C57BL/6NCya-Tmem63aem1flox/Cya
Common Name:
Tmem63a-flox
Product ID:
S-CKO-17721
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tmem63a-flox
Strain ID
CKOCMP-208795-Tmem63a-B6N-VC
Gene Name
Product ID
S-CKO-17721
Gene Alias
Tmem64a
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tmem63aem1flox/Cya mice (Catalog S-CKO-17721) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027800
NCBI RefSeq
NM_144794
Target Region
Exon 6
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Tmem63a, or transmembrane protein 63A, functions as a mechanosensitive ion channel. It is involved in multiple biological processes, including lysosomal mechanosensing, surfactant secretion in the lungs, and is linked to pathways related to autophagy in triple-negative breast cancer progression. Its study using genetic models, such as KO mouse models, is crucial for understanding its functions [1,2,3,4].
In Tmem63a knockout mice, genetic deletion led to hypomyelination at postnatal day 14 due to impaired oligodendrocyte precursor cell differentiation, though myelin dysplasia returned to normal by P28, indicating TMEM63A-mediated Ca2+ influx is critical for early myelin development [5]. Loss of Tmem63a and Tmem63b in mice resulted in atelectasis and respiratory failure from surfactant secretion deficit, highlighting its role in lung inflation-induced surfactant secretion [3]. Tmem63a -/- mice also showed reduced mechanical allodynia in inflammatory and post-amputation states, with decreased macrophage infiltration in the dorsal root ganglion, suggesting its role in chronic post-amputation pain regulation [6].
In conclusion, Tmem63a is essential for lysosomal mechanosensing, lung surfactant secretion, oligodendrocyte differentiation, and pain regulation. Studies using KO mouse models have revealed its significance in conditions like hypomyelination, respiratory failure, and chronic post-amputation pain, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Zhang, Tai-Mei, Liao, Li, Yang, Shao-Ying, Shao, Zhi-Min, Li, Da-Qiang. 2022. TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression. In Autophagy, 19, 805-821. doi:10.1080/15548627.2022.2103992. https://pubmed.ncbi.nlm.nih.gov/35920704/
2. Li, Kai, Guo, Yanmeng, Wang, Yayu, Jan, Lily Yeh, Jan, Yuh Nung. 2024. Drosophila TMEM63 and mouse TMEM63A are lysosomal mechanosensory ion channels. In Nature cell biology, 26, 393-403. doi:10.1038/s41556-024-01353-7. https://pubmed.ncbi.nlm.nih.gov/38388853/
3. Chen, Gui-Lan, Li, Jing-Yi, Chen, Xin, Zhang, Jin, Zeng, Bo. 2024. Mechanosensitive channels TMEM63A and TMEM63B mediate lung inflation-induced surfactant secretion. In The Journal of clinical investigation, 134, . doi:10.1172/JCI174508. https://pubmed.ncbi.nlm.nih.gov/38127458/
4. Zheng, Wang, Rawson, Shaun, Shen, Zhangfei, Fu, Tian-Min, Holt, Jeffrey R. 2023. TMEM63 proteins function as monomeric high-threshold mechanosensitive ion channels. In Neuron, 111, 3195-3210.e7. doi:10.1016/j.neuron.2023.07.006. https://pubmed.ncbi.nlm.nih.gov/37543036/
5. Wang, Yue-Ying, Wu, Dan, Zhan, Yongkun, Yu, Yongguo, Shi, Yun Stone. 2025. Cation Channel TMEM63A Autonomously Facilitates Oligodendrocyte Differentiation at an Early Stage. In Neuroscience bulletin, 41, 615-632. doi:10.1007/s12264-024-01338-4. https://pubmed.ncbi.nlm.nih.gov/39982638/
6. Pu, Shaofeng, Wu, Yiyang, Tong, Fang, Han, Qingjian, Du, Dongping. 2022. Mechanosensitive Ion Channel TMEM63A Gangs Up with Local Macrophages to Modulate Chronic Post-amputation Pain. In Neuroscience bulletin, 39, 177-193. doi:10.1007/s12264-022-00910-0. https://pubmed.ncbi.nlm.nih.gov/35821338/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen