus7l-flox
Strain ID
CKOCMP-78895-Pus7l-B6J-VC
Gene Name
Product ID
S-CKO-17694
Gene Alias
3000003F02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pus7lem1flox/Cya mice (Catalog S-CKO-17694) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049151
NCBI RefSeq
NM_172437
Target Region
Exon 2~6
Size of Effective Region
~12.0 kb
Detailed Document
Overview of Gene Research
Pus7l, a gene related to pseudouridine synthases, is involved in RNA pseudouridine modification. Pseudouridine modification is an important RNA epigenetic modification that can affect RNA stability, function, and various biological processes [3,4].
In a study on sepsis, increased frequency of tryptic phosphopeptides from PUS7L was observed, and increases in mean precursor intensity of peptides from PUS7L were associated with sepsis, suggesting its potential role in the disease [1]. Additionally, rare-variant association analysis revealed that PUS7L has rare-variant associations with age-related hearing loss, indicating its possible involvement in this sensory deficit [2]. Also, in the research on protein-protein interactions, PUS7L was identified as a new interaction partner of AHCY [5]. The CNV of PUS7L was associated with prognosis in hepatocellular carcinoma [3]. In colorectal cancer, the Ψ levels correlated with the expression levels of PUS7L, suggesting its potential significance in the disease [4].
In conclusion, PUS7L is involved in RNA pseudouridine modification and plays roles in multiple diseases including sepsis, age-related hearing loss, hepatocellular carcinoma, and colorectal cancer. Studies on PUS7L contribute to understanding the mechanisms of these diseases and may offer new perspectives for diagnosis and treatment.
References：
1. Thavarajah, Thanusi, Dos Santos, Claudia C, Slutsky, Arthur S, Romaschin, Alexander, Marshall, John G. 2020. The plasma peptides of sepsis. In Clinical proteomics, 17, 26. doi:10.1186/s12014-020-09288-5. https://pubmed.ncbi.nlm.nih.gov/32636717/
2. Cornejo-Sanchez, Diana M, Li, Guangyou, Fabiha, Tabassum, DeWan, Andrew T, Leal, Suzanne M. 2023. Rare-variant association analysis reveals known and new age-related hearing loss genes. In European journal of human genetics : EJHG, 31, 638-647. doi:10.1038/s41431-023-01302-2. https://pubmed.ncbi.nlm.nih.gov/36788145/
3. Jin, Zhipeng, Song, Mengying, Wang, Jianping, Liu, Huayuan, Shi, Guangjun. 2022. Integrative multiomics evaluation reveals the importance of pseudouridine synthases in hepatocellular carcinoma. In Frontiers in genetics, 13, 944681. doi:10.3389/fgene.2022.944681. https://pubmed.ncbi.nlm.nih.gov/36437949/
4. Li, Jiehua, Li, Xuanfei, Zhao, Xiaole, Han, Shaoqing, Zhou, Xiang. 2025. Unveiling the clinical significance of RNA pseudouridine in colorectal cancer. In Science China. Life sciences, , . doi:10.1007/s11427-024-2743-y. https://pubmed.ncbi.nlm.nih.gov/40189490/
5. Lepur, Adriana, Kovačević, Lucija, Belužić, Robert, Vugrek, Oliver. 2016. Combining Unique Multiplex Gateway Cloning and Bimolecular Fluorescence Complementation (BiFC) for High-Throughput Screening of Protein-Protein Interactions. In Journal of biomolecular screening, 21, 1100-1111. doi:10.1177/1087057116659438. https://pubmed.ncbi.nlm.nih.gov/27455993/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen